Effect of hydrogen peroxide on immersion challenge of rainbow trout fry with Flavobacterium psychrophilum

An experimental model for immersion challenge of rainbow trout fry (Oncorhynchus mykiss) with Flavobacterium psychrophilum, the causative agent of rainbow trout fry syndrome and bacterial cold water disease was established in the present study. Although injection-based infection models are reliable...

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Bibliographic Details
Published in:PloS one 2013-04, Vol.8 (4), p.e62590
Main Authors: Henriksen, Maya Maria Mihályi, Madsen, Lone, Dalsgaard, Inger
Format: Article
Language:English
Subjects:
Agriculture
Animals
Anti-Infective Agents, Local - pharmacology
Bacteria
Biology
Cold water
Earth Sciences
Exposure
Fish
Fish Diseases - microbiology
Flavobacteriaceae Infections - microbiology
Flavobacterium
Flavobacterium - drug effects
Flavobacterium - physiology
Hydrogen
Hydrogen ion concentration
Hydrogen peroxide
Hydrogen Peroxide - pharmacology
Immersion
Laboratory animals
Mortality
Oncorhynchus mykiss
Oncorhynchus mykiss - microbiology
Outbreaks
Pretreatment
Salmon
Trout
Veterinary Science
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Summary:An experimental model for immersion challenge of rainbow trout fry (Oncorhynchus mykiss) with Flavobacterium psychrophilum, the causative agent of rainbow trout fry syndrome and bacterial cold water disease was established in the present study. Although injection-based infection models are reliable and produce high levels of mortality attempts to establish a reproducible immersion model have been less successful. Various concentrations of hydrogen peroxide (H₂O₂) were evaluated before being used as a pre-treatment stressor prior to immersion exposure to F. psychrophilum. H₂O₂ accelerated the onset of mortality and increased mortality approximately two-fold; from 9.1% to 19.2% and from 14.7% to 30.3% in two separate experiments. Clinical signs observed in the infected fish corresponded to symptoms characteristically seen during natural outbreaks. These findings indicate that pre-treatment with H₂O₂ can increase the level of mortality in rainbow trout fry after exposure to F. psychrophilum.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0062590