Evolutionarily conserved histone methylation dynamics during seed life-cycle transitions

• Published in: PloS one 2012-12, Vol.7 (12), p.e51532-e51532
• Main Authors: Müller, Kerstin, Bouyer, Daniel, Schnittger, Arp, Kermode, Allison R
• Format: Article
• Language: English
• Subjects: Angiosperms; Annual variations; Arabidopsis; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - physiology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Biochemistry, Molecular Biology; Biology; Cellular Biology; Chromatin; Chromatin - genetics; Chromatin - physiology; Dormancy; Environmental changes; Environmental conditions; Epigenetics; Evolution, Molecular; Gene expression; Gene Expression Regulation, Plant; Gene silencing; Gene-Environment Interaction; Genomes; Germination; Gymnosperms; Histone-Lysine N-Methyltransferase - genetics; Histone-Lysine N-Methyltransferase - metabolism; Histones - genetics; Histones - metabolism; Life cycle engineering; Life cycles; Life Sciences; Methylation; Molecular biology; Phase transitions; Physcomitrella patens; Plant Dormancy - genetics; Plant Dormancy - physiology; Regulators; Repressor Proteins - genetics; Repressor Proteins - metabolism; Seasonal variations; Seasons; Seedlings; Seedlings - genetics; Seedlings - metabolism; Seedlings - physiology; Seeds; Seeds - growth & development; Seeds - physiology; Synchronism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0051532

Plants have a remarkable ability to react to seasonal changes by synchronizing life-cycle transitions with environmental conditions. We addressed the question of how transcriptional re-programming occurs in response to an environmental cue that triggers the major life cycle transition from seed dormancy to germination and seedling growth. We elucidated an important mechanistic aspect of this process by following the chromatin dynamics of key regulatory genes with a focus on the two antagonistic marks, H3K4me3 and H3K27me3. Histone methylation patterns of major dormancy regulators changed during the transition to germination and seedling growth. We observed a switch from H3K4me3 and high transcription levels to silencing by the repressive H3K27me3 mark when dormancy was broken through exposure to moist chilling, underscoring that a functional PRC2 complex is necessary for this transition. Moreover, this reciprocal regulation by H3K4me3 and H3K27me3 is evolutionarily conserved from gymnosperms to angiosperms.