HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2

HOXC9 belongs to the family of homeobox transcription factors, which are regulators of body patterning and development. HOXC9 acts as a negative regulator on blood endothelial cells but its function on lymphatic vessel development has not been studied. The hyaluronan receptor homologs stabilin 1 and...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-03, Vol.8 (3), p.e58311-e58311
Main Authors: Stoll, Sandra J, Bartsch, Susanne, Kroll, Jens
Format: Article
Language:English
Subjects:
Angiogenesis
Animals
Assembly
Biology
Blotting, Western
Cell adhesion & migration
Cell Adhesion Molecules, Neuronal - metabolism
Cell migration
Cytokines
Danio rerio
DNA Primers - genetics
Embryonic development
Embryos
Endothelial cells
Endothelial Cells - metabolism
Endothelium
Ethics
Gene Expression Regulation, Developmental - physiology
Genes
Green Fluorescent Proteins - metabolism
Homeobox
Homeodomain Proteins - metabolism
Homology
Human Umbilical Vein Endothelial Cells
Humans
Hyaluronic acid
Immunoglobulins
Lymphatic system
Lymphatic Vessels - embryology
Mathematics
Medical research
Medical technology
Medicine
Metastasis
Microscopy, Fluorescence
Morphogenesis
Pattern formation
Receptor mechanisms
Receptors
Receptors, Lymphocyte Homing - metabolism
Regulators
Reverse Transcriptase Polymerase Chain Reaction
RNA
Rodents
Studies
Thoracic duct
Thoracic Duct - embryology
Transcription factors
Transfection
Veins & arteries
Vertebrates
Zebrafish
Zebrafish - embryology
Zebrafish Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c692t-9e374ef411d043ecea9f37bfb7885eb3b2e0f24d102bfe7d3bd7e08a5d5f1ad3
cites cdi_FETCH-LOGICAL-c692t-9e374ef411d043ecea9f37bfb7885eb3b2e0f24d102bfe7d3bd7e08a5d5f1ad3
container_end_page e58311
container_issue 3
container_start_page e58311
container_title PloS one
container_volume 8
creator Stoll, Sandra J
Bartsch, Susanne
Kroll, Jens
description HOXC9 belongs to the family of homeobox transcription factors, which are regulators of body patterning and development. HOXC9 acts as a negative regulator on blood endothelial cells but its function on lymphatic vessel development has not been studied. The hyaluronan receptor homologs stabilin 1 and stabilin 2 are expressed in endothelial cells but their role in vascular development is poorly understood. This study was aimed at investigating the function of HOXC9, stabilin 2 and stabilin 1 in lymphatic vessel development in zebrafish and in endothelial cells. Morpholino-based expression silencing of HOXC9 repressed parachordal lymphangioblast assembly and thoracic duct formation in zebrafish. HOXC9 positively regulated stabilin 2 expression in zebrafish and in HUVECs and expression silencing of stabilin 2 phenocopied the HOXC9 morphant vascular phenotype. This effect could be compensated by HOXC9 mRNA injection in stabilin 2 morphant zebrafish embryos. Stabilin 1 also regulated parachordal lymphangioblast and thoracic duct formation in zebrafish but acts independently of HOXC9. On a cellular level stabilin 1 and stabilin 2 regulated endothelial cell migration and in-gel sprouting angiogenesis in endothelial cells. HOXC9 was identified as novel transcriptional regulator of parachordal lymphangioblast assembly and thoracic duct formation in zebrafish that acts via stabilin 2. Stabilin 1, which acts independently of HOXC9, has a similar function in zebrafish and both receptors control important cellular processes in endothelial cells.
