Impairment of ribosome maturation or function confers salt resistance on Escherichia coli cells

• Published in: PloS one 2013-05, Vol.8 (5), p.e65747
• Main Authors: Hase, Yoichi, Tarusawa, Takefusa, Muto, Akira, Himeno, Hyouta
• Format: Article
• Language: English
• Subjects: Abiotic stress; Aminoglycosides - pharmacology; Anti-Bacterial Agents - pharmacology; Bacteria; Biochemistry; Biology; Biosynthesis; Chloramphenicol; DNA - biosynthesis; DNA Replication - drug effects; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Ethanol; Gene Expression Regulation, Bacterial - drug effects; Kasugamycin; Life sciences; Maturation; Molecular biology; Mutation; Phenols; Potassium; Protein biosynthesis; Protein synthesis; Proteins; Ribosomes - metabolism; RNA polymerase; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 16S - metabolism; rRNA 16S; Salt tolerance; Salt-Tolerance - genetics; Salts; Sigma factor; Sigma Factor - metabolism; Stress, Physiological - genetics
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0065747

We found that loss of integrity of the ribosome by removal of a putative ribosome maturation factor or a ribosomal protein conferred salt tolerance on Escherichia coli cells. Some protein synthesis inhibitors including kasugamycin and chloramphenicol also had a similar effect, although kasugamycin affected neither 16S rRNA maturation nor subunit association into a 70S ribosome. Thus, salt tolerance is a common feature of cells in which maturation or function of the ribosome is impaired. In these cells, premature induction of an alternative sigma factor, σ(E), by salt stress was observed. These results suggest the existence of a yet-unknown stress response pathway mediated by the bacterial ribosome.