Dendritic cell podosome dynamics does not depend on the F-actin regulator SWAP-70

• Published in: PloS one 2013-03, Vol.8 (3), p.e60642
• Main Authors: Götz, Anne, Jessberger, Rolf
• Format: Article
• Language: English
• Subjects: Actin; Actins - metabolism; Adhesion; Animals; Antigens; Biocompatibility; Biology; Biomedical materials; Bone growth; Bone matrix; Bone resorption; Cell adhesion & migration; Cell Membrane Structures - metabolism; Cellular structure; Connective tissues; Degradation; Dendritic cells; Dendritic Cells - cytology; Dendritic Cells - metabolism; Dendritic structure; Diapedesis; Dismantling; DNA-Binding Proteins - deficiency; DNA-Binding Proteins - metabolism; Extracellular matrix; Extracellular Space - metabolism; Filopodia; Gelatin - metabolism; Guanine Nucleotide Exchange Factors - deficiency; Guanine Nucleotide Exchange Factors - metabolism; Humans; Immune system; Leukocyte migration; Lipopolysaccharides - pharmacology; Lymphatic system; Lymphocytes; Macrophages; Matrix metalloproteinases; Mice; Minor Histocompatibility Antigens; Nuclear Proteins - deficiency; Nuclear Proteins - metabolism; Osteoclasts; Physiology; Protein Transport; Proteins; Recovery (Medical); T cell receptors; TLR4 protein; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Toll-Like Receptors - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0060642

In addition to classical adhesion structures like filopodia or focal adhesions, dendritic cells similar to macrophages and osteoclasts assemble highly dynamic F-actin structures called podosomes. They are involved in cellular processes such as extracellular matrix degradation, bone resorption by osteoclasts, and trans-cellular diapedesis of lymphocytes. Besides adhesion and migration, podosomes enable dendritic cells to degrade connective tissue by matrix metalloproteinases. SWAP-70 interacts with RhoGTPases and F-actin and regulates migration of dendritic cells. SWAP-70 deficient osteoclasts are impaired in F-actin-ring formation and bone resorption. In the present study, we demonstrate that SWAP-70 is not required for podosome formation and F-actin turnover in dendritic cells. Furthermore, we found that toll-like receptor 4 ligand induced podosome disassembly and podosome-mediated matrix degradation is not affected by SWAP-70 in dendritic cells. Thus, podosome formation and function in dendritic cells is independent of SWAP-70.