The motor protein KIF14 inhibits tumor growth and cancer metastasis in lung adenocarcinoma

• Published in: PloS one 2013-04, Vol.8 (4), p.e61664-e61664
• Main Authors: Hung, Pei-Fang, Hong, Tse-Ming, Hsu, Yi-Chiung, Chen, Hsuan-Yu, Chang, Yih-Leong, Wu, Chen-Tu, Chang, Gee-Chen, Jou, Yuh-Shan, Pan, Szu-Hua, Yang, Pan-Chyr
• Format: Article
• Language: English
• Subjects: Adenocarcinoma; Adenocarcinoma - genetics; Adenocarcinoma - metabolism; Adenocarcinoma - mortality; Adenocarcinoma - pathology; Adenocarcinoma of Lung; Adhesion; Aged; Anaphase; Animals; Biology; Brain cancer; Bronchi - cytology; Bronchi - metabolism; Cadherins; Cadherins - genetics; Cadherins - metabolism; Cancer; Cancer metastasis; Cancer research; Cancer treatment; CD146 Antigen - genetics; CD146 Antigen - metabolism; Cell Adhesion; Cell adhesion molecules; Cell cycle; Cell migration; Cell Movement; Chromosome 1; Chromosomes; Collagen (type IV); Collagen Type IV - metabolism; Comparative analysis; Comparative Genomic Hybridization; Cytogenetics; Development and progression; Epithelial cells; Epithelial Cells - cytology; Epithelial Cells - metabolism; Extracellular matrix; Female; Gene expression; Gene Expression Regulation, Neoplastic; Genomics; Growth; Heterozygosity; Humans; Hybridization; Internal medicine; Invasiveness; Kinases; Kinesin; Kinesin - antagonists & inhibitors; Kinesin - genetics; Kinesin - metabolism; Laminin; Laminin - metabolism; Liver cancer; Loss of heterozygosity; Lung cancer; Lung Neoplasms - genetics; Lung Neoplasms - metabolism; Lung Neoplasms - mortality; Lung Neoplasms - pathology; Male; Medicine; Melanoma; Metaphase; Metastases; Metastasis; Mice; Mice, SCID; Middle Aged; mRNA; Neoplasm Metastasis; Oncogene Proteins - antagonists & inhibitors; Oncogene Proteins - genetics; Oncogene Proteins - metabolism; Pathology; Prognosis; Protein Binding; Protein expression; Proteins; Proteomics; RNA; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Survival Analysis; Tumorigenesis; Xenograft Model Antitumor Assays; Xenografts
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0061664

The motor protein kinesin superfamily proteins (KIFs) are involved in cancer progression. The depletion of one of the KIFs, KIF14, might delay the metaphase-to-anaphase transition, resulting in a binucleated status, which enhances tumor progression; however, the exact correlation between KIF14 and cancer progression remains ambiguous. In this study, using loss of heterozygosity and array comparative genomic hybridization analyses, we observed a 30% loss in the regions surrounding KIF14 on chromosome 1q in lung adenocarcinomas. In addition, the protein expression levels of KIF14 in 122 lung adenocarcinomas also indicated that approximately 30% of adenocarcinomas showed KIF14 down-regulation compared with the expression in the bronchial epithelial cells of adjacent normal counterparts. In addition, the reduced expression of KIF14 mRNA or proteins was correlated with poor overall survival (P = 0.0158 and