Longitudinal MRI evaluation of intracranial development and vascular characteristics of breast cancer brain metastases in a mouse model

Longitudinal MRI was applied to monitor intracranial initiation and development of brain metastases and assess tumor vascular volume and permeability in a mouse model of breast cancer brain metastases. Using a 9.4T system, high resolution anatomic MRI and dynamic susceptibility contrast (DSC) perfus...

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Bibliographic Details
Published in:PloS one 2013-04, Vol.8 (4), p.e62238-e62238
Main Authors: Zhou, Heling, Chen, Min, Zhao, Dawen
Format: Article
Language:English
Subjects:
Animals
Biology
Blood
Blood Vessels - metabolism
Blood Vessels - pathology
Blood volume
Brain
Brain cancer
Brain Neoplasms - blood supply
Brain Neoplasms - diagnosis
Brain Neoplasms - pathology
Brain Neoplasms - secondary
Brain tumors
Breast cancer
Breast Neoplasms - pathology
Cancer
Cell Line, Tumor
Cerebral blood flow
Chemotherapy
Correlation analysis
Diagnostic systems
Disease Models, Animal
Female
Histopathology
Image contrast
Image detection
Injection
Longitudinal Studies
Magnetic Resonance Imaging
Medical prognosis
Medicine
Melanoma
Metastases
Metastasis
Mice
Mice, Nude
Microvasculature
Perfusion
Permeability
Spatial distribution
Studies
Toxicity
Tumors
Ultrasonic imaging
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Summary:Longitudinal MRI was applied to monitor intracranial initiation and development of brain metastases and assess tumor vascular volume and permeability in a mouse model of breast cancer brain metastases. Using a 9.4T system, high resolution anatomic MRI and dynamic susceptibility contrast (DSC) perfusion MRI were acquired at different time points after an intracardiac injection of brain-tropic breast cancer MDA-MB231BR-EGFP cells. Three weeks post injection, multifocal brain metastases were first observed with hyperintensity on T2-weighted images, but isointensity on T1-weighted post contrast images, indicating that blood-tumor-barrier (BTB) at early stage of brain metastases was impermeable. Follow-up MRI revealed intracranial tumor growth and increased number of metastases that distributed throughout the whole brain. At the last scan on week 5, T1-weighted post contrast images detected BTB disruption in 160 (34%) of a total of 464 brain metastases. Enhancement in some of the metastases was only seen in partial regions of the tumor, suggesting intratumoral heterogeneity of BTB disruption. DSC MRI measurements of relative cerebral blood volume (rCBV) showed that rCBV of brain metastases was significantly lower (mean= 0.89±0.03) than that of contralateral normal brain (mean= 1.00±0.03; p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0062238