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A systematic analysis of miRNA transcriptome in Marek's disease virus-induced lymphoma reveals novel and differentially expressed miRNAs
Marek's disease is a lymphoproliferative neoplastic disease of the chicken, which poses a serious threat to poultry health. Marek's disease virus (MDV)-induced T-cell lymphoma is also an excellent biomedical model for neoplasia research. Recently, miRNAs have been demonstrated to play cruc...
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| Published in: | PloS one 2012-11, Vol.7 (11), p.e51003-e51003 |
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| description | Marek's disease is a lymphoproliferative neoplastic disease of the chicken, which poses a serious threat to poultry health. Marek's disease virus (MDV)-induced T-cell lymphoma is also an excellent biomedical model for neoplasia research. Recently, miRNAs have been demonstrated to play crucial roles in mediating neoplastic transformation. To investigate host miRNA expression profiles in the tumor transformation phase of MDV infection, we performed deep sequencing in two MDV-infected samples (tumorous spleen and MD lymphoma from liver), and two non-infected controls (non-infected spleen and lymphocytes). In total, 187 and 16 known miRNAs were identified in chicken and MDV, respectively, and 17 novel chicken miRNAs were further confirmed by qPCR. We identified 28 down-regulated miRNAs and 11 up-regulated miRNAs in MDV-infected samples by bioinformatic analysis. Of nine further tested by qPCR, seven were verified. The gga-miR-181a, gga-miR-26a, gga-miR-221, gga-miR-222, gga-miR-199*, and gga-miR-140* were down-regulated, and gga-miR-146c was up-regulated in MDV-infected tumorous spleens and MD lymphomas. In addition, 189 putative target genes for seven differentially expressed miRNAs were predicted. The luciferase reporter gene assay showed interactions of gga-miR-181a with MYBL1, gga-miR-181a with IGF2BP3, and gga-miR-26a with EIF3A. Differential expression of miRNAs and the predicted targets strongly suggest that they contribute to MDV-induced lymphomagenesis. |
| doi_str_mv | 10.1371/journal.pone.0051003 |
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Marek's disease virus (MDV)-induced T-cell lymphoma is also an excellent biomedical model for neoplasia research. Recently, miRNAs have been demonstrated to play crucial roles in mediating neoplastic transformation. To investigate host miRNA expression profiles in the tumor transformation phase of MDV infection, we performed deep sequencing in two MDV-infected samples (tumorous spleen and MD lymphoma from liver), and two non-infected controls (non-infected spleen and lymphocytes). In total, 187 and 16 known miRNAs were identified in chicken and MDV, respectively, and 17 novel chicken miRNAs were further confirmed by qPCR. We identified 28 down-regulated miRNAs and 11 up-regulated miRNAs in MDV-infected samples by bioinformatic analysis. Of nine further tested by qPCR, seven were verified. The gga-miR-181a, gga-miR-26a, gga-miR-221, gga-miR-222, gga-miR-199*, and gga-miR-140* were down-regulated, and gga-miR-146c was up-regulated in MDV-infected tumorous spleens and MD lymphomas. In addition, 189 putative target genes for seven differentially expressed miRNAs were predicted. The luciferase reporter gene assay showed interactions of gga-miR-181a with MYBL1, gga-miR-181a with IGF2BP3, and gga-miR-26a with EIF3A. Differential expression of miRNAs and the predicted targets strongly suggest that they contribute to MDV-induced lymphomagenesis.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0051003</identifier><identifier>PMID: 23226446</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>3' Untranslated Regions - genetics ; Analysis ; Animal sciences ; Animals ; Base Pairing - genetics ; Base Sequence ; Binding Sites - genetics ; Biology ; Breast cancer ; Breeding of animals ; Cell cycle ; Chickens ; Chickens - genetics ; Chickens - virology ; Engineering ; Gene expression ; Gene Expression Profiling ; Gene Regulatory Networks - genetics ; Genes ; Genes, Reporter ; Genomes ; Health risks ; Herpesvirus 2, Gallid - physiology ; High-Throughput Nucleotide Sequencing ; Infection ; Laboratories ; Liver ; Liver cancer ; Luciferase ; Luciferases - metabolism ; Lymphocytes ; Lymphocytes T ; Lymphoma ; Lymphoma - genetics ; Lymphoma - veterinary ; Lymphoma - virology ; Marek Disease - genetics ; Marek's disease ; Medical research ; Medicine ; Medicine, Experimental ; MicroRNA ; MicroRNAs ; MicroRNAs - genetics ; MicroRNAs - metabolism ; miRNA ; Molecular Sequence Data ; Non-Hodgkin's lymphomas ; Phase transitions ; Poultry Diseases - genetics ; Poultry Diseases - virology ; Reporter gene ; Reproducibility of Results ; Spleen ; Studies ; T cells ; T-cell lymphoma ; Thyroid cancer ; Transcriptome - genetics ; Tumors ; Vaccines ; Veterinary Science ; Viruses ; Zoology</subject><ispartof>PloS one, 2012-11, Vol.7 (11), p.e51003-e51003</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Lian et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2012 Lian et al 2012 Lian et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-27522516a836fb5a64622df1850b325c867c3836d82293f20792364e6372b7e93</citedby><cites>FETCH-LOGICAL-c692t-27522516a836fb5a64622df1850b325c867c3836d82293f20792364e6372b7e93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1351053040/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1351053040?