doi_str_mv 10.1371/journal.pone.0058311
format article
fullrecord <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1330881292</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478411023</galeid><doaj_id>oai_doaj_org_article_0e515098d3454475aed4f85a3af0975d</doaj_id><sourcerecordid>A478411023</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-9e374ef411d043ecea9f37bfb7885eb3b2e0f24d102bfe7d3bd7e08a5d5f1ad3</originalsourceid><addsrcrecordid>eNqNk1GLEzEQxxdRvPP0G4guCKIPrckm6e6-CEdRr3BQ0EN8C7ObSTc13fSS7OH56U3t3tHKPUgICZPf_CczyWTZS0qmlJX0w9oNvgc73boep4SIilH6KDulNSsms4Kwxwf7k-xZCOsEsWo2e5qdFIxXnFB-mv28WP6Y17nH1WAhYsi18xuIxvW50_kWPLSd8wpsbm832w76lXFbCyGGHHqVxw7TdIkyba6GNuamz39j40Gb0OU3BvIQoTE2mYvn2RMNNuCLcT3Lrj5_uppfTC6XXxbz88tJO6uLOKmRlRw1p1QRzrBFqDUrG92UVSWwYU2BRBdcUVI0GkvFGlUiqUAooSkodpa93sturQtyLFOQlDFSVbSoi0Qs9oRysJZbbzbgb6UDI_8anF9J8NG0FiVBQQWpK8W44LwUgIrrSgADTepS7KJ9HKMNzQZVi330YI9Ej09608mVu5FM1OkFRBJ4Nwp4dz1giHJjQovWQo9u2N2blpyIouIJffMP-nB2I7WClIDptUtx252oPOdllepKCpao6QNUGgo3pk1_SptkP3J4f-SQmIi_4gqGEOTi29f_Z5ffj9m3B2yHYGMXnB12fzAcg3wPtt6F4FHfF5kSuWuJu2rIXUvIsSWS26vDB7p3uusB9gcHawcB</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1330881292</pqid></control><display><type>article</type><title>HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2</title><source>Publicly Available Content Database</source><source>PubMed Central</source><creator>Stoll, Sandra J ; Bartsch, Susanne ; Kroll, Jens</creator><contributor>Kume, Tsutomu</contributor><creatorcontrib>Stoll, Sandra J ; Bartsch, Susanne ; Kroll, Jens ; Kume, Tsutomu</creatorcontrib><description>HOXC9 belongs to the family of homeobox transcription factors, which are regulators of body patterning and development. HOXC9 acts as a negative regulator on blood endothelial cells but its function on lymphatic vessel development has not been studied. The hyaluronan receptor homologs stabilin 1 and stabilin 2 are expressed in endothelial cells but their role in vascular development is poorly understood. This study was aimed at investigating the function of HOXC9, stabilin 2 and stabilin 1 in lymphatic vessel development in zebrafish and in endothelial cells. Morpholino-based expression silencing of HOXC9 repressed parachordal lymphangioblast assembly and thoracic duct formation in zebrafish. HOXC9 positively regulated stabilin 2 expression in zebrafish and in HUVECs and expression silencing of stabilin 2 phenocopied the HOXC9 morphant vascular phenotype. This effect could be compensated by HOXC9 mRNA injection in stabilin 2 morphant zebrafish embryos. Stabilin 1 also regulated parachordal lymphangioblast and thoracic duct formation in zebrafish but acts independently of HOXC9. On a cellular level stabilin 1 and stabilin 2 regulated endothelial cell migration and in-gel sprouting angiogenesis in endothelial cells. HOXC9 was identified as novel transcriptional regulator of parachordal lymphangioblast assembly and thoracic duct formation in zebrafish that acts via stabilin 2. Stabilin 1, which acts independently of HOXC9, has a similar function in zebrafish and both receptors control important cellular processes in endothelial cells.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0058311</identifier><identifier>PMID: 23484014</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Angiogenesis ; Animals ; Assembly ; Biology ; Blotting, Western ; Cell adhesion &amp; migration ; Cell Adhesion Molecules, Neuronal - metabolism ; Cell migration ; Cytokines ; Danio rerio ; DNA Primers - genetics ; Embryonic development ; Embryos ; Endothelial cells ; Endothelial Cells - metabolism ; Endothelium ; Ethics ; Gene Expression Regulation, Developmental - physiology ; Genes ; Green Fluorescent Proteins - metabolism ; Homeobox ; Homeodomain Proteins - metabolism ; Homology ; Human Umbilical Vein Endothelial Cells ; Humans ; Hyaluronic acid ; Immunoglobulins ; Lymphatic system ; Lymphatic Vessels - embryology ; Mathematics ; Medical research ; Medical technology ; Medicine ; Metastasis ; Microscopy, Fluorescence ; Morphogenesis ; Pattern formation ; Receptor mechanisms ; Receptors ; Receptors, Lymphocyte Homing - metabolism ; Regulators ; Reverse Transcriptase Polymerase Chain Reaction ; RNA ; Rodents ; Studies ; Thoracic