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Gamete therapeutics: recombinant protein adsorption by sperm for increasing fertility via artificial insemination

A decrease in fertility can have a negative economic impact, both locally and over a broader geographical scope, and this is especially the case with regard to the cattle industry. Therefore, much interest exists in evaluating proteins that might be able to increase the fertility of sperm. Heparin b...

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Published in:PloS one 2013-06, Vol.8 (6), p.e65083-e65083
Main Authors: Alvarez-Gallardo, Horacio, Kjelland, Michael E, Moreno, Juan F, Welsh, Jr, Thomas H, Randel, Ronald D, Lammoglia, Miguel A, Pérez-Martínez, Mario, Lara-Sagahón, Alma V, Esperón-Sumano, A Enrique, Romo, Salvador
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cited_by cdi_FETCH-LOGICAL-c692t-f5d14f6d3c7a12129f886089ed4715c1ad2e4b3002ab83298db2312872e919bd3
cites cdi_FETCH-LOGICAL-c692t-f5d14f6d3c7a12129f886089ed4715c1ad2e4b3002ab83298db2312872e919bd3
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container_title PloS one
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creator Alvarez-Gallardo, Horacio
Kjelland, Michael E
Moreno, Juan F
Welsh, Jr, Thomas H
Randel, Ronald D
Lammoglia, Miguel A
Pérez-Martínez, Mario
Lara-Sagahón, Alma V
Esperón-Sumano, A Enrique
Romo, Salvador
description A decrease in fertility can have a negative economic impact, both locally and over a broader geographical scope, and this is especially the case with regard to the cattle industry. Therefore, much interest exists in evaluating proteins that might be able to increase the fertility of sperm. Heparin binding proteins (HBPs), specifically the fertility associated antigen (FAA) and the Type-2 tissue inhibitor of metalloproteinase (TIMP-2), act to favor the capacitation and acrosome reaction and perhaps even modulate the immune system's response toward the sperm. The objective of this research was to determine the effect on fertility of adding recombinant FAA (rFAA) and recombinant TIMP-2 (rTIMP-2) to bovine semen before cryopreservation for use in an artificial insemination (AI) program in a tropical environment. For this experiment, 100 crossbred (Bos taurus x Bos indicus) heifers were selected based on their estrus cycle, body condition score (BCS), of 4 to 6 on a scale of 1 to 9, and adequate anatomical conformation evaluated by pelvic and genital (normal) measurements. Heifers were synchronized using estradiol benzoate (EB), Celosil® (PGF2α) (Shering-Plough) and a controlled internal drug release (CIDR) device was inserted that contained progesterone. Inseminations were performed in two groups at random, 50 animals per group. The control group was inseminated with conventional semen. The treatment group was inseminated with semen containing rFAA (25 µg/mL) and rTIMP-2 (25 µg/mL). In the control group a 16% pregnancy rate was obtained versus a 40% pregnancy rate for the HBP treatment group, resulting in a significant difference (P = 0.0037). Given the results herein, one may conclude that the HBPs can increase fertility and could be an option for cattle in tropical conditions; however, one needs to consider the environment, nutrition, and the genetic interaction affecting the final result in whatever reproductive program that is implemented.
doi_str_mv 10.1371/journal.pone.0065083
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Therefore, much interest exists in evaluating proteins that might be able to increase the fertility of sperm. Heparin binding proteins (HBPs), specifically the fertility associated antigen (FAA) and the Type-2 tissue inhibitor of metalloproteinase (TIMP-2), act to favor the capacitation and acrosome reaction and perhaps even modulate the immune system's response toward the sperm. The objective of this research was to determine the effect on fertility of adding recombinant FAA (rFAA) and recombinant TIMP-2 (rTIMP-2) to bovine semen before cryopreservation for use in an artificial insemination (AI) program in a tropical environment. For this experiment, 100 crossbred (Bos taurus x Bos indicus) heifers were selected based on their estrus cycle, body condition score (BCS), of 4 to 6 on a scale of 1 to 9, and adequate anatomical conformation evaluated by pelvic and genital (normal) measurements. Heifers were synchronized using estradiol benzoate (EB), Celosil® (PGF2α) (Shering-Plough) and a controlled internal drug release (CIDR) device was inserted that contained progesterone. Inseminations were performed in two groups at random, 50 animals per group. The control group was inseminated with conventional semen. The treatment group was inseminated with semen containing rFAA (25 µg/mL) and rTIMP-2 (25 µg/mL). In the control group a 16% pregnancy rate was obtained versus a 40% pregnancy rate for the HBP treatment group, resulting in a significant difference (P = 0.0037). Given the results herein, one may conclude that the HBPs can increase fertility and could be an option for cattle in tropical conditions; however, one needs to consider the environment, nutrition, and the genetic interaction affecting the final result in whatever reproductive program that is implemented.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23762288</pmid><doi>10.1371/journal.pone.0065083</doi><tpages>e65083</tpages><oa>free_for_read</oa></addata></record>
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identifier ISSN: 1932-6203
ispartof PloS one, 2013-06, Vol.8 (6), p.e65083-e65083
issn 1932-6203
1932-6203
language eng
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source PubMed Central(OpenAccess); Publicly Available Content (ProQuest)
subjects 17β-Estradiol
Acrosome reaction
Adsorption
Agriculture
Animals
Anticoagulants
Artificial insemination
Beef
Binding proteins
Biology
Bovidae
Capacitation
Cattle
Cryopreservation
Drug delivery systems
Economic impact
Estrus cycle
Estrus Synchronization
Female
Fertility
Fertility - physiology
Fluorescent Antibody Technique, Indirect
Heparin
Humans
Immune system
Impact analysis
Insemination, Artificial
Laboratory animals
Male
Males
Membrane Proteins - administration & dosage
Membrane Proteins - metabolism
Metalloproteinase
Nutrition
Ovulation
Plasma
Pregnancy
Pregnancy Rate
Progesterone
Prostate
Protein adsorption
Protein binding
Proteins
Recombinant proteins
Recombinant Proteins - administration & dosage
Recombinant Proteins - metabolism
Reproduction (biology)
Semen
Semen - metabolism
Sex hormones
Sperm
Spermatozoa - metabolism
Tissue inhibitor of metalloproteinase 2
Tissue Inhibitor of Metalloproteinase-2 - administration & dosage
Tissue Inhibitor of Metalloproteinase-2 - metabolism
Tropical environment
Tropical environments
Veterinary Science
title Gamete therapeutics: recombinant protein adsorption by sperm for increasing fertility via artificial insemination
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