Loading…
Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo
Tantalum, as a potential metallic implant biomaterial, is attracting more and more attention because of its excellent anticorrosion and biocompatibility. However, its significantly high elastic modulus and large mechanical incompatibility with bone tissue make it unsuitable for load-bearing implants...
Saved in:
| Published in: | PloS one 2013-06, Vol.8 (6), p.e66263-e66263 |
|---|---|
| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-c692t-cf4392199494799d3688123ef6412f77ddca09152bcb02e8cc8f7201fa8cdb3 |
|---|---|
| cites | cdi_FETCH-LOGICAL-c692t-cf4392199494799d3688123ef6412f77ddca09152bcb02e8cc8f7201fa8cdb3 |
| container_end_page | e66263 |
| container_issue | 6 |
| container_start_page | e66263 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Tang, Ze Xie, Youtao Yang, Fei Huang, Yan Wang, Chuandong Dai, Kerong Zheng, Xuebin Zhang, Xiaoling |
| description | Tantalum, as a potential metallic implant biomaterial, is attracting more and more attention because of its excellent anticorrosion and biocompatibility. However, its significantly high elastic modulus and large mechanical incompatibility with bone tissue make it unsuitable for load-bearing implants. In this study, porous tantalum coatings were first successfully fabricated on titanium substrates by vacuum plasma spraying (VPS), which would exert the excellent biocompatibility of tantalum and alleviate the elastic modulus of tantalum for bone tissue. We evaluated cytocompatibility and osteogenesis activity of the porous tantalum coatings using human bone marrow stromal cells (hBMSCs) and its ability to repair rabbit femur bone defects. The morphology and actin cytoskeletons of hBMSCs were observed via electron microscopy and confocal, and the cell viability, proliferation and osteogenic differentiation potential of hBMSCs were examined quantitatively by PrestoBlue assay, Ki67 immunofluorescence assay, real-time PCR technology and ALP staining. For in vivo detection, the repaired femur were evaluated by histomorphology and double fluorescence labeling 3 months postoperation. Porous tantalum coating surfaces promoted hBMSCs adhesion, proliferation, osteogenesis activity and had better osseointegration and faster new bone formation rate than titanium coating control. Our observation suggested that the porous tantalum coatings had good biocompatibility and could enhance osseoinductivity in vitro and promote new bone formation in vivo. The porous tantalum coatings prepared by VPS is a promising strategy for bone regeneration. |
| doi_str_mv | 10.1371/journal.pone.0066263 |
| format | article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1366648199</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478325818</galeid><doaj_id>oai_doaj_org_article_09edf12614f8425e8be9b0e61d0ab6f1</doaj_id><sourcerecordid>A478325818</sourcerecordid><originalsourceid>FETCH-LOGICAL-c692t-cf4392199494799d3688123ef6412f77ddca09152bcb02e8cc8f7201fa8cdb3</originalsourceid><addsrcrecordid>eNqNk81u1DAQxyMEoqXwBggsISE47OKPrGNfkKqKj5UqFVHE1XKc8a6rxA52sqJPwSvj7G6rXdQDyiHxzG_-M57JFMVLgueEVeTDTRij1-28Dx7mGHNOOXtUnBLJ6IxTzB4ffJ8Uz1K6wXjBBOdPixPKqorzUpwWf76FGMaEBu0H3Y4dMkEPzq8S6iP0OkKD6lu00WbMvr7VqdMo9VHfZgaBX2tvANVdMgmFNEBYgXcGNc5aiOAHl8WCR9pnmVwnipABiDur82jjhhi27u1hE54XT6xuE7zYv8-K68-fflx8nV1efVlenF_ODJd0mBlbMkmJlKUsKykbxoUglIHlJaG2qprGaCzJgtamxhSEMcJWFBOrhWlqdla83qn2bUhq38mkCONTV7JsJpY7ogn6RvXRdTreqqCd2hpCXCkdB2daUFhCYwnlpLSipAsQNcgaAycN1jW3JGt93Gcb6w4ak_sSdXskeuzxbq1WYaMYryQup2Le7QVi-DVCGlTnkoG21R7y8Ka6ZUWExFOuN_-gD99uT610voDzNuS8ZhJV52UlGF0IIjI1f4DKTwOdM3mc1mX7UcD7o4DMDPB7WOkxJbW8_v7_7NXPY_btAbsG3Q7rFNpx-ovSMVjuQBNDShHsfZMJVtPW3HVDTVuj9luTw14dDug-6G5N2F9RjBVd</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1366648199</pqid></control><display><type>article</type><title>Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo</title><source>Open Access: PubMed Central</source><source>ProQuest Publicly Available Content database</source><creator>Tang, Ze ; Xie, Youtao ; Yang, Fei ; Huang, Yan ; Wang, Chuandong ; Dai, Kerong ; Zheng, Xuebin ; Zhang, Xiaoling</creator><contributor>Tuckermann, Jan Peter</contributor><creatorcontrib>Tang, Ze ; Xie, Youtao ; Yang, Fei ; Huang, Yan ; Wang, Chuandong ; Dai, Kerong ; Zheng, Xuebin ; Zhang, Xiaoling ; Tuckermann, Jan Peter</creatorcontrib><description>Tantalum, as a potential metallic implant biomaterial, is attracting more and more attention because of its excellent anticorrosion and biocompatibility. However, its significantly high elastic modulus and large mechanical incompatibility with bone tissue make it unsuitable for load-bearing implants. In this study, porous tantalum coatings were first successfully fabricated on titanium substrates by vacuum plasma spraying (VPS), which would exert the excellent biocompatibility of tantalum and alleviate the elastic modulus of tantalum for bone tissue. We evaluated cytocompatibility and osteogenesis activity of the porous tantalum coatings using human bone marrow stromal cells (hBMSCs) and its ability to repair rabbit femur bone defects. The morphology and actin cytoskeletons of hBMSCs were observed via electron microscopy and confocal, and the cell viability, proliferation and osteogenic differentiation potential of hBMSCs were examined quantitatively by PrestoBlue assay, Ki67 immunofluorescence assay, real-time PCR technology and ALP staining. For in vivo detection, the repaired femur were evaluated by histomorphology and double fluorescence labeling 3 months postoperation. Porous tantalum coating surfaces promoted hBMSCs adhesion, proliferation, osteogenesis activity and had better osseointegration and faster new bone formation rate than titanium coating control. Our observation suggested that the porous tantalum coatings had good biocompatibility and could enhance osseoinductivity in vitro and promote new bone formation in vivo. The porous tantalum coatings prepared by VPS is a promising strategy for bone regeneration.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0066263</identifier><identifier>PMID: 23776648</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Actin ; Animals ; Biocompatibility ; Biological products ; Biology ; Biomedical materials ; Bone growth ; Bone implants ; Bone marrow ; Bone Regeneration - drug effects ; Bone surgery ; Cell Adhesion - drug effects ; Cell Differentiation - drug effects ; Cell Proliferation - drug effects ; Cells, Cultured ; Ceramics ; Coatings ; Confocal ; Corrosion prevention ; Defects ; Differentiation ; Electron microscopy ; Femur ; Fluorescence ; Health sciences ; Hospitals ; Humans ; Immunofluorescence ; Incompatibility ; Joint surgery ; Laboratory animals ; Materials Science ; Mathematics ; Mechanical properties ; Medical practices ; Medicine ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - drug effects ; Modulus of elasticity ; Osseointegration ; Osteogenesis ; Osteogenesis - drug effects ; Plasma spraying ; Porosity ; Prostheses and Implants ; Protective coatings ; Rabbits ; Real-Time Polymerase Chain Reaction ; Regeneration ; Regeneration (physiology) ; Spraying ; Stem cells ; Stromal cells ; Studies ; Substrates ; Surgical implants ; Tantalum ; Tantalum - chemistry ; Tantalum - pharmacology ; Tissue Engineering ; Titanium ; Transplants & implants ; Vacuum ; Vacuum plasma spraying</subject><ispartof>PloS one, 2013-06, Vol.8 (6), p.e66263-e66263</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Tang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Tang et al 2013 Tang et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-cf4392199494799d3688123ef6412f77ddca09152bcb02e8cc8f7201fa8cdb3</citedby><cites>FETCH-LOGICAL-c692t-cf4392199494799d3688123ef6412f77ddca09152bcb02e8cc8f7201fa8cdb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1366648199/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1366648199?