Ultramarine, a chromoprotein acceptor for Förster resonance energy transfer

We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring acti...

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Bibliographic Details
Published in:PloS one 2012-07, Vol.7 (7), p.e41028
Main Authors: Pettikiriarachchi, Anne, Gong, Lan, Perugini, Matthew A, Devenish, Rodney J, Prescott, Mark
Format: Article
Language:English
Subjects:
Amino acids
Apoptosis
Autophagy
Biochemistry
Biology
Biosensors
Caspase
Caspase 3 - metabolism
Caspase-3
Chromatography
Chromatography - methods
Crystal structure
DNA Primers - genetics
Efficiency
Energy
Energy transfer
Entacmaea quadricolor
Enzyme Activation
Fluorescence
Fluorescence Resonance Energy Transfer - instrumentation
Fluorescence Resonance Energy Transfer - methods
Fluorescent Dyes - chemistry
Genomics
Green Fluorescent Proteins - chemistry
HeLa Cells
Humans
Hydrogen-Ion Concentration
Microscopy - methods
Molecular biology
Mutagenesis
Mutagenesis, Site-Directed
Physics
Proteins
Resonance
Sedimentation & deposition
Spectrum analysis
Ultracentrifugation - methods
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Summary:We have engineered a monomeric blue non-fluorescent chromoprotein called Ultramarine (fluorescence quantum yield, 0.001; ε(585 nm), 64,000 M(-1) x cm(-1)) for use as a Förster resonance energy transfer acceptor for a number of different donor fluorescent proteins. We show its use for monitoring activation of caspase 3 in live cells using fluorescence lifetime imaging. Ultramarine has the potential to increase the number of cellular parameters that can be imaged simultaneously.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0041028