Genome size variation among and within Camellia species by using flow cytometric analysis

The genus Camellia, belonging to the family Theaceae, is economically important group in flowering plants. Frequent interspecific hybridization together with polyploidization has made them become taxonomically "difficult taxa". The DNA content is often used to measure genome size variation...

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Bibliographic Details
Published in:PloS one 2013-05, Vol.8 (5), p.e64981-e64981
Main Authors: Huang, Hui, Tong, Yan, Zhang, Qun-Jie, Gao, Li-Zhi
Format: Article
Language:English
Subjects:
Biodiversity
Biological evolution
Biology
Caffeine
Camellia
Camellia - classification
Camellia - genetics
Coefficient of variation
Cytometry
Deoxyribonucleic acid
DNA
Evolution
Evolution, Molecular
Flow Cytometry
Flowering
Flowers
Genetic Variation
Genome Size
Genome, Plant
Genomes
Genomics
Germplasm
Interspecific
Interspecific hybridization
Iodides
Leaves
Phylogeny
Pinus
Plant sciences
Plant tissues
Ploidies
Polyploidy
Propidium iodide
Studies
Taxa
Variation
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Summary:The genus Camellia, belonging to the family Theaceae, is economically important group in flowering plants. Frequent interspecific hybridization together with polyploidization has made them become taxonomically "difficult taxa". The DNA content is often used to measure genome size variation and has largely advanced our understanding of plant evolution and genome variation. The goals of this study were to investigate patterns of interspecific and intraspecific variation of DNA contents and further explore genome size evolution in a phylogenetic context of the genus. The DNA amount in the genus was determined by using propidium iodide flow cytometry analysis for a total of 139 individual plants representing almost all sections of the two subgenera, Camellia and Thea. An improved WPB buffer was proven to be suitable for the Camellia species, which was able to counteract the negative effects of secondary metabolite and generated high-quality results with low coefficient of variation values (CV)
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0064981