Epstein-Barr virus Zta upregulates matrix metalloproteinases 3 and 9 that synergistically promote cell invasion in vitro

• Published in: PloS one 2013-02, Vol.8 (2), p.e56121-e56121
• Main Authors: Lan, Yu-Yan, Yeh, Tzu-Hao, Lin, Wei-Hung, Wu, Shih-Yi, Lai, Hsiao-Ching, Chang, Fang-Hsin, Takada, Kenzo, Chang, Yao
• Format: Article
• Language: English
• Subjects: Antigens; Binding; Binding sites; Biology; Breast cancer; Carcinoma; Cell adhesion & migration; Cell culture; Cell Line, Tumor; Cell migration; Cell Movement; Deoxyribonucleic acid; DNA; E2F Transcription Factors - metabolism; Epithelial cells; Epithelial Cells - metabolism; Epithelial Cells - pathology; Epithelial Cells - virology; Epstein-Barr virus; Gelatinase B; Gene expression; Genomes; Herpesvirus 4, Human - physiology; Hospitals; Humans; Immunology; Infections; Infectious diseases; Invasiveness; Matrix metalloproteinase; Matrix Metalloproteinase 3 - genetics; Matrix Metalloproteinase 9 - genetics; Matrix metalloproteinases; Medical prognosis; Medical research; Medicine; Metalloproteinase; Metastasis; Motility; mRNA; Nasopharyngeal Carcinoma; Nasopharyngeal Neoplasms - pathology; Neoplasm Invasiveness; Promoter Regions, Genetic - genetics; Proteins; RNA; Stromelysin 1; Studies; Throat cancer; Trans-Activators - metabolism; Transcription factors; Transcriptional Activation; Tumors; Up-Regulation; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0056121

Zta is a lytic transactivator of Epstein-Barr virus (EBV) and has been shown to promote migration and invasion of epithelial cells. Although previous studies indicate that Zta induces expression of matrix metalloproteinase (MMP) 9 and MMP1, direct evidence linking the MMPs to Zta-induced cell migration and invasion is still lacking. Here we performed a series of in vitro studies to re-examine the expression profile and biologic functions of Zta-induced MMPs in epithelial cells derived from nasopharyngeal carcinoma. We found that, in addition to MMP9, MMP3 was a new target gene upregulated by Zta. Ectopic Zta expression in EBV-negative cells increased both mRNA and protein production of MMP3. Endogenous Zta also contributed to induction of MMP3 expression, migration and invasion of EBV-infected cells. Zta activated the MMP3 promoter through three AP-1 elements, and its DNA-binding domain was required for the promoter binding and MMP3 induction. We further tested the effects of MMP3 and MMP9 on cell motility and invasiveness in vitro. Zta-promoted cell migration required MMP3 but not MMP9. On the other hand, both MMP3 and MMP9 were essential for Zta-induced cell invasion, and co-expression of the two MMPs synergistically increased cell invasiveness. Therefore, this study provides integrated evidence demonstrating that, at least in the in vitro cell models, Zta drives cell migration and invasion through MMPs.