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Quercetin protects Saccharomyces cerevisiae against oxidative stress by inducing trehalose biosynthesis and the cell wall integrity pathway
Quercetin is a naturally occurring flavonol with antioxidant, anticancer and anti-ageing properties. In this study we aimed to identify genes differentially expressed in yeast cells treated with quercetin and its role in oxidative stress protection. A microarray analysis was performed to characteriz...
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| Published in: | PloS one 2012-09, Vol.7 (9), p.e45494 |
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| Main Authors: | , , , , , , , , |
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| container_issue | 9 |
| container_start_page | e45494 |
| container_title | PloS one |
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| creator | Vilaça, Rita Mendes, Vanda Mendes, Marta Vaz Carreto, Laura Amorim, Maria Amélia de Freitas, Victor Moradas-Ferreira, Pedro Mateus, Nuno Costa, Vítor |
| description | Quercetin is a naturally occurring flavonol with antioxidant, anticancer and anti-ageing properties. In this study we aimed to identify genes differentially expressed in yeast cells treated with quercetin and its role in oxidative stress protection.
A microarray analysis was performed to characterize changes in the transcriptome and the expression of selected genes was validated by RT-qPCR. Biological processes significantly affected were identified by using the FUNSPEC software and their relevance in H(2)O(2) resistance induced by quercetin was assessed.
Genes associated with RNA metabolism and ribosome biogenesis were down regulated in cells treated with quercetin, whereas genes associated with carbohydrate metabolism, endocytosis and vacuolar proteolysis were up regulated. The induction of genes related to the metabolism of energy reserves, leading to the accumulation of the stress protectant disaccharide trehalose, and the activation of the cell wall integrity pathway play a key role in oxidative stress resistance induced by quercetin.
These results suggest that quercetin may act as a modulator of cell signaling pathways related to carbohydrate metabolism and cell integrity to exert its protective effects against oxidative stress. |
| doi_str_mv | 10.1371/journal.pone.0045494 |
| format | article |
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A microarray analysis was performed to characterize changes in the transcriptome and the expression of selected genes was validated by RT-qPCR. Biological processes significantly affected were identified by using the FUNSPEC software and their relevance in H(2)O(2) resistance induced by quercetin was assessed.
Genes associated with RNA metabolism and ribosome biogenesis were down regulated in cells treated with quercetin, whereas genes associated with carbohydrate metabolism, endocytosis and vacuolar proteolysis were up regulated. The induction of genes related to the metabolism of energy reserves, leading to the accumulation of the stress protectant disaccharide trehalose, and the activation of the cell wall integrity pathway play a key role in oxidative stress resistance induced by quercetin.
These results suggest that quercetin may act as a modulator of cell signaling pathways related to carbohydrate metabolism and cell integrity to exert its protective effects against oxidative stress.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0045494</identifier><identifier>PMID: 23029052</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actins - metabolism ; Analysis ; Anticancer properties ; Antioxidants ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Apoptosis ; Baking yeast ; Biological activity ; Biology ; Biosynthesis ; Carbohydrate metabolism ; Carbohydrates ; Cell activation ; Cell cycle ; Cell Wall - metabolism ; Cell walls ; Cellular signal transduction ; Chemistry ; Colorectal cancer ; DNA microarrays ; Endocytosis ; Energy metabolism ; Energy reserves ; Flavonoids ; Gene expression ; Gene Expression Profiling ; Gene Expression Regulation, Fungal - drug effects ; Genes ; Genetic aspects ; Glycogen - metabolism ; Glycolysis - drug effects ; Homeostasis ; Hydrogen peroxide ; Hydrogen Peroxide - pharmacology ; Integrity ; Kinases ; Metabolism ; Metabolites ; Nitrogen dioxide ; Oxidation resistance ; Oxidative Stress ; Physiological aspects ; Polyphenols ; Proteins ; Proteolysis ; Quercetin ; Quercetin - chemistry ; Quercetin - pharmacology ; Ribonucleic acid ; RNA ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - drug effects ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - metabolism ; Signal transduction ; Signal Transduction - drug effects ; Signaling ; Transcription factors ; Trehalose ; Trehalose - biosynthesis ; Yeast</subject><ispartof>PloS one, 2012-09, Vol.7 (9), p.e45494</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Vilaça et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Vilaça et al 2012 Vilaça et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c785t-81c769a1c76aa07ddfba1e90ed6dded942e6906ea55d5efa4b1251a49be1b9163</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1344508722/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1344508722?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23029052$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Kowaltowski, Alicia J.</contributor><creatorcontrib>Vilaça, Rita</creatorcontrib><creatorcontrib>Mendes, Vanda</creatorcontrib><creatorcontrib>Mendes, Marta Vaz</creatorcontrib><creatorcontrib>Carreto, Laura</creatorcontrib><creatorcontrib>Amorim, Maria Amélia</creatorcontrib><creatorcontrib>de Freitas, Victor</creatorcontrib><creatorcontrib>Moradas-Ferreira, Pedro</creatorcontrib><creatorcontrib>Mateus, Nuno</creatorcontrib><creatorcontrib>Costa, Vítor</creatorcontrib><title>Quercetin protects Saccharomyces cerevisiae against oxidative stress by inducing trehalose biosynthesis and the cell wall integrity pathway</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Quercetin is a naturally occurring flavonol with antioxidant, anticancer and anti-ageing properties. In this study we aimed to identify genes differentially expressed in yeast cells treated with quercetin and its role in oxidative stress protection.
