High adhesion of tumor cells to mesothelial monolayers derived from peritoneal wash of disseminated gastrointestinal cancers

• Published in: PloS one 2013-02, Vol.8 (2), p.e57659-e57659
• Main Authors: Ranieri, Danilo, Raffa, Salvatore, Parente, Andrea, Rossi Del Monte, Simone, Ziparo, Vincenzo, Torrisi, Maria Rosaria
• Format: Article
• Language: English
• Subjects: Adhesion; Adhesion tests; Adhesives; Analysis; Antibiotics; Biology; Cancer; Carcinoma - metabolism; Carcinoma - pathology; Cell adhesion; Cell adhesion & migration; Cell Adhesion - genetics; Cell Adhesion - physiology; Cell Line, Tumor; Cell migration; Colon; Colon cancer; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Cytokines; Epithelium - metabolism; Epithelium - pathology; Fluorescence; Gastric cancer; Gastrointestinal cancer; Growth factors; Hospitals; Humans; Intercellular adhesion molecule 1; Intercellular Adhesion Molecule-1 - metabolism; Invasiveness; Medicine; Metastasis; Monolayers; Monomolecular films; Ovarian cancer; Oxidative stress; Patients; Peritoneum; Peritoneum - metabolism; Peritoneum - pathology; Quantitative analysis; Reactive Oxygen Species - metabolism; Senescence; Stomach cancer; Stomach Neoplasms - metabolism; Stomach Neoplasms - pathology; Surgery; Tumor cells; Tumor necrosis factor-TNF; Tumors
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0057659

The role of the mesothelial layer in the peritoneal spreading of cancer cells is only partially clarified. Here we attempted to better define the mesothelial contribution to the tumor cell adhesion using a direct adhesion test applied to human primary cultures of mesothelial cells (HPMCs) derived from the peritoneal washes of patients with gastric and colorectal cancers. Gastric and colon carcinoma cells were seeded on different mesothelial monolayers and quantitative fluorescence analysis was performed to analyze their growth and adhesive properties. The adhesion of the cancer cells was not affected by the origin of the HPMCs when derived from patients with different cancers or with benign disease. In contrast, the high levels of ICAM1 expression and ROS production, which characterize these senescent mesothelial cells, enhanced the tumor cell adhesion. These results suggest that the mesothelial adhesive properties are dependent on the cell senescence, while are not affected by the tumor environment. The use of peritoneal washes as a source to isolate HPMCs provides a practical and reliable tool for the in vitro analysis of the mesothelial conditions affecting the peritoneal carcinomatosis.