Aerobactin synthesis genes iucA and iucC contribute to the pathogenicity of avian pathogenic Escherichia coli O2 strain E058

Aerobactin genes are known to be present in virulent strains and absent from avirulent strains, but contributions of iucC and iucA, which are involved in aerobactin synthesis, to the pathogenicity of avian pathogenic Escherichia coli (APEC) have not been clarified. In this study, effects of double m...

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Bibliographic Details
Published in:PloS one 2013-02, Vol.8 (2), p.e57794
Main Authors: Ling, Jielu, Pan, Haizhu, Gao, Qingqing, Xiong, Liping, Zhou, Yefei, Zhang, Debao, Gao, Song, Liu, Xiufan
Format: Article
Language:English
Subjects:
Aerobactin
Animals
Biology
Cell survival
Chickens - blood
Chickens - microbiology
Colonies
Colonization
Colony Count, Microbial
Deoxyribonucleic acid
DNA
E coli
Escherichia coli
Escherichia coli - genetics
Escherichia coli - growth & development
Escherichia coli - pathogenicity
Escherichia coli Infections - genetics
Escherichia coli Infections - microbiology
Escherichia coli Infections - veterinary
Gene Deletion
Gene Expression Regulation, Bacterial
Genes
Genes, Bacterial - genetics
Genetic Complementation Test
Heart
Hydroxamic Acids - metabolism
Infectious diseases
Ingestion
Kidneys
Laboratory animals
Liver
Lungs
Mutants
Mutation
Organs
Pathogenicity
Pathogens
Plasmids
Poultry
Poultry Diseases - microbiology
Shigella
Specific Pathogen-Free Organisms
Spleen
Synthesis
Veterinary colleges
Veterinary medicine
Veterinary Science
Virulence
Virulence - genetics
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Summary:Aerobactin genes are known to be present in virulent strains and absent from avirulent strains, but contributions of iucC and iucA, which are involved in aerobactin synthesis, to the pathogenicity of avian pathogenic Escherichia coli (APEC) have not been clarified. In this study, effects of double mutants (iucA/iutA or iucC/iutA) compared to those of single mutants (iucA, iucC or iutA) of aerobactin genes on the virulence of APEC strain E058 were examined both in vitro (aerobactin production, ingestion into HD-11 cells, survival in chicken serum) and in vivo (competitive growth against parental strain, colonization and persistence). In competitive co-infection assays, compared to the E058 parental strain, the E058ΔiucA mutant was significantly reduced in the liver, kidney, spleen (all P
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0057794