duct ; Thoracic Duct - embryology ; Transcription factors ; Transfection ; Veins &amp; arteries ; Vertebrates ; Zebrafish ; Zebrafish - embryology ; Zebrafish Proteins - metabolism</subject><ispartof>PloS one, 2013-03, Vol.8 (3), p.e58311-e58311</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Stoll et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Stoll et al 2013 Stoll et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-9e374ef411d043ecea9f37bfb7885eb3b2e0f24d102bfe7d3bd7e08a5d5f1ad3</citedby><cites>FETCH-LOGICAL-c692t-9e374ef411d043ecea9f37bfb7885eb3b2e0f24d102bfe7d3bd7e08a5d5f1ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1330881292/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1330881292?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23484014$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kume, Tsutomu</contributor><creatorcontrib>Stoll, Sandra J</creatorcontrib><creatorcontrib>Bartsch, Susanne</creatorcontrib><creatorcontrib>Kroll, Jens</creatorcontrib><title>HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>HOXC9 belongs to the family of homeobox transcription factors, which are regulators of body patterning and development. HOXC9 acts as a negative regulator on blood endothelial cells but its function on lymphatic vessel development has not been studied. The hyaluronan receptor homologs stabilin 1 and stabilin 2 are expressed in endothelial cells but their role in vascular development is poorly understood. This study was aimed at investigating the function of HOXC9, stabilin 2 and stabilin 1 in lymphatic vessel development in zebrafish and in endothelial cells. Morpholino-based expression silencing of HOXC9 repressed parachordal lymphangioblast assembly and thoracic duct formation in zebrafish. HOXC9 positively regulated stabilin 2 expression in zebrafish and in HUVECs and expression silencing of stabilin 2 phenocopied the HOXC9 morphant vascular phenotype. This effect could be compensated by HOXC9 mRNA injection in stabilin 2 morphant zebrafish embryos. Stabilin 1 also regulated parachordal lymphangioblast and thoracic duct formation in zebrafish but acts independently of HOXC9. On a cellular level stabilin 1 and stabilin 2 regulated endothelial cell migration and in-gel sprouting angiogenesis in endothelial cells. HOXC9 was identified as novel transcriptional regulator of parachordal lymphangioblast assembly and thoracic duct formation in zebrafish that acts via stabilin 2. Stabilin 1, which acts independently of HOXC9, has a similar function in zebrafish and both receptors control important cellular processes in endothelial cells.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Assembly</subject><subject>Biology</subject><subject>Blotting, Western</subject><subject>Cell adhesion &amp; migration</subject><subject>Cell Adhesion Molecules, Neuronal - metabolism</subject><subject>Cell migration</subject><subject>Cytokines</subject><subject>Danio rerio</subject><subject>DNA Primers - genetics</subject><subject>Embryonic development</subject><subject>Embryos</subject><subject>Endothelial cells</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelium</subject><subject>Ethics</subject><subject>Gene Expression Regulation, Developmental - physiology</subject><subject>Genes</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Homeobox</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Homology</subject><subject>Human Umbilical Vein Endothelial Cells</subject><subject>Humans</subject><subject>Hyaluronic acid</subject><subject>Immunoglobulins</subject><subject>Lymphatic system</subject><subject>Lymphatic Vessels - embryology</subject><subject>Mathematics</subject><subject>Medical research</subject><subject>Medical technology</subject><subject>Medicine</subject><subject>Metastasis</subject><subject>Microscopy, Fluorescence</subject><subject>Morphogenesis</subject><subject>Pattern formation</subject><subject>Receptor mechanisms</subject><subject>Receptors</subject><subject>Receptors, Lymphocyte Homing - metabolism</subject><subject>Regulators</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA</subject><subject>Rodents</subject><subject>Studies</subject><subject>Thoracic duct</subject><subject>Thoracic Duct - embryology</subject><subject>Transcription factors</subject><subject>Transfection</subject><subject>Veins &amp; arteries</subject><subject>Vertebrates</subject><subject>Zebrafish</subject><subject>Zebrafish - embryology</subject><subject>Zebrafish Proteins - metabolism</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1GLEzEQxxdRvPP0G4guCKIPrckm6e6-CEdRr3BQ0EN8C7ObSTc13fSS7OH56U3t3tHKPUgICZPf_CczyWTZS0qmlJX0w9oNvgc73boep4SIilH6KDulNSsms4Kwxwf7k-xZCOsEsWo2e5qdFIxXnFB-mv28WP6Y17nH1WAhYsi18xuIxvW50_kWPLSd8wpsbm832w76lXFbCyGGHHqVxw7TdIkyba6GNuamz39j40Gb0OU3BvIQoTE2mYvn2RMNNuCLcT3Lrj5_uppfTC6XXxbz88tJO6uLOKmRlRw1p1QRzrBFqDUrG92UVSWwYU2BRBdcUVI0GkvFGlUiqUAooSkodpa93sturQtyLFOQlDFSVbSoi0Qs9oRysJZbbzbgb6UDI_8anF9J8NG0FiVBQQWpK8W44LwUgIrrSgADTepS7KJ9HKMNzQZVi330YI9Ej09608mVu5FM1OkFRBJ4Nwp4dz1giHJjQovWQo9u2N2blpyIouIJffMP-nB2I7WClIDptUtx252oPOdllepKCpao6QNUGgo3pk1_SptkP3J4f-SQmIi_4gqGEOTi29f_Z5ffj9m3B2yHYGMXnB12fzAcg3wPtt6F4FHfF5kSuWuJu2rIXUvIsSWS26vDB7p3uusB9gcHawcB</recordid><startdate>20130306</startdate><enddate>20130306</enddate><creator>Stoll, Sandra J</creator><creator>Bartsch, Susanne</creator><creator>Kroll, Jens</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130306</creationdate><title>HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2</title><author>Stoll, Sandra J ; Bartsch, Susanne ; Kroll, Jens</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-9e374ef411d043ecea9f37bfb7885eb3b2e0f24d102bfe7d3bd7e08a5d5f1ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Assembly</topic><topic>Biology</topic><topic>Blotting, Western</topic><topic>Cell adhesion &amp; migration</topic><topic>Cell Adhesion Molecules, Neuronal - metabolism</topic><topic>Cell migration</topic><topic>Cytokines</topic><topic>Danio rerio</topic><topic>DNA Primers - genetics</topic><topic>Embryonic development</topic><topic>Embryos</topic><topic>Endothelial cells</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelium</topic><topic>Ethics</topic><topic>Gene Expression Regulation, Developmental - physiology</topic><topic>Genes</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Homeobox</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Homology</topic><topic>Human Umbilical Vein Endothelial Cells</topic><topic>Humans</topic><topic>Hyaluronic acid</topic><topic>Immunoglobulins</topic><topic>Lymphatic system</topic><topic>Lymphatic Vessels - embryology</topic><topic>Mathematics</topic><topic>Medical research</topic><topic>Medical technology</topic><topic>Medicine</topic><topic>Metastasis</topic><topic>Microscopy, Fluorescence</topic><topic>Morphogenesis</topic><topic>Pattern formation</topic><topic>Receptor mechanisms</topic><topic>Receptors</topic><topic>Receptors, Lymphocyte Homing - metabolism</topic><topic>Regulators</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA</topic><topic>Rodents</topic><topic>Studies</topic><topic>Thoracic duct</topic><topic>Thoracic Duct - embryology</topic><topic>Transcription factors</topic><topic>Transfection</topic><topic>Veins &amp; arteries</topic><topic>Vertebrates</topic><topic>Zebrafish</topic><topic>Zebrafish - embryology</topic><topic>Zebrafish Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Stoll, Sandra J</creatorcontrib><creatorcontrib>Bartsch, Susanne</creatorcontrib><creatorcontrib>Kroll, Jens</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Opposing Viewpoints Resource Center</collection><collection>Science In Context</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>ProQuest Nursing &amp; Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological &amp; Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>Advanced Technologies &amp; Aerospace Database‎ (1962 - current)</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Materials Science Database</collection><collection>Nursing &amp; Allied Health Database (Alumni Edition)</collection><collection>Meteorological &amp; Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>Biological Sciences</collection><collection>Agriculture Science Database</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest Engineering Database</collection><collection>Nursing &amp; Allied Health Premium</collection><collection>ProQuest advanced technologies &amp; aerospace