The homeobox BcHOX8 gene in Botrytis cinerea regulates vegetative growth and morphology

• Published in: PloS one 2012-10, Vol.7 (10), p.e48134-e48134
• Main Authors: Antal, Zsuzsanna, Rascle, Christine, Cimerman, Agnès, Viaud, Muriel, Billon-Grand, Geneviève, Choquer, Mathias, Bruel, Christophe
• Format: Article
• Language: English
• Subjects: Adaptation; Agricultural sciences; Analysis; Asexual reproduction; Aspergillus nidulans; Biology; Botrytis - genetics; Botrytis cinerea; Conidia; Cushions; DNA Primers - genetics; Enzymes; Expressed Sequence Tags; Fungi; Gene deletion; Gene expression; Gene Expression Regulation, Fungal; Genes; Genes, Fungal; Genes, Homeobox; Genetic aspects; Genome, Fungal; Genomes; Genomics; Growth; Growth rate; Health aspects; Homeobox; Homeodomain Proteins - genetics; Homeodomain Proteins - physiology; Host plants; Hydrophobicity; Infection; Infections; Kinases; Life cycles; Life Sciences; Metabolites; Models, Genetic; Morphology; Mutants; Mutation; Phenotype; Phytopathogenic fungi; Plant Diseases - microbiology; Plants (botany); Sclerotinia sclerotiorum; Transcription (Genetics); Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription Factors - physiology; Virulence
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0048134

Filamentous growth and the capacity at producing conidia are two critical aspects of most fungal life cycles, including that of many plant or animal pathogens. Here, we report on the identification of a homeobox transcription factor encoding gene that plays a role in these two particular aspects of the development of the phytopathogenic fungus Botrytis cinerea. Deletion of the BcHOX8 gene in both the B. cinerea B05-10 and T4 strains causes similar phenotypes, among which a curved, arabesque-like, hyphal growth on hydrophobic surfaces; the mutants were hence named Arabesque. Expression of the BcHOX8 gene is higher in conidia and infection cushions than in developing appressorium or mycelium. In the Arabesque mutants, colony growth rate is reduced and abnormal infection cushions are produced. Asexual reproduction is also affected with abnormal conidiophore being formed, strongly reduced conidia production and dramatic changes in conidial morphology. Finally, the mutation affects the fungus ability to efficiently colonize different host plants. Analysis of the B. cinerea genome shows that BcHOX8 is one member of a nine putative homeobox genes family. Available gene expression data suggest that these genes are functional and sequence comparisons indicate that two of them would be specific to B. cinerea and its close relative Sclerotinia sclerotiorum.