Influence of pericarp, cotyledon and inhibitory substances on sharp tooth oak (Quercus aliena var. acuteserrata) germination

• Published in: PloS one 2012-10, Vol.7 (10), p.e47682-e47682
• Main Authors: Liu, Yan, Liu, Guangquan, Li, Qingmei, Liu, Yong, Hou, Longyu, Li, Guolei
• Format: Article
• Language: English
• Subjects: Agriculture; Biological Assay - methods; Biology; Brassica; Chinese cabbage; Cotyledon; Cotyledons; Dental roots; Distilled water; Ecology; Education; Embryos; Forestry; Germination; Germination - drug effects; Germination - physiology; Laboratories; Metabolites; Methanol; Methanol - chemistry; Morphology; Pericarp; Physiology; Plant Physiological Phenomena; Plant Roots - drug effects; Plant Roots - growth & development; Plant Shoots; Polyethylene; Quercus; Quercus - metabolism; Quercus - physiology; Quercus aliena; Quercus nigra; Quercus robur; Quercus suber; Researchers; Science; Seedlings - drug effects; Seedlings - growth & development; Seeds; Seeds - growth & development; Shoots; Studies; Teeth; Time Factors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0047682

In order to explore the mechanism of delayed and uneven germination in sharp tooth oak (Quercus aliena var. acuteserrata) (STO), mechanical scarification techniques were used to study STO root and shoot germination and growth. The techniques used were: removing cup scar (RS), removing the pericarp (RP), and cutting off 1/2 (HC) and 2/3 (TC) cotyledons. Germination percentage and root and shoot length for Chinese cabbage (Beassica pekinensis) seeds (CCS) were also investigated for CCS cultivated in a Sanyo growth cabinet watered by distilled water and 80% methanol extracts from the acorn embryo, cotyledon and pericarp with concentrations of 1.0 g, 0.8 g, 0.6 g and 0.4 g dry acorn weight per ml methanol. The results showed that the majority of roots and shoots from acorns with RP and HC treatment emerged two weeks earlier, more simultaneously, and their total emergencies were more than 46% and 28% higher, respectively. TC accelerated root and shoot emergence time and root length, but root and shoot germination rate and shoot height had no significant difference from the control. Positive consequences were not observed on all indices of RS treatment. The germination rates of CCS watered by 1.0 g · ml(-1) methanol extracts from the embryo and cotyledon were significantly lower than those from the pericarp, and all concentrations resulted in decreased growth of root and shoot. Methanol extracts from pericarp significantly reduced root length of CCS, but presented little response in germination percentage and shoot length. The inhibitory effect was gradually increased with the increasing concentration of the methanol extract. We conclude that both the mechanical restriction of the pericarp and the presence of germination inhibitors in the embryo, cotyledon and pericarp are the causes for delayed and asynchronous germination of STO acorns.