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23776648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Tuckermann, Jan Peter</contributor><creatorcontrib>Tang, Ze</creatorcontrib><creatorcontrib>Xie, Youtao</creatorcontrib><creatorcontrib>Yang, Fei</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Wang, Chuandong</creatorcontrib><creatorcontrib>Dai, Kerong</creatorcontrib><creatorcontrib>Zheng, Xuebin</creatorcontrib><creatorcontrib>Zhang, Xiaoling</creatorcontrib><title>Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Tantalum, as a potential metallic implant biomaterial, is attracting more and more attention because of its excellent anticorrosion and biocompatibility. However, its significantly high elastic modulus and large mechanical incompatibility with bone tissue make it unsuitable for load-bearing implants. In this study, porous tantalum coatings were first successfully fabricated on titanium substrates by vacuum plasma spraying (VPS), which would exert the excellent biocompatibility of tantalum and alleviate the elastic modulus of tantalum for bone tissue. We evaluated cytocompatibility and osteogenesis activity of the porous tantalum coatings using human bone marrow stromal cells (hBMSCs) and its ability to repair rabbit femur bone defects. The morphology and actin cytoskeletons of hBMSCs were observed via electron microscopy and confocal, and the cell viability, proliferation and osteogenic differentiation potential of hBMSCs were examined quantitatively by PrestoBlue assay, Ki67 immunofluorescence assay, real-time PCR technology and ALP staining. For in vivo detection, the repaired femur were evaluated by histomorphology and double fluorescence labeling 3 months postoperation. Porous tantalum coating surfaces promoted hBMSCs adhesion, proliferation, osteogenesis activity and had better osseointegration and faster new bone formation rate than titanium coating control. Our observation suggested that the porous tantalum coatings had good biocompatibility and could enhance osseoinductivity in vitro and promote new bone formation in vivo. The porous tantalum coatings prepared by VPS is a promising strategy for bone regeneration.</description><subject>Actin</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biological products</subject><subject>Biology</subject><subject>Biomedical materials</subject><subject>Bone growth</subject><subject>Bone implants</subject><subject>Bone marrow</subject><subject>Bone Regeneration - drug effects</subject><subject>Bone surgery</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cells, Cultured</subject><subject>Ceramics</subject><subject>Coatings</subject><subject>Confocal</subject><subject>Corrosion prevention</subject><subject>Defects</subject><subject>Differentiation</subject><subject>Electron microscopy</subject><subject>Femur</subject><subject>Fluorescence</subject><subject>Health sciences</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Immunofluorescence</subject><subject>Incompatibility</subject><subject>Joint surgery</subject><subject>Laboratory animals</subject><subject>Materials Science</subject><subject>Mathematics</subject><subject>Mechanical properties</subject><subject>Medical practices</subject><subject>Medicine</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - drug effects</subject><subject>Modulus of elasticity</subject><subject>Osseointegration</subject><subject>Osteogenesis</subject><subject>Osteogenesis - drug effects</subject><subject>Plasma spraying</subject><subject>Porosity</subject><subject>Prostheses and Implants</subject><subject>Protective coatings</subject><subject>Rabbits</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Regeneration</subject><subject>Regeneration (physiology)</subject><subject>Spraying</subject><subject>Stem cells</subject><subject>Stromal cells</subject><subject>Studies</subject><subject>Substrates</subject><subject>Surgical implants</subject><subject>Tantalum</subject><subject>Tantalum - chemistry</subject><subject>Tantalum - pharmacology</subject><subject>Tissue Engineering</subject><subject>Titanium</subject><subject>Transplants & implants</subject><subject>Vacuum</subject><subject>Vacuum plasma spraying</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNqNk81u1DAQxyMEoqXwBggsISE47OKPrGNfkKqKj5UqFVHE1XKc8a6rxA52sqJPwSvj7G6rXdQDyiHxzG_-M57JFMVLgueEVeTDTRij1-28Dx7mGHNOOXtUnBLJ6IxTzB4ffJ8Uz1K6wXjBBOdPixPKqorzUpwWf76FGMaEBu0H3Y4dMkEPzq8S6iP0OkKD6lu00WbMvr7VqdMo9VHfZgaBX2tvANVdMgmFNEBYgXcGNc5aiOAHl8WCR9pnmVwnipABiDur82jjhhi27u1hE54XT6xuE7zYv8-K68-fflx8nV1efVlenF_ODJd0mBlbMkmJlKUsKykbxoUglIHlJaG2qprGaCzJgtamxhSEMcJWFBOrhWlqdla83qn2bUhq38mkCONTV7JsJpY7ogn6RvXRdTreqqCd2hpCXCkdB2daUFhCYwnlpLSipAsQNcgaAycN1jW3JGt93Gcb6w4ak_sSdXskeuzxbq1WYaMYryQup2Le7QVi-DVCGlTnkoG21R7y8Ka6ZUWExFOuN_-gD99uT610voDzNuS8ZhJV52UlGF0IIjI1f4DKTwOdM3mc1mX7UcD7o4DMDPB7WOkxJbW8_v7_7NXPY_btAbsG3Q7rFNpx-ovSMVjuQBNDShHsfZMJVtPW3HVDTVuj9luTw14dDug-6G5N2F9RjBVd</recordid><startdate>20130611</startdate><enddate>20130611</enddate><creator>Tang, Ze</creator><creator>Xie, Youtao</creator><creator>Yang, Fei</creator><creator>Huang, Yan</creator><creator>Wang, Chuandong</creator><creator>Dai, Kerong</creator><creator>Zheng, Xuebin</creator><creator>Zhang, Xiaoling</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130611</creationdate><title>Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo</title><author>Tang, Ze ; Xie, Youtao ; Yang, Fei ; Huang, Yan ; Wang, Chuandong ; Dai, Kerong ; Zheng, Xuebin ; Zhang, Xiaoling</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-cf4392199494799d3688123ef6412f77ddca09152bcb02e8cc8f7201fa8cdb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Actin</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biological products</topic><topic>Biology</topic><topic>Biomedical materials</topic><topic>Bone growth</topic><topic>Bone implants</topic><topic>Bone marrow</topic><topic>Bone Regeneration - drug effects</topic><topic>Bone surgery</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cells, Cultured</topic><topic>Ceramics</topic><topic>Coatings</topic><topic>Confocal</topic><topic>Corrosion prevention</topic><topic>Defects</topic><topic>Differentiation</topic><topic>Electron microscopy</topic><topic>Femur</topic><topic>Fluorescence</topic><topic>Health sciences</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Immunofluorescence</topic><topic>Incompatibility</topic><topic>Joint surgery</topic><topic>Laboratory animals</topic><topic>Materials Science</topic><topic>Mathematics</topic><topic>Mechanical properties</topic><topic>Medical practices</topic><topic>Medicine</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - drug effects</topic><topic>Modulus of elasticity</topic><topic>Osseointegration</topic><topic>Osteogenesis</topic><topic>Osteogenesis - drug effects</topic><topic>Plasma spraying</topic><topic>Porosity</topic><topic>Prostheses and Implants</topic><topic>Protective coatings</topic><topic>Rabbits</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Regeneration</topic><topic>Regeneration (physiology)</topic><topic>Spraying</topic><topic>Stem cells</topic><topic>Stromal cells</topic><topic>Studies</topic><topic>Substrates</topic><topic>Surgical implants</topic><topic>Tantalum</topic><topic>Tantalum - chemistry</topic><topic>Tantalum - pharmacology</topic><topic>Tissue Engineering</topic><topic>Titanium</topic><topic>Transplants & implants</topic><topic>Vacuum</topic><topic>Vacuum plasma spraying</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tang, Ze</creatorcontrib><creatorcontrib>Xie, Youtao</creatorcontrib><creatorcontrib>Yang, Fei</creatorcontrib><creatorcontrib>Huang, Yan</creatorcontrib><creatorcontrib>Wang, Chuandong</creatorcontrib><creatorcontrib>Dai, Kerong</creatorcontrib><creatorcontrib>Zheng, Xuebin</creatorcontrib><creatorcontrib>Zhang, Xiaoling</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints database</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Complete (ProQuest Database)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agriculture Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials science collection</collection><collection>ProQuest