Possible natural hybridization of two morphologically distinct species of Acropora (Cnidaria, Scleractinia) in the Pacific: fertilization and larval survival rates

• Published in: PloS one 2013-02, Vol.8 (2), p.e56701-e56701
• Main Authors: Isomura, Naoko, Iwao, Kenji, Fukami, Hironobu
• Format: Article
• Language: English
• Subjects: Acropora; Acropora intermedia; Analysis; Animal reproduction; Animals; Biology; Cnidaria; Cnidaria - embryology; Cnidaria - genetics; Cnidaria - growth & development; Cnidaria - physiology; Colonies; Coral reefs; Corals; Eggs; Embryonic development; Embryos; Evolution; Experiments; Fertilization; Fertilization - genetics; Galaxea; Hybridization; Hybridization, Genetic; Hybrids; Hypotheses; Interspecific; Laboratories; Larva - genetics; Larva - growth & development; Larvae; Montastraea annularis; Morphology; Pacific Ocean; Science; Scleractinia; Species; Species Specificity; Sperm; Studies; Survival
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0056701

Natural hybridization of corals in the Indo-Pacific has been considered rather rare. However, field studies have observed many corals with intermediate interspecific or unusual morphologies. Given that the existence of F1 hybrids with intermediate interspecific morphologies has been proven in the Caribbean, hybrids may also inhabit the Indo-Pacific and occur more frequently than expected. In this study, we focused on two morphologically different species, Acropora florida and A. intermedia, and performed crossing experiments at Akajima Island, Japan. Results showed that these species could hybridize in both directions via eggs and sperm, but that fertilization rates significantly differed according to which species provided eggs. These results are similar to those reported from the Caribbean. Although all embryos developed normally to the planular larval stage, the developmental processes of some hybrid embryos were delayed by approximately 1 h compared with conspecific embryos, suggesting that fertilization occurred 1 h later in interspecific crosses than in intraspecific crosses. More successful hybridization could occur under conditions with low numbers of conspecific colonies. Additionally, a comparison of survival rates between hybrid and intraspecific larvae revealed that intra- and interspecific larvae produced from eggs of A. florida survived for significantly longer than those produced from eggs of A. intermedia. Considering these data, under specific conditions, hybrids can be expected to be produced and survive in nature in the Pacific. Furthermore, we identified one colony with intermediate morphology between A. florida and A. intermedia in the field. This colony was fertilized only by eggs of A. florida, with high fertilization rates, suggesting that this colony would be a hybrid of these two species and might be backcrossed.