Regulation of spine density and morphology by IQGAP1 protein domains

IQGAP1 is a scaffolding protein that regulates spine number. We now show a differential role for IQGAP1 domains in spine morphogenesis, in which a region of the N-terminus that promotes Arp2/3-mediated actin polymerization and branching stimulates spine head formation while a region that binds to Cd...

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Bibliographic Details
Published in:PloS one 2013-02, Vol.8 (2), p.e56574-e56574
Main Authors: Jausoro, Ignacio, Mestres, Ivan, Quassollo, Gonzalo, Masseroni, Lujan, Heredia, Florencia, Caceres, Alfredo
Format: Article
Language:English
Subjects:
Actin
Animals
Biology
Cdc42 protein
Dendritic Spines - metabolism
Female
G proteins
Gene Expression
Hippocampus - cytology
IQGAP1 protein
Kinases
Laboratories
Medicine
Microscopy
Morphogenesis
Morphology
Muscle proteins
Mutation
N-Terminus
Neurobiology
Neurons
Neurons - cytology
Neurons - metabolism
Neurosciences
Polymerization
Pregnancy
Protein Interaction Domains and Motifs
Proteins
ras GTPase-Activating Proteins - chemistry
ras GTPase-Activating Proteins - genetics
ras GTPase-Activating Proteins - metabolism
Rats
Receptors, N-Methyl-D-Aspartate - metabolism
Scaffolding
Spine
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Summary:IQGAP1 is a scaffolding protein that regulates spine number. We now show a differential role for IQGAP1 domains in spine morphogenesis, in which a region of the N-terminus that promotes Arp2/3-mediated actin polymerization and branching stimulates spine head formation while a region that binds to Cdc42 and Rac is required for stalk extension. Conversely, IQGAP1 rescues spine deficiency induced by expression of dominant negative Cdc42 by stimulating formation of stubby spines. Together, our observations place IQGAP1 as a crucial regulator of spine number and shape acting through the N-Wasp Arp2/3 complex, as well as upstream and downstream of Cdc42.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0056574