A microarray analysis was performed to characterize changes in the transcriptome and the expression of selected genes was validated by RT-qPCR. Biological processes significantly affected were identified by using the FUNSPEC software and their relevance in H(2)O(2) resistance induced by quercetin was assessed.
Genes associated with RNA metabolism and ribosome biogenesis were down regulated in cells treated with quercetin, whereas genes associated with carbohydrate metabolism, endocytosis and vacuolar proteolysis were up regulated. The induction of genes related to the metabolism of energy reserves, leading to the accumulation of the stress protectant disaccharide trehalose, and the activation of the cell wall integrity pathway play a key role in oxidative stress resistance induced by quercetin.
These results suggest that quercetin may act as a modulator of cell signaling pathways related to carbohydrate metabolism and cell integrity to exert its protective effects against oxidative stress.</description><subject>Actins - metabolism</subject><subject>Analysis</subject><subject>Anticancer properties</subject><subject>Antioxidants</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Baking yeast</subject><subject>Biological activity</subject><subject>Biology</subject><subject>Biosynthesis</subject><subject>Carbohydrate metabolism</subject><subject>Carbohydrates</subject><subject>Cell activation</subject><subject>Cell cycle</subject><subject>Cell Wall - metabolism</subject><subject>Cell walls</subject><subject>Cellular signal transduction</subject><subject>Chemistry</subject><subject>Colorectal cancer</subject><subject>DNA microarrays</subject><subject>Endocytosis</subject><subject>Energy metabolism</subject><subject>Energy reserves</subject><subject>Flavonoids</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal - 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drug effects</subject><subject>Signaling</subject><subject>Transcription factors</subject><subject>Trehalose</subject><subject>Trehalose - biosynthesis</subject><subject>Yeast</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk21rFDEQxxdRbK1-A9GAIPjiziSbfcgboRQfCoWiVd-G2WRuN2UvuSbZtvcZ_NLmvGvpgYIEkmHym3_-DJmieMnonJUNe3_pp-BgnK-8wzmlohJSPCoOmSz5rOa0fPwgPiiexXhJaVW2df20OOAl5ZJW_LD49XXCoDFZR1bBJ9QpkgvQeoDgl2uNkWgMeG2jBSTQg3UxEX9rDSR7jSSmgDGSbk2sM5O2ric5M8DoI5LO-rh2acBoIwFnSA6z3DiSG8ibdQn7YNOarCANN7B-XjxZwBjxxe48Kn58-vj95Mvs7Pzz6cnx2Uw3bZVmLdNNLWGzA9DGmEUHDCVFUxuDRgqOtaQ1QlWZChcgOsYrBkJ2yDrJ6vKoeL3VXWWbatfHqFgpREXbhvNMnG4J4-FSrYJdQlgrD1b9SfjQKwjJ6hFVWWPbsFaCaISQjZSaM2RtrVvNayrKrPVh99rULdFodCnAuCe6f-PsoHp_rTZ2qnJj5s1OIPirCWP6h-Ud1UN2Zd3CZzG9tFGrYyFbXnPBm0zN_0LlZXBpdf5JC5vzewXv9goyk_A29TDFqE4vvv0_e_5zn337gB0QxjREP07Jehf3QbEFdfAxBlzcd45RtRmEu26ozSCo3SDkslcPu35fdPfzy9-WAgb2</recordid><startdate>20120918</startdate><enddate>20120918</enddate><creator>Vilaça, Rita</creator><creator>Mendes, Vanda</creator><creator>Mendes, Marta Vaz</creator><creator>Carreto, Laura</creator><creator>Amorim, Maria Amélia</creator><creator>de Freitas, Victor</creator><creator>Moradas-Ferreira, Pedro</creator><creator>Mateus, Nuno</creator><creator>Costa, Vítor</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120918</creationdate><title>Quercetin protects Saccharomyces cerevisiae against oxidative stress by inducing trehalose biosynthesis and the cell wall integrity pathway</title><author>Vilaça, Rita ; Mendes, Vanda ; Mendes, Marta Vaz ; Carreto, Laura ; Amorim, Maria Amélia ; de Freitas, Victor ; Moradas-Ferreira, Pedro ; Mateus, Nuno ; Costa, Vítor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c785t-81c769a1c76aa07ddfba1e90ed6dded942e6906ea55d5efa4b1251a49be1b9163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Actins - 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In this study we aimed to identify genes differentially expressed in yeast cells treated with quercetin and its role in oxidative stress protection.
