The isolation and characterization of β-glucogallin as a novel aldose reductase inhibitor from Emblica officinalis

• Published in: PloS one 2012-04, Vol.7 (4), p.e31399-e31399
• Main Authors: Puppala, Muthenna, Ponder, Jessica, Suryanarayana, Palla, Reddy, Geereddy Bhanuprakash, Petrash, J Mark, LaBarbera, Daniel V
• Format: Article
• Language: English
• Subjects: Aldehyde reductase; Aldehyde Reductase - antagonists & inhibitors; Aldehyde Reductase - chemistry; Animals; Bioassays; Biochemistry; Biology; Blindness; Catalysis; Catalytic Domain; Cataracts; Chemistry; Chromatography; Complications; Computer Simulation; Diabetes; Diabetes mellitus; Diabetic retinopathy; Emblica officinalis; Enzymes; Eye diseases; Eye lens; Fruit - chemistry; Glucose; Humans; Hydrobromic Acid; Hydrolyzable Tannins - chemistry; Hydrolyzable Tannins - isolation & purification; Hydrolyzable Tannins - pharmacology; Inhibitors; Lens, Crystalline - drug effects; Lens, Crystalline - enzymology; Lens, Crystalline - metabolism; Ligands; Medical treatment; Medicine; Mice; Models, Molecular; Molecular modelling; Mutagenesis; Natural products; Nutrition; Organ culture; Oxidation-Reduction; Pathogenesis; Pharmaceutical sciences; Pharmacy; Phyllanthus emblica - chemistry; Plant extracts; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Protein Binding; Retinopathy; Sorbitol; Sorbitol - metabolism; Tissue Culture Techniques; Transgenic mice
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0031399

Diabetes mellitus is recognized as a leading cause of new cases of blindness. The prevalence of diabetic eye disease is expected to continue to increase worldwide as a result of the dramatic increase in the number of people with diabetes. At present, there is no medical treatment to delay or prevent the onset and progression of cataract or retinopathy, the most common causes of vision loss in diabetics. The plant Emblica officinalis (gooseberry) has been used for thousands of years as a traditional Indian Ayurvedic preparation for the treatment of diabetes in humans. Extracts from this plant have been shown to be efficacious against the progression of cataract in a diabetic rat model. Aldose reductase (ALR2) is implicated in the development of secondary complications of diabetes including cataract and, therefore, has been a major drug target for the development of therapies to treat diabetic disease. Herein, we present the bioassay-guided isolation and structure elucidation of 1-O-galloyl-β-D-glucose (β-glucogallin), a major component from the fruit of the gooseberry that displays selective as well as relatively potent inhibition (IC(50) = 17 µM) of AKR1B1 in vitro. Molecular modeling demonstrates that this inhibitor is able to favorably bind in the active site. Further, we show that β-glucogallin effectively inhibits sorbitol accumulation by 73% at 30 µM under hyperglycemic conditions in an ex-vivo organ culture model of lenses excised from transgenic mice overexpressing human ALR2 in the lens. This study supports the continued development of natural products such as β-glucogallin as therapeutic leads in the development of novel therapies to treat diabetic complications such as cataract.