journals</collection><collection>ProQuest Advanced Technologies &amp; Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Stoll, Sandra J</au><au>Bartsch, Susanne</au><au>Kroll, Jens</au><au>Kume, Tsutomu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-03-06</date><risdate>2013</risdate><volume>8</volume><issue>3</issue><spage>e58311</spage><epage>e58311</epage><pages>e58311-e58311</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>HOXC9 belongs to the family of homeobox transcription factors, which are regulators of body patterning and development. HOXC9 acts as a negative regulator on blood endothelial cells but its function on lymphatic vessel development has not been studied. The hyaluronan receptor homologs stabilin 1 and stabilin 2 are expressed in endothelial cells but their role in vascular development is poorly understood. This study was aimed at investigating the function of HOXC9, stabilin 2 and stabilin 1 in lymphatic vessel development in zebrafish and in endothelial cells. Morpholino-based expression silencing of HOXC9 repressed parachordal lymphangioblast assembly and thoracic duct formation in zebrafish. HOXC9 positively regulated stabilin 2 expression in zebrafish and in HUVECs and expression silencing of stabilin 2 phenocopied the HOXC9 morphant vascular phenotype. This effect could be compensated by HOXC9 mRNA injection in stabilin 2 morphant zebrafish embryos. Stabilin 1 also regulated parachordal lymphangioblast and thoracic duct formation in zebrafish but acts independently of HOXC9. On a cellular level stabilin 1 and stabilin 2 regulated endothelial cell migration and in-gel sprouting angiogenesis in endothelial cells. HOXC9 was identified as novel transcriptional regulator of parachordal lymphangioblast assembly and thoracic duct formation in zebrafish that acts via stabilin 2. Stabilin 1, which acts independently of HOXC9, has a similar function in zebrafish and both receptors control important cellular processes in endothelial cells.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23484014</pmid><doi>10.1371/journal.pone.0058311</doi><tpages>e58311</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1932-6203
ispartof PloS one, 2013-03, Vol.8 (3), p.e58311-e58311
issn 1932-6203
1932-6203
language eng
recordid cdi_plos_journals_1330881292
source Publicly Available Content Database; PubMed Central
subjects Angiogenesis
Animals
Assembly
Biology
Blotting, Western
Cell adhesion & migration
Cell Adhesion Molecules, Neuronal - metabolism
Cell migration
Cytokines
Danio rerio
DNA Primers - genetics
Embryonic development
Embryos
Endothelial cells
Endothelial Cells - metabolism
Endothelium
Ethics
Gene Expression Regulation, Developmental - physiology
Genes
Green Fluorescent Proteins - metabolism
Homeobox
Homeodomain Proteins - metabolism
Homology
Human Umbilical Vein Endothelial Cells
Humans
Hyaluronic acid
Immunoglobulins
Lymphatic system
Lymphatic Vessels - embryology
Mathematics
Medical research
Medical technology
Medicine
Metastasis
Microscopy, Fluorescence
Morphogenesis
Pattern formation
Receptor mechanisms
Receptors
Receptors, Lymphocyte Homing - metabolism
Regulators
Reverse Transcriptase Polymerase Chain Reaction
RNA
Rodents
Studies
Thoracic duct
Thoracic Duct - embryology
Transcription factors
Transfection
Veins & arteries
Vertebrates
Zebrafish
Zebrafish - embryology
Zebrafish Proteins - metabolism
title HOXC9 regulates formation of parachordal lymphangioplasts and the thoracic duct in zebrafish via stabilin 2
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T21%3A12%3A56IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=HOXC9%20regulates%20formation%20of%20parachordal%20lymphangioplasts%20and%20the%20thoracic%20duct%20in%20zebrafish%20via%20stabilin%202&rft.jtitle=PloS%20one&rft.au=Stoll,%20Sandra%20J&rft.date=2013-03-06&rft.volume=8&rft.issue=3&rft.spage=e58311&rft.epage=e58311&rft.pages=e58311-e58311&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0058311&rft_dat=%3Cgale_plos_%3EA478411023%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-9e374ef411d043ecea9f37bfb7885eb3b2e0f24d102bfe7d3bd7e08a5d5f1ad3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1330881292&rft_id=info:pmid/23484014&rft_galeid=A478411023&rfr_iscdi=true