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Multidisciplinary evidences that Synechocystis PCC6803 exopolysaccharides operate in cell sedimentation and protection against salt and metal stresses
Little is known about the production of exopolysaccharides (EPS) in cyanobacteria, and there are no genetic and physiological evidences that EPS are involved in cell protection against the frequently encountered environmental stresses caused by salt and metals. We studied four presumptive EPS produc...
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Published in: | PloS one 2013-02, Vol.8 (2), p.e55564 |
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description | Little is known about the production of exopolysaccharides (EPS) in cyanobacteria, and there are no genetic and physiological evidences that EPS are involved in cell protection against the frequently encountered environmental stresses caused by salt and metals. We studied four presumptive EPS production genes, sll0923, sll1581, slr1875 and sll5052, in the model cyanobacterium Synechocystis PCC6803, which produces copious amounts of EPS attached to cells (CPS) and released in the culture medium (RPS) as shown here. We show that sll0923, sll1581, slr1875 and sll5052 are all dispensable to the growth of all corresponding single and double deletion mutants in absence of stress. Furthermore, we report that sll0923, sll1581 and slr1875 unambiguously operate in the production of both CPS and RPS. Both sll1581 and slr1875 are more important than sll0923 for CPS production, whereas the contrary is true for RPS production. We show that the most EPS-depleted mutant, doubly deleted for sll1581 and slr1875, lacks the EPS mantle that surrounds WT cells and sorbs iron in their vicinity. Using this mutant, we demonstrate for the first time that cyanobacterial EPS directly operate in cell protection against NaCl, CoCl(2), CdSO(4) and Fe-starvation. We believe that our EPS-depleted mutants will be useful tools to investigate the role of EPS in cell-to-cell aggregation, biofilm formation, biomineralization and tolerance to environmental stresses. We also suggest using the fast sedimenting mutants as biotechnological cell factories to facilitate the otherwise expensive harvest of the producer cell biomass and/or its separation from products excreted in the growth media. |
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We studied four presumptive EPS production genes, sll0923, sll1581, slr1875 and sll5052, in the model cyanobacterium Synechocystis PCC6803, which produces copious amounts of EPS attached to cells (CPS) and released in the culture medium (RPS) as shown here. We show that sll0923, sll1581, slr1875 and sll5052 are all dispensable to the growth of all corresponding single and double deletion mutants in absence of stress. Furthermore, we report that sll0923, sll1581 and slr1875 unambiguously operate in the production of both CPS and RPS. Both sll1581 and slr1875 are more important than sll0923 for CPS production, whereas the contrary is true for RPS production. We show that the most EPS-depleted mutant, doubly deleted for sll1581 and slr1875, lacks the EPS mantle that surrounds WT cells and sorbs iron in their vicinity. Using this mutant, we demonstrate for the first time that cyanobacterial EPS directly operate in cell protection against NaCl, CoCl(2), CdSO(4) and Fe-starvation. We believe that our EPS-depleted mutants will be useful tools to investigate the role of EPS in cell-to-cell aggregation, biofilm formation, biomineralization and tolerance to environmental stresses. We also suggest using the fast sedimenting mutants as biotechnological cell factories to facilitate the otherwise expensive harvest of the producer cell biomass and/or its separation from products excreted in the growth media.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0055564</identifier><identifier>PMID: 23405172</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibiotics ; Bacteria ; Bacteriology ; Biofilms ; Biology ; Biomass ; Cell aggregation ; Cell culture ; Cell growth ; Clonal deletion ; Culture media ; Cyanobacteria ; Deletion mutant ; E coli ; Environmental conditions ; Environmental stress ; Escherichia coli ; Exopolysaccharides ; Genes ; Genetic engineering ; Genomes ; Genomics ; Growth media ; Heavy metals ; Hostages ; Iron ; Life Sciences ; Media (culture) ; Metals ; Metals - toxicity ; Microbiology and Parasitology ; Microscopy, Electron, Scanning ; Mineralization ; Mutagenesis ; Mutants ; Mutation - genetics ; Physiological aspects ; Polysaccharides - pharmacology ; Salts ; Sedimentation ; Sequence Deletion ; Sodium chloride ; Sodium Chloride - toxicity ; Spectroscopy, Fourier Transform Infrared ; Starvation ; Stress, Physiological - drug effects ; Stresses ; Synechocystis ; Synechocystis - drug effects ; Synechocystis - growth & development ; Synechocystis - metabolism</subject><ispartof>PloS one, 2013-02, Vol.8 (2), p.e55564</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Jittawuttipoka et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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We studied four presumptive EPS production genes, sll0923, sll1581, slr1875 and sll5052, in the model cyanobacterium Synechocystis PCC6803, which produces copious amounts of EPS attached to cells (CPS) and released in the culture medium (RPS) as shown here. We show that sll0923, sll1581, slr1875 and sll5052 are all dispensable to the growth of all corresponding single and double deletion mutants in absence of stress. Furthermore, we report that sll0923, sll1581 and slr1875 unambiguously operate in the production of both CPS and RPS. Both sll1581 and slr1875 are more important than sll0923 for CPS production, whereas the contrary is true for RPS production. We show that the most EPS-depleted mutant, doubly deleted for sll1581 and slr1875, lacks the EPS mantle that surrounds WT cells and sorbs iron in their vicinity. Using this mutant, we demonstrate for the first time that cyanobacterial EPS directly operate in cell protection against NaCl, CoCl(2), CdSO(4) and Fe-starvation. We believe that our EPS-depleted mutants will be useful tools to investigate the role of EPS in cell-to-cell aggregation, biofilm formation, biomineralization and tolerance to environmental stresses. We also suggest using the fast sedimenting mutants as biotechnological cell factories to facilitate the otherwise expensive harvest of the producer cell biomass and/or its separation from products excreted in the growth media.