Self-assembling complexes of quantum dots and scFv antibodies for cancer cell targeting and imaging

Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising ag...

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Bibliographic Details
Published in:PloS one 2012-10, Vol.7 (10), p.e48248
Main Authors: Zdobnova, Tatiana A, Stremovskiy, Oleg A, Lebedenko, Ekaterina N, Deyev, Sergey M
Format: Article
Language:English
Subjects:
Animals
Antibodies
Antibodies - chemistry
Antigens
Bacterial Proteins - chemistry
Barnase
Barstar
Biology
Biomarkers
Biomarkers, Tumor - metabolism
Breast cancer
Cancer
Cell Line, Tumor
Cell Membrane - metabolism
Chemical compounds
Chemical properties
CHO Cells
Cricetinae
Drug resistance
E coli
Electrophoresis
Epidermal growth factor
ErbB-2 protein
Escherichia coli
Escherichia coli - metabolism
Flow Cytometry - methods
Fluorescence
Fluorescent Dyes - pharmacology
Fluorophores
Fluoroscopic imaging
Genetic engineering
Humans
Immunoglobulins
Nanotechnology
Neoplasms - immunology
Neoplasms - metabolism
Proteins
Quantum Dots
Recombinant Proteins - metabolism
Ribonucleases - chemistry
Semiconductors (Materials)
Signal transduction
Single-Chain Antibodies - chemistry
Tissues
Tumor cells
Tumor Cells, Cultured
Tumors
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Summary:Semiconductor quantum dots represent a novel class of fluorophores with unique physical and chemical properties which could enable a remarkable broadening of the current applications of fluorescent imaging and optical diagnostics. Complexes of quantum dots and antibodies are promising visualising agents for fluorescent detection of selective biomarkers overexpressed in tumor tissues. Here we describe the construction of self-assembling fluorescent complexes of quantum dots and anti-HER1 or anti-HER2/neu scFv antibodies and their interactions with cultured tumor cells. A binding strategy based on a very specific non-covalent interaction between two proteins, barnase and barstar, was used to connect quantum dots and the targeting antibodies. Such a strategy allows combining the targeting and visualization functions simply by varying the corresponding modules of the fluorescent complex.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0048248