p38 signaling and receptor recycling events in a microfluidic endothelial cell adhesion assay

Adhesion-based microfluidic cell separation has proven to be very useful in applications ranging from cancer diagnostics to tissue engineering. This process involves functionalizing microchannel surfaces with a capture molecule. High specificity and purity capture can be achieved using this method....

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Bibliographic Details
Published in:PloS one 2013-06, Vol.8 (6), p.e65828-e65828
Main Authors: Vickers, Dwayne A L, Chory, Emma J, Harless, Megan C, Murthy, Shashi K
Format: Article
Language:English
Subjects:
Adhesion
Biology
Cancer
Cell Adhesion
Cell adhesion & migration
Chemical engineering
Curing
Endocytosis
Endothelial cells
Endothelium
Engineering
Ethanol
Fluid flow
Human Umbilical Vein Endothelial Cells - cytology
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Integrin beta1 - metabolism
Kinases
MAP kinase
MAP Kinase Signaling System
Mechanical stimuli
Microchannels
Microfluidics
Microfluidics - methods
p38 Mitogen-Activated Protein Kinases - antagonists & inhibitors
p38 Mitogen-Activated Protein Kinases - metabolism
Peptides - metabolism
Physics
Process parameters
Protein kinase
Protein turnover
Receptors
Rheology
Shear stress
Signaling
Silicon wafers
Solubility
Stress, Mechanical
Tissue engineering
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Summary:Adhesion-based microfluidic cell separation has proven to be very useful in applications ranging from cancer diagnostics to tissue engineering. This process involves functionalizing microchannel surfaces with a capture molecule. High specificity and purity capture can be achieved using this method. Despite these advances, little is known about the mechanisms that govern cell capture within these devices and their relationships to basic process parameters such as fluid shear stress and the presence of soluble factors. This work examines how the adhesion of human endothelial cells (ECs) is influenced by a soluble tetrapeptide, Arg-Glu-Asp-Val (REDV) and fluidic shear stress. The ability of these ECs to bind within microchannels coated with REDV is shown to be governed by shear- and soluble-factor mediated changes in p38 mitogen-activated protein kinase expression together with recycling of adhesion receptors from the endosome.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0065828