A real-time PCR assay for the detection of atypical strains of Chlamydiaceae from pigeons

Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows spec...

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Bibliographic Details
Published in:PloS one 2013-03, Vol.8 (3), p.e58741-e58741
Main Authors: Zocevic, Aleksandar, Vorimore, Fabien, Vicari, Nadia, Gasparini, Julien, Jacquin, Lisa, Sachse, Konrad, Magnino, Simone, Laroucau, Karine
Format: Article
Language:English
Subjects:
Animals
Assaying
Biology
Birds
Chlamydia
Chlamydia psittaci
Chlamydiaceae - classification
Chlamydiaceae - enzymology
Chlamydiaceae - genetics
Chlamydiaceae - isolation & purification
Chlamydiae
Columbidae - microbiology
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Epidemiology
Genetic testing
Genome, Bacterial - genetics
Laboratories
Medicine
Parrot fever
Phosphopyruvate Hydratase - genetics
Phylogeny
Pigeons
Poultry
Real time
Real-Time Polymerase Chain Reaction - methods
Reproducibility of Results
Salmonella
Salmonella Enteritidis
Veterinary Science
Zoonoses
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Summary:Recent evidence of the occurrence of atypical Chlamydiaceae strains in pigeons, different from the established Chlamydiaceae, requires the development of a specific and rapid detection tool to investigate their prevalence and significance. Here is described a new real-time PCR assay that allows specific detection of atypical Chlamydiaceae from pigeons. The assay has been used to assess the dissemination of these strains in field samples collected from Parisian pigeon populations in 2009. The results suggest a limited dissemination compared to the usually higher prevalence of Chlamydia psittaci that is the main species associated with avian chlamydiosis.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0058741