Semen levels of spermatid-specific thioredoxin-3 correlate with pregnancy rates in ART couples

Spermatid specific thioredoxin-3 (SPTRX3 or TXNDC8) is a testis/male germ line specific member of thioredoxin family that accumulates in the superfluous cytoplasm of defective human spermatozoa. We hypothesized that semen levels of SPTRX3 are reflective of treatment outcome in assisted reproductive...

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Bibliographic Details
Published in:PloS one 2013-05, Vol.8 (5), p.e61000-e61000
Main Authors: Buckman, Clayton, Ozanon, Christophe, Qiu, Jing, Sutovsky, Miriam, Carafa, Joyce Ann, Rawe, Vanesa Y, Manandhar, Gaurishankar, Miranda-Vizuete, Antonio, Sutovsky, Peter
Format: Article
Language:English
Subjects:
Adult
Animal sciences
Apoptosis
Bioindicators
Biology
Biomarkers
Biomarkers - metabolism
Blotting, Western
Children
Correlation analysis
Cytometry
Cytoplasm
Data processing
Defects
Dehydrogenases
Deoxyribonucleic acid
DNA
Fatty acids
Female
Fertilization in Vitro
Flow Cytometry
Gynecology
Humans
In vitro fertilization
Infant, Newborn
Infertility
Infertility, Male - metabolism
Infertility, Male - therapy
Laboratories
Male
Medicine
Men
Mens health
Microscopy
Microscopy, Fluorescence
Obstetrics
Oxidative stress
Pregnancy
Pregnancy Outcome
Pregnancy Rate
Purification
Regression Analysis
Reproduction
Reproductive Techniques, Assisted
Semen
Semen - cytology
Semen - metabolism
Semen Analysis
Sperm
Sperm Injections, Intracytoplasmic
Spermatids - metabolism
Spermatozoa
Thioredoxin
Thioredoxins - metabolism
Womens health
Zygotes
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Summary:Spermatid specific thioredoxin-3 (SPTRX3 or TXNDC8) is a testis/male germ line specific member of thioredoxin family that accumulates in the superfluous cytoplasm of defective human spermatozoa. We hypothesized that semen levels of SPTRX3 are reflective of treatment outcome in assisted reproductive therapy (ART) couples treated by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Relationship between SPTRX3 and treatment outcome was investigated in 239 couples undergoing ART at an infertility clinic. Sperm content of SPTRX3 was evaluated by flow cytometry and epifluorescence microscopy, and correlated with clinical semen analysis parameters, and data on embryo development and pregnancy establishment. High SPTRX3 levels (>15% SPTRX3-positive spermatozoa) were found in 51% of male infertility patients (n = 72), in 20% of men from couples with unexplained, idiopathic infertility (n = 61) and in 14% of men from couples previously diagnosed with female-only infertility (n = 85). Couples with high SPTRX3 produced fewer two-pronuclear zygotes and had a reduced pregnancy rate (19.2% pregnant with >15% SPTRX3-positive spermatozoa vs. 41.2% pregnant with
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0061000