Anti-hyperlipidemic effects and potential mechanisms of action of the caffeoylquinic acid-rich Pandanus tectorius fruit extract in hamsters fed a high fat-diet

• Published in: PloS one 2013-04, Vol.8 (4), p.e61922
• Main Authors: Zhang, Xiaopo, Wu, Chongming, Wu, Haifeng, Sheng, Linghui, Su, Yan, Zhang, Xue, Luan, Hong, Sun, Guibo, Sun, Xiaobo, Tian, Yu, Ji, Yubin, Guo, Peng, Xu, Xudong
• Format: Article
• Language: English
• Subjects: AMP; AMP-activated protein kinase; Animals; Anticholesteremic agents; Butanol; Caffeoylquinic acid; Cardiovascular disease; Cardiovascular diseases; Chemistry; Cholesterol; Cholesterol - blood; Cholesterol - metabolism; Cholesterol, LDL - blood; Cholesterol, LDL - metabolism; Cricetinae; Diabetes; Diet; Diet, High-Fat - adverse effects; Enzymes; Ethanol; Fatty acids; Folk medicine; Fruit - chemistry; Fruits; Gene expression; Genes; Hamsters; Heart diseases; Herbal medicine; Hyperlipidemia; Hypolipidemic Agents - therapeutic use; Kinases; Life sciences; Lipase; Lipid Metabolism - drug effects; Lipids; Lipoprotein (low density) receptors; Lipoprotein lipase; Lipoproteins; Lipoproteins (low density); Liver; Liver - drug effects; Liver - metabolism; Low density lipoprotein; Low density lipoprotein receptors; Low density lipoproteins; Magnetic resonance; Medicine; Metabolism; Metabolites; NMR; Nuclear magnetic resonance; Obesity; Pandanaceae - chemistry; Pandanus tectorius; Pharmacology; Phosphorylation; Plant Extracts - therapeutic use; Proteins; Quinic Acid - analogs & derivatives; Quinic Acid - therapeutic use; Risk analysis; Risk factors; Rodents; Serum levels; Spectrum analysis; Toxicology; Transcription; Triglycerides; Triglycerides - blood; Triglycerides - metabolism
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0061922

Hyperlipidemia is considered to be one of the greatest risk factors contributing to the prevalence and severity of cardiovascular diseases. In this work, we investigated the anti-hyperlipidemic effect and potential mechanism of action of the Pandanus tectorius fruit extract in hamsters fed a high fat-diet (HFD). The n-butanol fraction of the P. tectorius fruit ethanol extract (PTF-b) was rich in caffeoylquinic acids (CQAs). Administration of PTF-b for 4 weeks effectively decreased retroperitoneal fat and the serum levels of total cholesterol (TC), triglycerides (TG) and low density lipoprotein-cholesterol (LDL-c) and hepatic TC and TG. The lipid signals (fatty acids, and cholesterol) in the liver as determined by nuclear magnetic resonance (NMR) were correspondingly reduced. Realtime quantitative PCR showed that the mRNA levels of PPARα and PPARα-regulated genes such as ACO, CPT1, LPL and HSL were largely enhanced by PTF-b. The transcription of LDLR, CYP7A1, and PPARγ was also upregulated. Treatment with PTF-b significantly stimulated the activation of AMP-activated protein kinase (AMPK) as well as the activity of serum and hepatic lipoprotein lipase (LPL). Together, these results suggest that administration of the PTF-b enriched in CQAs moderates hyperlipidemia and improves the liver lipid profile. These effects may be caused, at least in part, by increasing the expression of PPARα and its downstream genes and by upregulation of LPL and AMPK activities.