A microarray analysis was performed to characterize changes in the transcriptome and the expression of selected genes was validated by RT-qPCR. Biological processes significantly affected were identified by using the FUNSPEC software and their relevance in H(2)O(2) resistance induced by quercetin was assessed.
Genes associated with RNA metabolism and ribosome biogenesis were down regulated in cells treated with quercetin, whereas genes associated with carbohydrate metabolism, endocytosis and vacuolar proteolysis were up regulated. The induction of genes related to the metabolism of energy reserves, leading to the accumulation of the stress protectant disaccharide trehalose, and the activation of the cell wall integrity pathway play a key role in oxidative stress resistance induced by quercetin.
These results suggest that quercetin may act as a modulator of cell signaling pathways related to carbohydrate metabolism and cell integrity to exert its protective effects against oxidative stress.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23029052</pmid><doi>10.1371/journal.pone.0045494</doi><tpages>e45494</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-09, Vol.7 (9), p.e45494 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1344508722 |
| source | Publicly Available Content Database (Proquest) (PQ_SDU_P3); PubMed Central |
| subjects | Actins - metabolism Analysis Anticancer properties Antioxidants Antioxidants - chemistry Antioxidants - pharmacology Apoptosis Baking yeast Biological activity Biology Biosynthesis Carbohydrate metabolism Carbohydrates Cell activation Cell cycle Cell Wall - metabolism Cell walls Cellular signal transduction Chemistry Colorectal cancer DNA microarrays Endocytosis Energy metabolism Energy reserves Flavonoids Gene expression Gene Expression Profiling Gene Expression Regulation, Fungal - drug effects Genes Genetic aspects Glycogen - metabolism Glycolysis - drug effects Homeostasis Hydrogen peroxide Hydrogen Peroxide - pharmacology Integrity Kinases Metabolism Metabolites Nitrogen dioxide Oxidation resistance Oxidative Stress Physiological aspects Polyphenols Proteins Proteolysis Quercetin Quercetin - chemistry Quercetin - pharmacology Ribonucleic acid RNA Saccharomyces cerevisiae Saccharomyces cerevisiae - drug effects Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism Signal transduction Signal Transduction - drug effects Signaling Transcription factors Trehalose Trehalose - biosynthesis Yeast |
| title | Quercetin protects Saccharomyces cerevisiae against oxidative stress by inducing trehalose biosynthesis and the cell wall integrity pathway |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T13%3A30%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quercetin%20protects%20Saccharomyces%20cerevisiae%20against%20oxidative%20stress%20by%20inducing%20trehalose%20biosynthesis%20and%20the%20cell%20wall%20integrity%20pathway&rft.jtitle=PloS%20one&rft.au=Vila%C3%A7a,%20Rita&rft.date=2012-09-18&rft.volume=7&rft.issue=9&rft.spage=e45494&rft.pages=e45494-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0045494&rft_dat=%3Cgale_plos_%3EA498262427%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c785t-81c769a1c76aa07ddfba1e90ed6dded942e6906ea55d5efa4b1251a49be1b9163%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1344508722&rft_id=info:pmid/23029052&rft_galeid=A498262427&rfr_iscdi=true |