Renalase's expression and distribution in renal tissue and cells

To study renalase's expression and distribution in renal tissues and cells, renalase coded DNA vaccine was constructed, and anti-renalase monoclonal antibodies were produced using DNA immunization and hybridoma technique, followed by further investigation with immunological testing and western...

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Bibliographic Details
Published in:PloS one 2012-10, Vol.7 (10), p.e46442-e46442
Main Authors: Wang, Feng, Xing, Tao, Li, Junhui, Bai, Mei, Hu, Ruimin, Zhao, Zhonghua, Tian, Shoufu, Zhang, Zhigang, Wang, Niansong
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal - immunology
Base Sequence
Binding sites
Biology
Blotting, Western
Deoxyribonucleic acid
Disease
DNA
DNA Primers
DNA vaccines
Enzymes
Epithelial cells
Fluorescent Antibody Technique
Gene expression
Genetic research
Heart
Hospitals
Hypertension
Immunization
Immunoglobulins
Immunohistochemistry
Immunology
Kidney - cytology
Kidney - enzymology
Kidneys
Medicine
Mesangial cells
Mice
Mice, Inbred BALB C
Monoamine Oxidase - immunology
Monoamine Oxidase - metabolism
Monoclonal antibodies
Nephrology
Pathology
Plasmids
Proteins
Rheumatology
Rodents
Tissues
Type 2 diabetes
Vaccines
Western blotting
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Summary:To study renalase's expression and distribution in renal tissues and cells, renalase coded DNA vaccine was constructed, and anti-renalase monoclonal antibodies were produced using DNA immunization and hybridoma technique, followed by further investigation with immunological testing and western blotting to detect the expression and distribution of renalase among the renal tissue and cells. Anti-renalase monoclonal antibodies were successfully prepared by using DNA immunization technique. Further studies with anti-renalase monoclonal antibody showed that renalase expressed in glomeruli, tubule, mesangial cells, podocytes, renal tubule epithelial cells and its cells supernatant. Renalase is wildly expressed in kidney, including glomeruli, tubule, mesangial cells, podocytes and tubule epithelial cells, and may be secreted by tubule epithelial cells primarily.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0046442