Age-dependent effects of oral infection with dengue virus on Aedes aegypti (Diptera: Culicidae) feeding behavior, survival, oviposition success and fecundity

• Published in: PloS one 2013-03, Vol.8 (3), p.e59933-e59933
• Main Authors: Sylvestre, Gabriel, Gandini, Mariana, Maciel-de-Freitas, Rafael
• Format: Article
• Language: English
• Subjects: Aedes - virology; Aedes aegypti; Aedes albopictus; Age; Age Factors; Animals; Aquatic insects; Behavior, Animal; Biology; Blood; Culicidae; Dengue; Dengue - transmission; Dengue fever; Dengue Virus; Eggs; Erythrocytes - virology; Fecundity; Feeding; Feeding Behavior; Female; Females; Fertility; Infections; Insect Vectors; Laboratories; Life span; Medicine; Mice; Mortality; Mosquitoes; Oral infection; Oviposition; Physiology; Population density; Public health; Rabbits; Survival; Vector-borne diseases; Viral diseases; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0059933

Aedes aegypti is the main vector of dengue, a disease that is increasing its geographical range as well as incidence rates. Despite its public health importance, the effect of dengue virus (DENV) on some mosquito traits remains unknown. Here, we investigated the impact of DENV-2 infection on the feeding behavior, survival, oviposition success and fecundity of Ae. aegypti females. After orally-challenging Ae. aegypti females with a DENV-2 strain using a membrane feeder, we monitored the feeding behavior, survival, oviposition success and fecundity throughout the mosquito lifespan. We observed an age-dependent cost of DENV infection on mosquito feeding behavior and fecundity. Infected individuals took more time to ingest blood from anesthetized mice in the 2(nd) and 3(rd) weeks post-infection, and also longer overall blood-feeding times in the 3(rd) week post-infection, when females were around 20 days old. Often, infected Ae. aegypti females did not lay eggs and when they were laid, smaller number of eggs were laid compared to uninfected controls. A reduction in the number of eggs laid per female was evident starting on the 3(rd) week post-infection. DENV-2 negatively affected mosquito lifespan, since overall the longevity of infected females was halved compared to that of the uninfected control group. The DENV-2 strain tested significantly affected Ae. aegypti traits directly correlated with vectorial capacity or mosquito population density, such as feeding behavior, survival, fecundity and oviposition success. Infected mosquitoes spent more time ingesting blood, had reduced lifespan, laid eggs less frequently, and when they did lay eggs, the clutches were smaller than uninfected mosquitoes.