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Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon)
Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated. To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or...
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| Published in: | PloS one 2013-01, Vol.8 (1), p.e53926 |
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| creator | Aranda, Ana Campo, Paloma Palacin, Arantxa Doña, Inmaculada Gomez-Casado, Cristina Galindo, Luisa Díaz-Perales, Araceli Blanca, Miguel |
| description | Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated.
To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma
An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies.
Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p |
| doi_str_mv | 10.1371/journal.pone.0053926 |
| format | article |
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To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma
An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies.
Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases.
Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0053926</identifier><identifier>PMID: 23349765</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Activation ; Adolescent ; Adult ; Allergens ; Allergens - immunology ; Allergens - isolation & purification ; Allergies ; Analysis ; Antigens ; Apprenticeship ; Asthma ; Asthma - blood ; Asthma - etiology ; Asthma - immunology ; Binding ; Biology ; Biotechnology ; Cross-reactivity ; Dust ; Electrophoresis, Polyacrylamide Gel ; Enzyme-Linked Immunosorbent Assay ; Exposure ; Female ; Food allergies ; Genomics ; Humans ; Immunoglobulin E ; Immunoglobulin E - blood ; Immunoglobulin E - immunology ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; In vivo methods and tests ; Male ; Malvaceae - immunology ; Malvaceae - metabolism ; Medicine ; Middle Aged ; Musical groups ; Occupational diseases ; Occupational Diseases - blood ; Occupational Diseases - etiology ; Occupational Diseases - immunology ; Occupational Exposure - adverse effects ; Plant Proteins - immunology ; Plant Proteins - isolation & purification ; Protein binding ; Proteins ; Reactivity ; Recognition ; Rhinitis ; Rhinitis - blood ; Rhinitis - etiology ; Rhinitis - immunology ; Skin Tests ; Thaumatin ; Wood ; Wood - immunology ; Wood - metabolism ; Young Adult</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e53926</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Aranda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Aranda et al 2013 Aranda et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-18c3cdc2adf339d8da3b78586797340653258698f242a4f0976cd6b2bd827bd43</citedby><cites>FETCH-LOGICAL-c692t-18c3cdc2adf339d8da3b78586797340653258698f242a4f0976cd6b2bd827bd43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1327798999/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1327798999?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23349765$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Uversky, Vladimir N.</contributor><creatorcontrib>Aranda, Ana</creatorcontrib><creatorcontrib>Campo, Paloma</creatorcontrib><creatorcontrib>Palacin, Arantxa</creatorcontrib><creatorcontrib>Doña, Inmaculada</creatorcontrib><creatorcontrib>Gomez-Casado, Cristina</creatorcontrib><creatorcontrib>Galindo, Luisa</creatorcontrib><creatorcontrib>Díaz-Perales, Araceli</creatorcontrib><creatorcontrib>Blanca, Miguel</creatorcontrib><title>Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon)</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Obeche wood dust is a known cause of occupational asthma where an IgE-mediated mechanism has been demonstrated.
To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma
An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies.
Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases.
Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens.</description><subject>Activation</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Allergens</subject><subject>Allergens - immunology</subject><subject>Allergens - isolation & purification</subject><subject>Allergies</subject><subject>Analysis</subject><subject>Antigens</subject><subject>Apprenticeship</subject><subject>Asthma</subject><subject>Asthma - blood</subject><subject>Asthma - etiology</subject><subject>Asthma - immunology</subject><subject>Binding</subject><subject>Biology</subject><subject>Biotechnology</subject><subject>Cross-reactivity</subject><subject>Dust</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Exposure</subject><subject>Female</subject><subject>Food allergies</subject><subject>Genomics</subject><subject>Humans</subject><subject>Immunoglobulin E</subject><subject>Immunoglobulin E - blood</subject><subject>Immunoglobulin E - immunology</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulin G - blood</subject><subject>Immunoglobulin G - immunology</subject><subject>In vivo methods and tests</subject><subject>Male</subject><subject>Malvaceae - immunology</subject><subject>Malvaceae - metabolism</subject><subject>Medicine</subject><subject>Middle Aged</subject><subject>Musical groups</subject><subject>Occupational diseases</subject><subject>Occupational Diseases - blood</subject><subject>Occupational Diseases - etiology</subject><subject>Occupational Diseases - immunology</subject><subject>Occupational Exposure - adverse effects</subject><subject>Plant Proteins - immunology</subject><subject>Plant Proteins - isolation & purification</subject><subject>Protein binding</subject><subject>Proteins</subject><subject>Reactivity</subject><subject>Recognition</subject><subject>Rhinitis</subject><subject>Rhinitis - blood</subject><subject>Rhinitis - etiology</subject><subject>Rhinitis - immunology</subject><subject>Skin Tests</subject><subject>Thaumatin</subject><subject>Wood</subject><subject>Wood - immunology</subject><subject>Wood - metabolism</subject><subject>Young Adult</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNkl2L1DAUhoso7rr6D0QDgrgXM6ZJmzY3wrD4MbCwoKu3IV9tM3SSmqTjzr8343SXKShILvL1nPecnLxZ9jKHyxxX-fuNG73l_XJwVi8hLDFF5FF2nlOMFgRB_PhkfZY9C2FzgGpCnmZnCOOCVqQ8z4aVjabV1kgweBe1sQEYu3P9Tqu0AE7KceDRuJQK-M5YE00A3CrAQ-y2HEg-hoSKPXBCy06DX84p8O7Wm6F3sjPRWRBkr7272_fOXj7PnjS8D_rFNF9k3z99vL36sri--by-Wl0vJKEoLvJaYqkk4qrBmKpacSyquqxJRStcQFJilDa0blCBeNHA9BqpiEBC1agSqsAX2eujbiojsKlZgeUYVRWtKaWJWB8J5fiGDd5sud8zxw37c-B8y7iPJtXORC2hEDkRZS4LDSEVsOYUE0iUFkrApPVhyjaKrVZS2-h5PxOd31jTsdbtGC7LnJZlEngzCXj3c9Qh_qPkiWp5qsrYxiUxuTVBslVR1TmpkjUStfwLlYbSWyOTXRqTzmcBl7OAxER9F9v0tYGtv339f_bmx5x9e8J2mvexC64fD3YKc7A4gtK7ELxuHjqXQ3Zw-3032MHtbHJ7Cnt12vWHoHt7498Brfv6</recordid><startdate>20130122</startdate><enddate>20130122</enddate><creator>Aranda, Ana</creator><creator>Campo, Paloma</creator><creator>Palacin, Arantxa</creator><creator>Doña, Inmaculada</creator><creator>Gomez-Casado, Cristina</creator><creator>Galindo, Luisa</creator><creator>Díaz-Perales, Araceli</creator><creator>Blanca, Miguel</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130122</creationdate><title>Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon)</title><author>Aranda, Ana ; Campo, Paloma ; Palacin, Arantxa ; Doña, Inmaculada ; Gomez-Casado, Cristina ; Galindo, Luisa ; Díaz-Perales, Araceli ; Blanca, Miguel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-18c3cdc2adf339d8da3b78586797340653258698f242a4f0976cd6b2bd827bd43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Activation</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Allergens</topic><topic>Allergens - immunology</topic><topic>Allergens - isolation & purification</topic><topic>Allergies</topic><topic>Analysis</topic><topic>Antigens</topic><topic>Apprenticeship</topic><topic>Asthma</topic><topic>Asthma - blood</topic><topic>Asthma - etiology</topic><topic>Asthma - immunology</topic><topic>Binding</topic><topic>Biology</topic><topic>Biotechnology</topic><topic>Cross-reactivity</topic><topic>Dust</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Exposure</topic><topic>Female</topic><topic>Food allergies</topic><topic>Genomics</topic><topic>Humans</topic><topic>Immunoglobulin E</topic><topic>Immunoglobulin E - blood</topic><topic>Immunoglobulin E - immunology</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulin G - blood</topic><topic>Immunoglobulin G - immunology</topic><topic>In vivo methods and tests</topic><topic>Male</topic><topic>Malvaceae - immunology</topic><topic>Malvaceae - metabolism</topic><topic>Medicine</topic><topic>Middle Aged</topic><topic>Musical groups</topic><topic>Occupational diseases</topic><topic>Occupational Diseases - 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To characterize the allergenic profile of obeche wood dust and evaluate the reactivity of the proteins by in vitro, ex vivo and in vivo assays in carpenters with confirmed rhinitis and/or asthma
An in-house obeche extract was obtained, and two IgE binding bands were purified (24 and 12 kDa) and sequenced by N-terminal identity. Specific IgE and IgG, basophil activation tests and skin prick tests (SPTs) were performed with whole extract and purified proteins. CCD binding was analyzed by ELISA inhibition studies.
