The Neuronal Calcium Sensor protein Acrocalcin: a potential target of calmodulin regulation during development in the coral Acropora millepora

To understand the calcium-mediated signalling pathways underlying settlement and metamorphosis in the Scleractinian coral Acropora millepora, a predicted protein set derived from larval cDNAs was scanned for the presence of EF-hand domains (Pfam Id: PF00036). This approach led to the identification...

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Bibliographic Details
Published in:PloS one 2012-12, Vol.7 (12), p.e51689-e51689
Main Authors: Reyes-Bermudez, Alejandro, Miller, David J, Sprungala, Susanne
Format: Article
Language:English
Subjects:
Acropora
Acropora millepora
Amino Acid Sequence
Animals
Anthozoa - genetics
Anthozoa - growth & development
Binding sites
Biology
Blotting, Northern
Calcification
Calcium
Calcium - metabolism
Calcium signalling
Calcium-binding protein
Calmodulin
Calmodulin - genetics
Calmodulin - metabolism
Cell cycle
Chromatography, Affinity
Coral reefs
DNA, Complementary - genetics
EF-hand
Gastrulation
Gene Expression Profiling
Gene Expression Regulation, Developmental
Homeostasis
In Situ Hybridization
Larva - cytology
Larva - genetics
Larva - metabolism
Metabolism
Metamorphosis
Metamorphosis, Biological
Molecular Sequence Data
Neuronal Calcium-Sensor Proteins - genetics
Neuronal Calcium-Sensor Proteins - metabolism
Neurons
Pharmacy
Pocillopora damicornis
Protein Binding
Proteins
Real-Time Polymerase Chain Reaction
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA
RNA, Messenger - genetics
Sensors
Sequence Homology, Amino Acid
Signal transduction
Studies
Target recognition
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Summary:To understand the calcium-mediated signalling pathways underlying settlement and metamorphosis in the Scleractinian coral Acropora millepora, a predicted protein set derived from larval cDNAs was scanned for the presence of EF-hand domains (Pfam Id: PF00036). This approach led to the identification of a canonical calmodulin (AmCaM) protein and an uncharacterised member of the Neuronal Calcium Sensor (NCS) family of proteins known here as Acrocalcin (AmAC). While AmCaM transcripts were present throughout development, AmAC transcripts were not detected prior to gastrulation, after which relatively constant mRNA levels were detected until metamorphosis and settlement. The AmAC protein contains an internal CaM-binding site and was shown to interact in vitro with AmCaM. These results are consistent with the idea that AmAC is a target of AmCaM in vivo, suggesting that this interaction may regulate calcium-dependent processes during the development of Acropora millepora.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0051689