Insulin-receptor substrate-2 (irs-2) is required for maintaining glucokinase and glucokinase regulatory protein expression in mouse liver

Insulin receptor substrate (IRS) proteins play important roles in hepatic nutrient homeostasis. Since glucokinase (GK) and glucokinase regulatory protein (GKRP) function as key glucose sensors, we have investigated the expression of GK and GKRP in liver of Irs-2 deficient mice and Irs2(-/-) mice whe...

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Bibliographic Details
Published in:PloS one 2013-04, Vol.8 (4), p.e58797-e58797
Main Authors: Roncero, Isabel, Alvarez, Elvira, Acosta, Carlos, Sanz, Carmen, Barrio, Pedro, Hurtado-Carneiro, Veronica, Burks, Deborah, Blázquez, Enrique
Format: Article
Language:English
Subjects:
Animal models
Animals
Biology
Brain
Carrier Proteins - genetics
Carrier Proteins - metabolism
Gene expression
Gene Expression Regulation
Genetic Complementation Test
Glucokinase
Glucokinase - genetics
Glucokinase - metabolism
Glucose
Glucose - metabolism
Health aspects
Homeostasis
Hypothalamus
Hypothalamus - metabolism
Insulin
Insulin - metabolism
Insulin Receptor Substrate Proteins - deficiency
Insulin Receptor Substrate Proteins - genetics
Insulin resistance
Insulin-Secreting Cells - cytology
Insulin-Secreting Cells - metabolism
Kinases
Liver
Liver - metabolism
Medicine
Mice
Mice, Knockout
mRNA
Organ Specificity
Pancreas
Pancreatic beta cells
Post-translation
Proteins
Receptors
RNA, Messenger - genetics
Rodents
Signal Transduction
Signaling
Substrates
Transfection
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Summary:Insulin receptor substrate (IRS) proteins play important roles in hepatic nutrient homeostasis. Since glucokinase (GK) and glucokinase regulatory protein (GKRP) function as key glucose sensors, we have investigated the expression of GK and GKRP in liver of Irs-2 deficient mice and Irs2(-/-) mice where Irs2 was reintroduced specifically into pancreatic β-cells [RIP-Irs-2/IRS-2(-/-)]. We observed that liver GK activity was significantly lower (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0058797