Effect of dietary advanced glycation end products on mouse liver

• Published in: PloS one 2012-04, Vol.7 (4), p.e35143
• Main Authors: Patel, Raza, Baker, Susan S, Liu, Wensheng, Desai, Sonal, Alkhouri, Razan, Kozielski, Rafal, Mastrandrea, Lucy, Sarfraz, Adil, Cai, Weijing, Vlassara, Helen, Patel, Mulchand S, Baker, Robert D, Zhu, Lixin
• Format: Article
• Language: English
• Subjects: Advanced glycosylation end products; Age; Alanine; Alanine transaminase; Alanine Transaminase - analysis; Alzheimer's disease; Animals; Aspartate; Aspartate aminotransferase; Aspartate Aminotransferases - analysis; Atherosclerosis; Biology; Diabetes; Diet; Diet - adverse effects; Enzymes; Fasting; Fasting - blood; Fatty liver; Fatty Liver - chemically induced; Fatty Liver - enzymology; Fatty Liver - pathology; Food; Glucose; Glucose tolerance; Glucose Tolerance Test; Glycation End Products, Advanced - administration & dosage; Glycosylation; Histology; Hospitals; Hypotheses; Infiltration; Inflammation; Insulin; Insulin - blood; Insulin Resistance; Laboratories; Liver; Liver - cytology; Liver - drug effects; Liver - enzymology; Liver diseases; Male; Medicine; Melanoma; Metastasis; Mice; Mice, Inbred C57BL; Nephrology; Neutrophil Infiltration - drug effects; Nutrition research; Oxidative stress; Pathogenesis; Pediatrics; Proteins; Steatosis; Womens health
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0035143

The exact pathophysiology of non-alcoholic steatohepatitis (NASH) is not known. Previous studies suggest that dietary advanced glycation end products (AGEs) can cause oxidative stress in liver. We aim to study the effects of dietary AGEs on liver health and their possible role in the pathogenesis of NASH. Two groups of mice were fed the same diet except the AGE content varied. One group was fed a high AGE diet and the second group was fed a regular AGE diet. Liver histology, alanine aminotransferase, aspartate aminotransferase, fasting glucose, fasting insulin, insulin resistance and glucose tolerance were assessed. Histology revealed that neutrophil infiltration occurred in the livers of the high AGE group at week 26; steatosis did not accompany liver inflammation. At week 39 livers from both groups exhibited macro- or micro-steatosis, yet no inflammation was detected. Higher insulin levels were detected in the regular AGE group at week 26 (P = 0.034), compared to the high AGE group. At week 39, the regular AGE group showed higher levels of alanine aminotransferase (P