MicroRNA-18a attenuates DNA damage repair through suppressing the expression of ataxia telangiectasia mutated in colorectal cancer

miR-18a is one of the most up-regulated miRNAs in colorectal cancers (CRC) based on miRNA profiling. In this study, we examined the functional significance of miR-18a in CRC. Expression of miR-18a was investigated in 45 CRC patients. Potential target genes of miR-18a were predicted by in silico sear...

Full description

Saved in:
Bibliographic Details
Published in:PloS one 2013-02, Vol.8 (2), p.e57036
Main Authors: Wu, Chung-Wah, Dong, Yu-Juan, Liang, Qiao-Yi, He, Xin-Qi, Ng, Simon S M, Chan, Francis K L, Sung, Joseph J Y, Yu, Jun
Format: Article
Language:English
Subjects:
3' Untranslated Regions
Aged
Aged, 80 and over
Apoptosis
Apoptosis - drug effects
Apoptosis - genetics
Ataxia
Ataxia telangiectasia
Ataxia telangiectasia mutated protein
Ataxia Telangiectasia Mutated Proteins
Base Pairing
Base Sequence
Binding sites
Bioassays
Biology
Bladder cancer
Cancer
Cancer genetics
Cell cycle
Cell Cycle Proteins - genetics
Cell growth
Cell Line, Tumor
Cell survival
Colon
Colon cancer
Colonoscopy
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - genetics
Colorectal Neoplasms - mortality
Colorectal Neoplasms - pathology
Comet assay
Damage accumulation
Damage assessment
Damage detection
Deoxyribonucleic acid
DNA
DNA Breaks, Double-Stranded
DNA damage
DNA Damage - drug effects
DNA methylation
DNA repair
DNA-Binding Proteins - genetics
Ectopic expression
Etoposide
Etoposide - pharmacology
Etoposide - toxicity
Female
Gene expression
Gene Expression Regulation, Neoplastic
Health sciences
Hospitals
Humans
Luciferase
Male
Medical research
Medicine
MicroRNA
MicroRNAs
MicroRNAs - genetics
Middle Aged
miRNA
Neoplasm Staging
Overexpression
Pancreatic cancer
Polymerase chain reaction
Protein-Serine-Threonine Kinases - genetics
Repair
Ribonucleic acid
RNA
RNA Interference
Surgery
Tissues
Toxicity
Tumor Suppressor Proteins - genetics
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:miR-18a is one of the most up-regulated miRNAs in colorectal cancers (CRC) based on miRNA profiling. In this study, we examined the functional significance of miR-18a in CRC. Expression of miR-18a was investigated in 45 CRC patients. Potential target genes of miR-18a were predicted by in silico search and confirmed by luciferase activity assay and Western blot. DNA damage was measured by comet assay. Gene function was measured by cell viability, colony formation and apoptosis assays. The up-regulation of miR-18a was validated and confirmed in 45 primary CRC tumors compared with adjacent normal tissues (p
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0057036