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Biofilms</subject><subject>Biology</subject><subject>Biomass</subject><subject>Cell aggregation</subject><subject>Cell culture</subject><subject>Cell growth</subject><subject>Clonal deletion</subject><subject>Culture media</subject><subject>Cyanobacteria</subject><subject>Deletion mutant</subject><subject>E coli</subject><subject>Environmental conditions</subject><subject>Environmental stress</subject><subject>Escherichia coli</subject><subject>Exopolysaccharides</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Growth media</subject><subject>Heavy 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evidences that Synechocystis PCC6803 exopolysaccharides operate in cell sedimentation and protection against salt and metal stresses</title><author>Jittawuttipoka, Thichakorn ; Planchon, Mariane ; Spalla, Olivier ; Benzerara, Karim ; Guyot, François ; Cassier-Chauvat, Corinne ; Chauvat, Franck</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c726t-69aa80cd722a0c53fd8844ee87615ffbe55343108ed0790db3bf03f04db85ae73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacteriology</topic><topic>Biofilms</topic><topic>Biology</topic><topic>Biomass</topic><topic>Cell aggregation</topic><topic>Cell culture</topic><topic>Cell growth</topic><topic>Clonal deletion</topic><topic>Culture media</topic><topic>Cyanobacteria</topic><topic>Deletion mutant</topic><topic>E coli</topic><topic>Environmental conditions</topic><topic>Environmental 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One</addtitle><date>2013-02-06</date><risdate>2013</risdate><volume>8</volume><issue>2</issue><spage>e55564</spage><pages>e55564-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Little is known about the production of exopolysaccharides (EPS) in cyanobacteria, and there are no genetic and physiological evidences that EPS are involved in cell protection against the frequently encountered environmental stresses caused by salt and metals. We studied four presumptive EPS production genes, sll0923, sll1581, slr1875 and sll5052, in the model cyanobacterium Synechocystis PCC6803, which produces copious amounts of EPS attached to cells (CPS) and released in the culture medium (RPS) as shown here. We show that sll0923, sll1581, slr1875 and sll5052 are all dispensable to the growth of all corresponding single and double deletion mutants in absence of stress. Furthermore, we report that sll0923, sll1581 and slr1875 unambiguously operate in the production of both CPS and RPS. Both sll1581 and slr1875 are more important than sll0923 for CPS production, whereas the contrary is true for RPS production. We show that the most EPS-depleted mutant, doubly deleted for sll1581 and slr1875, lacks the EPS mantle that surrounds WT cells and sorbs iron in their vicinity. Using this mutant, we demonstrate for the first time that cyanobacterial EPS directly operate in cell protection against NaCl, CoCl(2), CdSO(4) and Fe-starvation. We believe that our EPS-depleted mutants will be useful tools to investigate the role of EPS in cell-to-cell aggregation, biofilm formation, biomineralization and tolerance to environmental stresses. We also suggest using the fast sedimenting mutants as biotechnological cell factories to facilitate the otherwise expensive harvest of the producer cell biomass and/or its separation from products excreted in the growth media.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23405172</pmid><doi>10.1371/journal.pone.0055564</doi><tpages>e55564</tpages><orcidid>https://orcid.org/0000-0003-4622-2218</orcidid><orcidid>https://orcid.org/0000-0002-0553-0137</orcidid><orcidid>https://orcid.org/0000-0001-6901-5559</orcidid><oa>free_for_read</oa></addata></record> |
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identifier | ISSN: 1932-6203 |
ispartof | PloS one, 2013-02, Vol.8 (2), p.e55564 |
issn | 1932-6203 1932-6203 |
language | eng |
recordid | cdi_plos_journals_1330878137 |
source | PubMed Central Free; Publicly Available Content (ProQuest) |
subjects | Antibiotics Bacteria Bacteriology Biofilms Biology Biomass Cell aggregation Cell culture Cell growth Clonal deletion Culture media Cyanobacteria Deletion mutant E coli Environmental conditions Environmental stress Escherichia coli Exopolysaccharides Genes Genetic engineering Genomes Genomics Growth media Heavy metals Hostages Iron Life Sciences Media (culture) Metals Metals - toxicity Microbiology and Parasitology Microscopy, Electron, Scanning Mineralization Mutagenesis Mutants Mutation - genetics Physiological aspects Polysaccharides - pharmacology Salts Sedimentation Sequence Deletion Sodium chloride Sodium Chloride - toxicity Spectroscopy, Fourier Transform Infrared Starvation Stress, Physiological - drug effects Stresses Synechocystis Synechocystis - drug effects Synechocystis - growth & development Synechocystis - metabolism |
title | Multidisciplinary evidences that Synechocystis PCC6803 exopolysaccharides operate in cell sedimentation and protection against salt and metal stresses |
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