Urinary metabolic biomarker and pathway study of hepatitis B virus infected patients based on UPLC-MS system

Hepatitis B virus (HBV) is the fatal consequence of chronic hepatitis, and lack of biomarkers has been a long standing bottleneck in the clinical diagnosis. Metabolomics concerns with comprehensive analysis of small molecules and provides a powerful approach to discover biomarkers in biological syst...

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Published in:PloS one 2013-05, Vol.8 (5), p.e64381-e64381
Main Authors: Zhang, Aihua, Sun, Hui, Han, Ying, Yan, Guangli, Wang, Xijun
Format: Article
Language:English
Subjects:
Adult
Alzheimer's disease
Alzheimers disease
Bioindicators
Biology
Biomarkers
Chemistry
Chromatography
Chromatography, High Pressure Liquid - methods
Diagnosis
Diagnostic systems
Female
Health care
Hepatitis
Hepatitis B
Hepatitis B - urine
Hepatitis B virus
Hepatitis B virus - pathogenicity
Humans
Ionization
Liquid chromatography
Liver cancer
Liver cirrhosis
Male
Mass spectrometry
Mass spectroscopy
Medical diagnosis
Medical research
Medicine
Metabolic pathways
Metabolism
Metabolites
Metabolomics
Metabolomics - methods
Middle Aged
Multivariate statistical analysis
Network analysis
Patient compliance
Patients
Pattern recognition
Permutations
Proteomics
Scientific imaging
Spectrometry, Mass, Electrospray Ionization - methods
Statistical analysis
Stem cells
Urine
Viruses
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creator Zhang, Aihua
Sun, Hui
Han, Ying
Yan, Guangli
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description Hepatitis B virus (HBV) is the fatal consequence of chronic hepatitis, and lack of biomarkers has been a long standing bottleneck in the clinical diagnosis. Metabolomics concerns with comprehensive analysis of small molecules and provides a powerful approach to discover biomarkers in biological systems. Here, we present metabolomics analysis applying ultra-performance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry. (UPLC-Q-TOF-HDMS) to determine metabolite alterations in HBV patients. Most important permutations are elaborated using multivariate statistical analysis and network analysis that was used to select the metabolites for the noninvasive diagnosis of HBV. In this study, the total 11 urinary differential metabolites were identified and contributed to HBV progress involving several key metabolic pathways by using pathway analysis with MetPA, which are promising biomarker candidates for diagnostic research. More importantly, of 11 altered metabolites, 4 metabolite markers were effective for the diagnosis of human HBV, achieved a satisfactory accuracy, sensitivity and specificity, respectively. It demonstrates that metabolomics has the potential as a non-invasive tool to evaluate the potential of these metabolites in the early diagnosis of HBV patients. These findings may be promising to yield a valuable insight into the pathophysiology of HBV and to advance the approaches of diagnosis, treatment, and prevention.
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subjects Adult
Alzheimer's disease
Alzheimers disease
Bioindicators
Biology
Biomarkers
Chemistry
Chromatography
Chromatography, High Pressure Liquid - methods
Diagnosis
Diagnostic systems
Female
Health care
Hepatitis
Hepatitis B
Hepatitis B - urine
Hepatitis B virus
Hepatitis B virus - pathogenicity
Humans
Ionization
Liquid chromatography
Liver cancer
Liver cirrhosis
Male
Mass spectrometry
Mass spectroscopy
Medical diagnosis
Medical research
Medicine
Metabolic pathways
Metabolism
Metabolites
Metabolomics
Metabolomics - methods
Middle Aged
Multivariate statistical analysis
Network analysis
Patient compliance
Patients
Pattern recognition
Permutations
Proteomics
Scientific imaging
Spectrometry, Mass, Electrospray Ionization - methods
Statistical analysis
Stem cells
Urine
Viruses
title Urinary metabolic biomarker and pathway study of hepatitis B virus infected patients based on UPLC-MS system
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