Polysaccharide from Lentinus edodes inhibits the immunosuppressive function of myeloid-derived suppressor cells

Reversing the function of immune suppressor cells may improve the efficacy of cancer therapy. Here, we have isolated a novel polysaccharide MPSSS (577.2 Kd) from Lentinus edodes and examined its effects on differentiation and function of myeloid-derived suppressor cells (MDSCs). MPSSS is composed of...

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Published in:PloS one 2012-12, Vol.7 (12), p.e51751-e51751
Main Authors: Wu, Hao, Tao, Ning, Liu, Xiaoman, Li, Xiao, Tang, Jian, Ma, Chungwah, Xu, Xiaofei, Shao, Haitao, Hou, Baidong, Wang, Hui, Qin, Zhihai
Format: Article
Language:English
Subjects:
Animals
Antigens, Ly - metabolism
Antineoplastic Agents - pharmacology
Biology
Biophysics
Cancer
Cancer treatment
CD11b Antigen - metabolism
CD4 antigen
Cell Differentiation - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Chromatography
Cytokines
Development and progression
Differentiation
Disease Models, Animal
Flow cytometry
Fungal Polysaccharides - chemistry
Fungal Polysaccharides - isolation & purification
Fungal Polysaccharides - pharmacology
Galactose
Gene expression
Health aspects
Humans
Immunosuppression
Immunosuppressive agents
Immunosuppressive Agents - chemistry
Immunosuppressive Agents - isolation & purification
Immunosuppressive Agents - pharmacology
Laboratories
Lymphocytes
Lymphocytes T
Major histocompatibility complex
Mannose
Medicine
Mice
Molecular weight
Monosaccharides
Morphology
MyD88 protein
Myeloid Cells - cytology
Myeloid Cells - drug effects
Myeloid Cells - immunology
Myeloid Cells - metabolism
Myeloid Differentiation Factor 88 - metabolism
Neoplasms - immunology
Neoplasms - pathology
NF-kappa B - metabolism
NF-κB protein
Peptides
Peripheral blood
Pharmaceuticals
Polysaccharides
Proteins
Rodents
Shedding
Shiitake Mushrooms - chemistry
Signal Transduction
Signaling
Suppressor cells
T cells
T-Lymphocytes - immunology
Tumor Burden - drug effects
Tumor cells
Tumors
Xylose
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cited_by cdi_FETCH-LOGICAL-c692t-2dc1e478e200f5cfc8e39236ef9094219e625a2a3edb9b39299b57f405386e13
cites cdi_FETCH-LOGICAL-c692t-2dc1e478e200f5cfc8e39236ef9094219e625a2a3edb9b39299b57f405386e13
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container_issue 12
container_start_page e51751
container_title PloS one
container_volume 7
creator Wu, Hao
Tao, Ning
Liu, Xiaoman
Li, Xiao
Tang, Jian
Ma, Chungwah
Xu, Xiaofei
Shao, Haitao
Hou, Baidong
Wang, Hui
Qin, Zhihai
description Reversing the function of immune suppressor cells may improve the efficacy of cancer therapy. Here, we have isolated a novel polysaccharide MPSSS (577.2 Kd) from Lentinus edodes and examined its effects on differentiation and function of myeloid-derived suppressor cells (MDSCs). MPSSS is composed of glucose (75.0%), galactose (11.7%), mannose (7.8%), and xylose (0.4%). In vivo, it inhibits the growth of McgR32 tumor cells, which is correlated with a reduced percentage of MDSCs in peripheral blood. In vitro, it induces both morphological and biophysical changes in MDSCs. Importantly, MPSSS up-regulates MHC II and F4/80 expression on MDSCs, and reverses their inhibition effect on CD4(+) T cells in a dose-dependent manner. The mechanism study shows that MPSSS may stimulate MDSCs through a MyD88 dependent NF-κB signaling pathway. Together, we demonstrated for the first time that MPSSS stimulates the differentiation of MDSCs and reverses its immunosuppressive functions, shedding new light on developing novel anti-cancer strategies by targeting MDSCs.
