Sequential soil transport and its influence on the spatial organisation of collective digging in leaf-cutting ants

• Published in: PloS one 2013-02, Vol.8 (2), p.e57040-e57040
• Main Authors: Pielström, Steffen, Roces, Flavio
• Format: Article
• Language: English
• Subjects: Animal behavior; Animals; Ants; Ants - physiology; Atta sexdens; Atta vollenweideri; Biology; Body Size; Carriers; Choice Behavior; Cues; Deposition; Excavating machinery; Excavation; Excavators; Foraging behavior; Formicidae; Fungi; Hymenoptera; International economic relations; Isoptera; Leaves; Load; Motion; Nest building; Nesting Behavior; Nests; Pellets; Physiology; Plant Leaves; Soil; Soil investigations; Soils; Spatial Analysis; Telecommunications industry; Time Factors; Transport; Ventilation; Veterinary Science; Workers (insect caste)
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0057040

The Chaco leaf-cutting ant Atta vollenweideri (Forel) inhabits large and deep subterranean nests composed of a large number of fungus and refuse chambers. The ants dispose of the excavated soil by forming small pellets that are carried to the surface. For ants in general, the organisation of underground soil transport during nest building remains completely unknown. In the laboratory, we investigated how soil pellets are formed and transported, and whether their occurrence influences the spatial organisation of collective digging. Similar to leaf transport, we discovered size matching between soil pellet mass and carrier mass. Workers observed while digging excavated pellets at a rate of 26 per hour. Each excavator deposited its pellets in an individual cluster, independently of the preferred deposition sites of other excavators. Soil pellets were transported sequentially over 2 m, and the transport involved up to 12 workers belonging to three functionally distinct groups: excavators, several short-distance carriers that dropped the collected pellets after a few centimetres, and long-distance, last carriers that reached the final deposition site. When initiating a new excavation, the proportion of long-distance carriers increased from 18% to 45% within the first five hours, and remained unchanged over more than 20 hours. Accumulated, freshly-excavated pellets significantly influenced the workers' decision where to start digging in a choice experiment. Thus, pellets temporarily accumulated as a result of their sequential transport provide cues that spatially organise collective nest excavation.