An intact brachyury function is necessary to prevent spurious axial development in Xenopus laevis

We have previously shown that the member of the HES family hairy2 induces the ectopic expression of dorsal markers when it is overexpressed in the ventral side of Xenopus embryos. Intriguingly, hairy2 represses the mesoderm transcription factor brachyury (bra) throughout its domain in the marginal z...

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Bibliographic Details
Published in:PloS one 2013-01, Vol.8 (1), p.e54777-e54777
Main Authors: Aguirre, Cecilia E, Murgan, Sabrina, Carrasco, Andrés E, López, Silvia L
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Axes (reference lines)
Basic Helix-Loop-Helix Transcription Factors - physiology
Biology
Body Patterning
Deoxyribonucleic acid
DNA
Ectopic expression
Embryology
Embryos
Fetal Proteins - physiology
Fetuses
Genes
Health aspects
In Situ Hybridization
Interference
Laboratory animals
Markers
Medicine, Preventive
Mesoderm
Notochord
Preventive health services
Proteins
RNA polymerase
T-Box Domain Proteins - physiology
Xenopus
Xenopus laevis
Xenopus laevis - embryology
Xenopus Proteins - physiology
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Summary:We have previously shown that the member of the HES family hairy2 induces the ectopic expression of dorsal markers when it is overexpressed in the ventral side of Xenopus embryos. Intriguingly, hairy2 represses the mesoderm transcription factor brachyury (bra) throughout its domain in the marginal zone. Here we show that in early gastrula, bra and hairy2 are expressed in complementary domains. Overexpression of bra repressed hairy2. Interference of bra function with a dominant-negative construct expanded the hairy2 domain and, like hairy2 overexpression, promoted ectopic expression of dorsal axial markers in the ventral side and induced secondary axes without head and notochord. Hairy2 depletion rescued the ectopic dorsal development induced by interference of bra function. We concluded that an intact bra function is necessary to exclude hairy2 expression from the non-organiser field, to impede the ectopic specification of dorsal axial fates and the appearance of incomplete secondary axes. This evidence supports a previously unrecognised role for bra in maintaining the dorsal fates inhibited in the ventral marginal zone, preventing the appearance of trunk duplications.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0054777