Metabolomics reveals metabolic targets and biphasic responses in breast cancer cells treated by curcumin alone and in association with docetaxel

• Published in: PloS one 2013-03, Vol.8 (3), p.e57971
• Main Authors: Bayet-Robert, Mathilde, Morvan, Daniel
• Format: Article
• Language: English
• Subjects: Anti-inflammatory agents; Antineoplastic Agents - pharmacology; Apoptosis; Biology; Biomarkers; Biosynthesis; Breast cancer; Breast Neoplasms - metabolism; Cancer; Cancer cells; Cancer therapies; Cell culture; Cell Line, Tumor; Chemistry; Comet Assay; Curcumin; Curcumin - pharmacology; Disease; Docetaxel; Drug dosages; Enzymes; Ethanolamine; Fatty acids; Female; Gene expression; Glucose metabolism; Glutathione - metabolism; Glutathione transferase; Glutathione Transferase - metabolism; Humans; Inflammation; Kinases; Lipid metabolism; Lipid peroxidation; Lipids; Magnetic Resonance Spectroscopy; MCF-7 Cells; Medicine; Metabolism; Metabolites; Metabolomics; Multivariate Analysis; NMR spectroscopy; Nuclear magnetic resonance spectroscopy; Oxidative Stress; Physiological aspects; Proteins; Rodents; Spectroscopy; Statistical analysis; Taurine; Taxoids - pharmacology; Transcription factors; Treatment Outcome; Tumor cells
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0057971

Curcumin (CUR) has deserved extensive research due to its anti-inflammatory properties, of interest in human diseases including cancer. However, pleiotropic even paradoxical responses of tumor cells have been reported, and the mechanisms of action of CUR remain uncompletely elucidated. (1)H-NMR spectroscopy-based metabolomics was applied to get novel insight into responses of MCF7 and MDA-MB-231 breast cancer cells to CUR alone, and MCF7 cells to CUR in cotreatment with docetaxel (DTX). In both cell types, a major target of CUR was glutathione metabolism. Total glutathione (GSx) increased at low dose CUR (≤ 10 mg.l(-1)-28 µM-) (up to +121% in MCF7 cells, P