Sixty-two subjects participated: 12 with confirmed occupational asthma/rhinitis (ORA+), 40 asymptomatic exposed (ORA-), and 10 controls. Of the confirmed subjects, 83% had a positive SPT to obeche. There was a 100% recognition by ELISA in symptomatic subjects vs. 30% and 10% in asymptomatic exposed subjects and controls respectively (p<0.05). Two new proteins were purified, a 24 kDa protein identified as a putative thaumatin-like protein and a 12 kDa gamma-expansin. Both showed allergenic activity in vitro, with the putative thaumatin being the most active, with 92% recognition by ELISA and 100% by basophil activation test in ORA+ subjects. Cross-reactivity due to CCD was ruled out in 82% of cases.
Two proteins of obeche wood were identified and were recognized by a high percentage of symptomatic subjects and by a small proportion of asymptomatic exposed subjects. Further studies are required to evaluate cross reactivity with other plant allergens.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23349765</pmid><doi>10.1371/journal.pone.0053926</doi><tpages>e53926</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-01, Vol.8 (1), p.e53926 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1327798999 |
| source | Publicly Available Content Database; PubMed Central(OpenAccess) |
| subjects | Activation Adolescent Adult Allergens Allergens - immunology Allergens - isolation & purification Allergies Analysis Antigens Apprenticeship Asthma Asthma - blood Asthma - etiology Asthma - immunology Binding Biology Biotechnology Cross-reactivity Dust Electrophoresis, Polyacrylamide Gel Enzyme-Linked Immunosorbent Assay Exposure Female Food allergies Genomics Humans Immunoglobulin E Immunoglobulin E - blood Immunoglobulin E - immunology Immunoglobulin G Immunoglobulin G - blood Immunoglobulin G - immunology In vivo methods and tests Male Malvaceae - immunology Malvaceae - metabolism Medicine Middle Aged Musical groups Occupational diseases Occupational Diseases - blood Occupational Diseases - etiology Occupational Diseases - immunology Occupational Exposure - adverse effects Plant Proteins - immunology Plant Proteins - isolation & purification Protein binding Proteins Reactivity Recognition Rhinitis Rhinitis - blood Rhinitis - etiology Rhinitis - immunology Skin Tests Thaumatin Wood Wood - immunology Wood - metabolism Young Adult |
| title | Antigenic proteins involved in occupational rhinitis and asthma caused by obeche wood (Triplochiton scleroxylon) |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-27T05%3A17%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Antigenic%20proteins%20involved%20in%20occupational%20rhinitis%20and%20asthma%20caused%20by%20obeche%20wood%20(Triplochiton%20scleroxylon)&rft.jtitle=PloS%20one&rft.au=Aranda,%20Ana&rft.date=2013-01-22&rft.volume=8&rft.issue=1&rft.spage=e53926&rft.pages=e53926-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0053926&rft_dat=%3Cgale_plos_%3EA478167137%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-18c3cdc2adf339d8da3b78586797340653258698f242a4f0976cd6b2bd827bd43%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1327798999&rft_id=info:pmid/23349765&rft_galeid=A478167137&rfr_iscdi=true |