Orientations and proximities of the extracellular ends of transmembrane helices S0 and S4 in open and closed BK potassium channels

• Published in: PloS one 2013-03, Vol.8 (3), p.e58335
• Main Authors: Niu, Xiaowei, Liu, Guoxia, Wu, Roland S, Chudasama, Neelesh, Zakharov, Sergey I, Karlin, Arthur, Marx, Steven O
• Format: Article
• Language: English
• Subjects: Amino Acid Substitution; Ammonium; Animals; Biochemistry; Biology; Biotinylation; Cardiology; Cell Membrane - metabolism; Cell surface; Conductance; Crosslinking; Cysteine - chemistry; Deactivation; Digital television; Disulfides - chemistry; Dithiothreitol; Electric potential; HEK293 Cells; Helices; Humans; Immobilization; Large-Conductance Calcium-Activated Potassium Channels - chemistry; Medicine; Membrane Potentials; Mice; Mutation; Oxygen - chemistry; Physiology; Polymer crosslinking; Potassium; Potassium channels; Potassium conductance; Protein Structure, Secondary; Rate constants; Reoxidation; Residues; Resistance; Sensors; Smooth muscle; Surgeons; Voltage
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0058335

The large-conductance potassium channel (BK) α subunit contains a transmembrane (TM) helix S0 preceding the canonical TM helices S1 through S6. S0 lies between S4 and the TM2 helix of the regulatory β1 subunit. Pairs of Cys were substituted in the first helical turns in the membrane of BK α S0 and S4 and in β1 TM2. One such pair, W22C in S0 and W203C in S4, was 95% crosslinked endogenously. Under voltage-clamp conditions in outside-out patches, this crosslink was reduced by DTT and reoxidized by a membrane-impermeant bis-quaternary ammonium derivative of diamide. The rate constants for this reoxidation were not significantly different in the open and closed states of the channel. Thus, these two residues are approximately equally close in the two states. In addition, 90% crosslinking of a second pair, R20C in S0 and W203C in S4, had no effect on the V50 for opening. Taken together, these findings indicate that separation between residues at the extracellular ends of S0 and S4 is not required for voltage-sensor activation. On the contrary, even though W22C and W203C were equally likely to form a disulfide in the activated and deactivated states, relative immobilization by crosslinking of these two residues favored the activated state. Furthermore, the efficiency of recrosslinking of W22C and W203C on the cell surface was greater in the presence of the β1 subunit than in its absence, consistent with β1 acting through S0 to stabilize its immobilization relative to α S4.