Selection of Ceratitis capitata (Diptera: Tephritidae) specific recombinant monoclonal phage display antibodies for prey detection analysis

Several recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and...

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Bibliographic Details
Published in:PloS one 2012-12, Vol.7 (12), p.e51440-e51440
Main Authors: Monzó, César, Urbaneja, Alberto, Ximénez-Embún, Miguel, García-Fernández, Julia, García, José Luis, Castañera, Pedro
Format: Article
Language:English
Subjects:
Agriculture
Amino Acid Sequence
Animals
Antibodies, Monoclonal - chemistry
Antigens
Antigens - metabolism
Binding sites
Biological products
Biology
Ceratitis capitata
Ceratitis capitata - immunology
Ceratitis capitata - metabolism
Cloning
Content analysis
Deoxyribonucleic acid
DNA
E coli
Enzyme-Linked Immunosorbent Assay - methods
Female
Immunoglobulins
Laboratory animals
Male
Medicine
Mediterranean fruit fly
Molecular Sequence Data
Monoclonal antibodies
Packaging
Peptide Library
Pests
Phage display
Phages
Plant Diseases
Predation
Predators
Predatory Behavior
Prey
Proteins
Rabbits
Recombinant Proteins - chemistry
Sensitivity and Specificity
Sequence Homology, Amino Acid
Single-Chain Antibodies - chemistry
Studies
Target detection
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Description
Summary:Several recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and sensitivity of the selected recombinant antibodies were compared with that of a rabbit polyclonal serum raised in parallel using a wide range of arthropod species as controls. The selected recombinant monoclonal antibodies had a similar or greater specificity when compared with classical monoclonal antibodies. The selected recombinant antibodies were successfully used to detect the target antigen in the gut of predators and the scFv antibodies were sequenced and compared. These results demonstrate the potential for recombinant scFv antibodies to be used as an alternative to the classical monoclonal antibodies or even molecular probes in the post-mortem analysis studies of generalist predators.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0051440