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23226446$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Watson, Michael</contributor><creatorcontrib>Lian, Ling</creatorcontrib><creatorcontrib>Qu, Lujiang</creatorcontrib><creatorcontrib>Chen, Yanmei</creatorcontrib><creatorcontrib>Lamont, Susan J</creatorcontrib><creatorcontrib>Yang, Ning</creatorcontrib><title>A systematic analysis of miRNA transcriptome in Marek's disease virus-induced lymphoma reveals novel and differentially expressed miRNAs</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Marek's disease is a lymphoproliferative neoplastic disease of the chicken, which poses a serious threat to poultry health. Marek's disease virus (MDV)-induced T-cell lymphoma is also an excellent biomedical model for neoplasia research. Recently, miRNAs have been demonstrated to play crucial roles in mediating neoplastic transformation. To investigate host miRNA expression profiles in the tumor transformation phase of MDV infection, we performed deep sequencing in two MDV-infected samples (tumorous spleen and MD lymphoma from liver), and two non-infected controls (non-infected spleen and lymphocytes). In total, 187 and 16 known miRNAs were identified in chicken and MDV, respectively, and 17 novel chicken miRNAs were further confirmed by qPCR. We identified 28 down-regulated miRNAs and 11 up-regulated miRNAs in MDV-infected samples by bioinformatic analysis. Of nine further tested by qPCR, seven were verified. The gga-miR-181a, gga-miR-26a, gga-miR-221, gga-miR-222, gga-miR-199*, and gga-miR-140* were down-regulated, and gga-miR-146c was up-regulated in MDV-infected tumorous spleens and MD lymphomas. In addition, 189 putative target genes for seven differentially expressed miRNAs were predicted. The luciferase reporter gene assay showed interactions of gga-miR-181a with MYBL1, gga-miR-181a with IGF2BP3, and gga-miR-26a with EIF3A. Differential expression of miRNAs and the predicted targets strongly suggest that they contribute to MDV-induced lymphomagenesis.</description><subject>3' Untranslated Regions - genetics</subject><subject>Analysis</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Base Pairing - genetics</subject><subject>Base Sequence</subject><subject>Binding Sites - genetics</subject><subject>Biology</subject><subject>Breast cancer</subject><subject>Breeding of animals</subject><subject>Cell cycle</subject><subject>Chickens</subject><subject>Chickens - genetics</subject><subject>Chickens - virology</subject><subject>Engineering</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Gene Regulatory Networks - genetics</subject><subject>Genes</subject><subject>Genes, Reporter</subject><subject>Genomes</subject><subject>Health risks</subject><subject>Herpesvirus 2, Gallid - physiology</subject><subject>High-Throughput Nucleotide Sequencing</subject><subject>Infection</subject><subject>Laboratories</subject><subject>Liver</subject><subject>Liver cancer</subject><subject>Luciferase</subject><subject>Luciferases - 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Marek's disease virus (MDV)-induced T-cell lymphoma is also an excellent biomedical model for neoplasia research. Recently, miRNAs have been demonstrated to play crucial roles in mediating neoplastic transformation. To investigate host miRNA expression profiles in the tumor transformation phase of MDV infection, we performed deep sequencing in two MDV-infected samples (tumorous spleen and MD lymphoma from liver), and two non-infected controls (non-infected spleen and lymphocytes). In total, 187 and 16 known miRNAs were identified in chicken and MDV, respectively, and 17 novel chicken miRNAs were further confirmed by qPCR. We identified 28 down-regulated miRNAs and 11 up-regulated miRNAs in MDV-infected samples by bioinformatic analysis. Of nine further tested by qPCR, seven were verified. The gga-miR-181a, gga-miR-26a, gga-miR-221, gga-miR-222, gga-miR-199*, and gga-miR-140* were down-regulated, and gga-miR-146c was up-regulated in MDV-infected tumorous spleens and MD lymphomas. In addition, 189 putative target genes for seven differentially expressed miRNAs were predicted. The luciferase reporter gene assay showed interactions of gga-miR-181a with MYBL1, gga-miR-181a with IGF2BP3, and gga-miR-26a with EIF3A. Differential expression of miRNAs and the predicted targets strongly suggest that they contribute to MDV-induced lymphomagenesis.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23226446</pmid><doi>10.1371/journal.pone.0051003</doi><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2012-11, Vol.7 (11), p.e51003-e51003 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1351053040 |
| source | Publicly Available Content (ProQuest); PubMed |
| subjects | 3' Untranslated Regions - genetics Analysis Animal sciences Animals Base Pairing - genetics Base Sequence Binding Sites - genetics Biology Breast cancer Breeding of animals Cell cycle Chickens Chickens - genetics Chickens - virology Engineering Gene expression Gene Expression Profiling Gene Regulatory Networks - genetics Genes Genes, Reporter Genomes Health risks Herpesvirus 2, Gallid - physiology High-Throughput Nucleotide Sequencing Infection Laboratories Liver Liver cancer Luciferase Luciferases - metabolism Lymphocytes Lymphocytes T Lymphoma Lymphoma - genetics Lymphoma - veterinary Lymphoma - virology Marek Disease - genetics Marek's disease Medical research Medicine Medicine, Experimental MicroRNA MicroRNAs MicroRNAs - genetics MicroRNAs - metabolism miRNA Molecular Sequence Data Non-Hodgkin's lymphomas Phase transitions Poultry Diseases - genetics Poultry Diseases - virology Reporter gene Reproducibility of Results Spleen Studies T cells T-cell lymphoma Thyroid cancer Transcriptome - genetics Tumors Vaccines Veterinary Science Viruses Zoology |
| title | A systematic analysis of miRNA transcriptome in Marek's disease virus-induced lymphoma reveals novel and differentially expressed miRNAs |
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