doi_str_mv 10.1371/journal.pone.0051751
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Here, we have isolated a novel polysaccharide MPSSS (577.2 Kd) from Lentinus edodes and examined its effects on differentiation and function of myeloid-derived suppressor cells (MDSCs). MPSSS is composed of glucose (75.0%), galactose (11.7%), mannose (7.8%), and xylose (0.4%). In vivo, it inhibits the growth of McgR32 tumor cells, which is correlated with a reduced percentage of MDSCs in peripheral blood. In vitro, it induces both morphological and biophysical changes in MDSCs. Importantly, MPSSS up-regulates MHC II and F4/80 expression on MDSCs, and reverses their inhibition effect on CD4(+) T cells in a dose-dependent manner. The mechanism study shows that MPSSS may stimulate MDSCs through a MyD88 dependent NF-κB signaling pathway. 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Here, we have isolated a novel polysaccharide MPSSS (577.2 Kd) from Lentinus edodes and examined its effects on differentiation and function of myeloid-derived suppressor cells (MDSCs). MPSSS is composed of glucose (75.0%), galactose (11.7%), mannose (7.8%), and xylose (0.4%). In vivo, it inhibits the growth of McgR32 tumor cells, which is correlated with a reduced percentage of MDSCs in peripheral blood. In vitro, it induces both morphological and biophysical changes in MDSCs. Importantly, MPSSS up-regulates MHC II and F4/80 expression on MDSCs, and reverses their inhibition effect on CD4(+) T cells in a dose-dependent manner. The mechanism study shows that MPSSS may stimulate MDSCs through a MyD88 dependent NF-κB signaling pathway. Together, we demonstrated for the first time that MPSSS stimulates the differentiation of MDSCs and reverses its immunosuppressive functions, shedding new light on developing novel anti-cancer strategies by targeting MDSCs.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23272159</pmid><doi>10.1371/journal.pone.0051751</doi><tpages>e51751</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
ispartof PloS one, 2012-12, Vol.7 (12), p.e51751-e51751
issn 1932-6203
1932-6203
language eng
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source Open Access: PubMed Central; Publicly Available Content Database (Proquest) (PQ_SDU_P3)
subjects Animals
Antigens, Ly - metabolism
Antineoplastic Agents - pharmacology
Biology
Biophysics
Cancer
Cancer treatment
CD11b Antigen - metabolism
CD4 antigen
Cell Differentiation - drug effects
Cell Line, Tumor
Cell Proliferation - drug effects
Chromatography
Cytokines
Development and progression
Differentiation
Disease Models, Animal
Flow cytometry
Fungal Polysaccharides - chemistry
Fungal Polysaccharides - isolation & purification
Fungal Polysaccharides - pharmacology
Galactose
Gene expression
Health aspects
Humans
Immunosuppression
Immunosuppressive agents
Immunosuppressive Agents - chemistry
Immunosuppressive Agents - isolation & purification
Immunosuppressive Agents - pharmacology
Laboratories
Lymphocytes
Lymphocytes T
Major histocompatibility complex
Mannose
Medicine
Mice
Molecular weight
Monosaccharides
Morphology
MyD88 protein
Myeloid Cells - cytology
Myeloid Cells - drug effects
Myeloid Cells - immunology
Myeloid Cells - metabolism
Myeloid Differentiation Factor 88 - metabolism
Neoplasms - immunology
Neoplasms - pathology
NF-kappa B - metabolism
NF-κB protein
Peptides
Peripheral blood
Pharmaceuticals
Polysaccharides
Proteins
Rodents
Shedding
Shiitake Mushrooms - chemistry
Signal Transduction
Signaling
Suppressor cells
T cells
T-Lymphocytes - immunology
Tumor Burden - drug effects
Tumor cells
Tumors
Xylose
title Polysaccharide from Lentinus edodes inhibits the immunosuppressive function of myeloid-derived suppressor cells
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