Cyanobactericidal effect of Streptomyces sp. HJC-D1 on Microcystis auruginosa

• Published in: PloS one 2013-02, Vol.8 (2), p.e57654-e57654
• Main Authors: Kong, Yun, Xu, Xiangyang, Zhu, Liang
• Format: Article
• Language: English
• Subjects: Algae; Antioxidants; Biology; Breakage; Carbohydrate Metabolism; Catalase; Catalase - metabolism; Cell culture; Cell death; Cellulose acetate; Chlorella vulgaris; Cyanobacteria; Cytometry; Electron microscopy; Environmental engineering; Enzymatic activity; Enzyme activity; Enzymes; Eutrophication; Exposure; Gene expression; Lipid peroxidation; Malondialdehyde; Malondialdehyde - metabolism; Mathematics; Microbial Viability; Microcystis; Microcystis - enzymology; Microcystis - growth & development; Microcystis - ultrastructure; Microcystis aeruginosa; Microorganisms; Oxygen; Peroxidase; Peroxidase - metabolism; Physiology; Plankton; Reactive oxygen species; Reactive Oxygen Species - metabolism; Streptomyces; Streptomyces - physiology; Subcellular Fractions - metabolism; Subcellular Fractions - ultrastructure; Superoxide dismutase; Superoxide Dismutase - metabolism; Transmission electron microscopy
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0057654

An isolated strain Streptomyces sp. HJC-D1 was applied to inhibit the growth of cyanobacterium Microcystis aeruginosa FACHB-905. The effect of Streptomyces sp. HJC-D1 culture broth on the cell integrity and physiological characteristics of M. aeruginosa FACHB-905 was investigated using the flow cytometry (FCM), enzyme activity and transmission electron microscopy (TEM) methods. Results showed that the growth of M. aeruginosa FACHB-905 was significantly inhibited, and the percentage of live cells depended on the culture broth concentration and exposure time. The activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) increased with exposure concentration and exposure time, and the significant increase of reactive oxygen species (ROS) led to the disruption of the subcellular structure of M. aeruginosa FACHB-905, and caused the increase of malondialdehyde (MDA). Furthermore, TEM observation suggested the presence of three stages (cell breakage, organelle release and cell death) for the cyanobactericidal process of Streptomyces sp. HJC-D1. Therefore, Streptomyces sp. HJC-D1 not only affected antioxidant enzyme activities and ROS level, but also destroyed the subcellular structure of M. aeruginosa FACHB-905, demonstrating excellent cyanobactericidal properties.