Structural characterization of neutral and acidic glycolipids from Thermus thermophilus HB8

• Published in: PloS one 2012-07, Vol.7 (7), p.e35067-e35067
• Main Authors: Suda, Yasuo, Okazaki, Fumiaki, Hasegawa, Yushi, Adachi, Seiji, Fukase, Koichi, Kokubo, Susumu, Kuramitsu, Seiki, Kusumoto, Shoichi
• Format: Article
• Language: English
• Subjects: Alkyl groups; Amino acids; Analysis; Bacteria; Bacteroides; Biology; Carbohydrate Sequence; Chemical analysis; Chemical composition; Chemical engineering; Chemistry; Chloroform - chemistry; Chromatography; Chromatography, Thin Layer; Enterococcus hirae; Fatty acids; Glycerol; Glycolipids; Glycolipids - chemistry; Glycolipids - isolation & purification; Hydrogen-Ion Concentration; Lipids; Magnetic resonance; Magnetic Resonance Spectroscopy; Mass Spectrometry; Mass spectroscopy; Membranes; Methanol - chemistry; Molecular Sequence Data; NMR; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Spectroscopy; Structural analysis; Sulfuric acid; Temperature; Thermal stability; Thermus thermophilus; Thermus thermophilus - chemistry
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0035067

The structural characterization of glycolipids from Thermus thermophilus HB8 was performed in this study. Two neutral and one acidic glycolipids were extracted and purified by the modified TLC-blotting method, after which their chemical structures were determined by chemical composition analysis, mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The structure of one of the neutral glycolipids, NGL-A, was Galp(α1-6)GlcpNacyl(β1-2)Glcp(α1-)acyl(2)Gro, and the other, NGL-C, was Galf(β1-2)Galp(α1-6)GlcpNacyl(β1-2)Glcp(α1-)acyl(2)Gro. The structure of NGL-C was identical to that reported previously [Oshima, M. and Ariga, T. (1976) FEBS Lett. 64, 440]. Both neutral glycolipids shared a common structural unit found in the Thermus species. The acyl groups found in NGL-A and NGL-C, iso-type pentadecanoxy and heptadecanoxy fatty acid, were also the same as those found in this species. In contrast, the acidic glycolipid, AGL-B, possessed the structure of N-(((GlcpNAc(α1-)acyl(2)Gro)P-2)GroA)alkylamine. The alkyl group in AGL-B was an iso-type heptadecanyl, suggesting that the iso-type structure of the long alkyl chain is responsible for the thermal stability of the bacteria.