Caveolar fatty acids and acylation of caveolin-1

Caveolae are cholesterol and sphingolipids rich subcellular domains on plasma membrane. Caveolae contain a variety of signaling proteins which provide platforms for signaling transduction. In addition to enriched with cholesterol and sphingolipids, caveolae also contain a variety of fatty acids. It...

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Published in:PloS one 2013-04, Vol.8 (4), p.e60884-e60884
Main Authors: Cai, Qian, Guo, Ling, Gao, Haiqing, Li, Xiang-An
Format: Article
Language:English
Subjects:
Acylation
Analysis
Animals
Biology
Blotting, Western
Caveolae
Caveolae - chemistry
Caveolin
Caveolin 1 - metabolism
Caveolin-1
Cellular signal transduction
Chemistry
CHO Cells
Cholesterol
Chromatography
Cricetinae
Cricetulus
Esterification
Fatty acids
Fatty Acids - analysis
Gas chromatography
Gas Chromatography-Mass Spectrometry
Geriatrics
Immunoglobulin G
Immunoprecipitation
Kinases
Laboratories
Lipids
Mass spectrometry
Nitric oxide
Oleic acid
Palmitic acid
Pediatrics
Penicillin
Proteins
Rodents
Saturated fatty acids
Signal transduction
Signaling
Sphingolipids
Stearic acid
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cited_by cdi_FETCH-LOGICAL-c758t-12495cf15c279c85b05e7b5efb4266c1c741aa3ee53c31d270ced25eccd3bcfd3
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Guo, Ling
Gao, Haiqing
Li, Xiang-An
description Caveolae are cholesterol and sphingolipids rich subcellular domains on plasma membrane. Caveolae contain a variety of signaling proteins which provide platforms for signaling transduction. In addition to enriched with cholesterol and sphingolipids, caveolae also contain a variety of fatty acids. It has been well-established that acylation of protein plays a pivotal role in subcellular location including targeting to caveolae. However, the fatty acid compositions of caveolae and the type of acylation of caveolar proteins remain largely unknown. In this study, we investigated the fatty acids in caveolae and caveolin-1 bound fatty acids. Caveolae were isolated from Chinese hamster ovary (CHO) cells. The caveolar fatty acids were extracted with Folch reagent, methyl esterificated with BF3, and analyzed by gas chromatograph-mass spectrometer (GC/MS). The caveolin-1 bound fatty acids were immunoprecipitated by anti-caveolin-1 IgG and analyzed with GC/MS. In contrast to the whole CHO cell lysate which contained a variety of fatty acids, caveolae mainly contained three types of fatty acids, 0.48 µg palmitic acid, 0.61 µg stearic acid and 0.83 µg oleic acid/caveolae preparation/5 × 10(7) cells. Unexpectedly, GC/MS analysis indicated that caveolin-1 was not acylated by myristic acid; instead, it was acylated by palmitic acid and stearic acid. Caveolae contained a special set of fatty acids, highly enriched with saturated fatty acids, and caveolin-1 was acylated by palmitic acid and stearic acid. The unique fatty acid compositions of caveolae and acylation of caveolin-1 may be important for caveolae formation and for maintaining the function of caveolae.
doi_str_mv 10.1371/journal.pone.0060884
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In contrast to the whole CHO cell lysate which contained a variety of fatty acids, caveolae mainly contained three types of fatty acids, 0.48 µg palmitic acid, 0.61 µg stearic acid and 0.83 µg oleic acid/caveolae preparation/5 × 10(7) cells. Unexpectedly, GC/MS analysis indicated that caveolin-1 was not acylated by myristic acid; instead, it was acylated by palmitic acid and stearic acid. Caveolae contained a special set of fatty acids, highly enriched with saturated fatty acids, and caveolin-1 was acylated by palmitic acid and stearic acid. The unique fatty acid compositions of caveolae and acylation of caveolin-1 may be important for caveolae formation and for maintaining the function of caveolae.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23593340</pmid><doi>10.1371/journal.pone.0060884</doi><tpages>e60884</tpages><oa>free_for_read</oa></addata></record>
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1932-6203
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subjects Acylation
Analysis
Animals
Biology
Blotting, Western
Caveolae
Caveolae - chemistry
Caveolin
Caveolin 1 - metabolism
Caveolin-1
Cellular signal transduction
Chemistry
CHO Cells
Cholesterol
Chromatography
Cricetinae
Cricetulus
Esterification
Fatty acids
Fatty Acids - analysis
Gas chromatography
Gas Chromatography-Mass Spectrometry
Geriatrics
Immunoglobulin G
Immunoprecipitation
Kinases
Laboratories
Lipids
Mass spectrometry
Nitric oxide
Oleic acid
Palmitic acid
Pediatrics
Penicillin
Proteins
Rodents
Saturated fatty acids
Signal transduction
Signaling
Sphingolipids
Stearic acid
title Caveolar fatty acids and acylation of caveolin-1
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