Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon)

• Published in: PloS one 2013-01, Vol.8 (1), p.e53926
• Main Authors: Aranda, Ana, Campo, Paloma, Palacin, Arantxa, Doña, Inmaculada, Gomez-Casado, Cristina, Galindo, Luisa, Díaz-Perales, Araceli, Blanca, Miguel
• Format: Article
• Language: English
• Subjects: Activation; Adolescent; Adult; Allergens; Allergens - immunology; Allergens - isolation & purification; Allergies; Analysis; Antigens; Apprenticeship; Asthma; Asthma - blood; Asthma - etiology; Asthma - immunology; Binding; Biology; Biotechnology; Cross-reactivity; Dust; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Exposure; Female; Food allergies; Genomics; Humans; Immunoglobulin E; Immunoglobulin E - blood; Immunoglobulin E - immunology; Immunoglobulin G; Immunoglobulin G - blood; Immunoglobulin G - immunology; In vivo methods and tests; Male; Malvaceae - immunology; Malvaceae - metabolism; Medicine; Middle Aged; Musical groups; Occupational diseases; Occupational Diseases - blood; Occupational Diseases - etiology; Occupational Diseases - immunology; Occupational Exposure - adverse effects; Plant Proteins - immunology; Plant Proteins - isolation & purification; Protein binding; Proteins; Reactivity; Recognition; Rhinitis; Rhinitis - blood; Rhinitis - etiology; Rhinitis - immunology; Skin Tests; Thaumatin; Wood; Wood - immunology; Wood - metabolism; Young Adult
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0053926

Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies. Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p