Publicly Available Content database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tang, Ze</au><au>Xie, Youtao</au><au>Yang, Fei</au><au>Huang, Yan</au><au>Wang, Chuandong</au><au>Dai, Kerong</au><au>Zheng, Xuebin</au><au>Zhang, Xiaoling</au><au>Tuckermann, Jan Peter</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-06-11</date><risdate>2013</risdate><volume>8</volume><issue>6</issue><spage>e66263</spage><epage>e66263</epage><pages>e66263-e66263</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Tantalum, as a potential metallic implant biomaterial, is attracting more and more attention because of its excellent anticorrosion and biocompatibility. However, its significantly high elastic modulus and large mechanical incompatibility with bone tissue make it unsuitable for load-bearing implants. In this study, porous tantalum coatings were first successfully fabricated on titanium substrates by vacuum plasma spraying (VPS), which would exert the excellent biocompatibility of tantalum and alleviate the elastic modulus of tantalum for bone tissue. We evaluated cytocompatibility and osteogenesis activity of the porous tantalum coatings using human bone marrow stromal cells (hBMSCs) and its ability to repair rabbit femur bone defects. The morphology and actin cytoskeletons of hBMSCs were observed via electron microscopy and confocal, and the cell viability, proliferation and osteogenic differentiation potential of hBMSCs were examined quantitatively by PrestoBlue assay, Ki67 immunofluorescence assay, real-time PCR technology and ALP staining. For in vivo detection, the repaired femur were evaluated by histomorphology and double fluorescence labeling 3 months postoperation. Porous tantalum coating surfaces promoted hBMSCs adhesion, proliferation, osteogenesis activity and had better osseointegration and faster new bone formation rate than titanium coating control. Our observation suggested that the porous tantalum coatings had good biocompatibility and could enhance osseoinductivity in vitro and promote new bone formation in vivo. The porous tantalum coatings prepared by VPS is a promising strategy for bone regeneration.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23776648</pmid><doi>10.1371/journal.pone.0066263</doi><tpages>e66263</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-06, Vol.8 (6), p.e66263-e66263 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1366648199 |
| source | Open Access: PubMed Central; ProQuest Publicly Available Content database |
| subjects | Actin Animals Biocompatibility Biological products Biology Biomedical materials Bone growth Bone implants Bone marrow Bone Regeneration - drug effects Bone surgery Cell Adhesion - drug effects Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured Ceramics Coatings Confocal Corrosion prevention Defects Differentiation Electron microscopy Femur Fluorescence Health sciences Hospitals Humans Immunofluorescence Incompatibility Joint surgery Laboratory animals Materials Science Mathematics Mechanical properties Medical practices Medicine Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - drug effects Modulus of elasticity Osseointegration Osteogenesis Osteogenesis - drug effects Plasma spraying Porosity Prostheses and Implants Protective coatings Rabbits Real-Time Polymerase Chain Reaction Regeneration Regeneration (physiology) Spraying Stem cells Stromal cells Studies Substrates Surgical implants Tantalum Tantalum - chemistry Tantalum - pharmacology Tissue Engineering Titanium Transplants & implants Vacuum Vacuum plasma spraying |
| title | Porous tantalum coatings prepared by vacuum plasma spraying enhance bmscs osteogenic differentiation and bone regeneration in vitro and in vivo |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T19%3A27%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Porous%20tantalum%20coatings%20prepared%20by%20vacuum%20plasma%20spraying%20enhance%20bmscs%20osteogenic%20differentiation%20and%20bone%20regeneration%20in%20vitro%20and%20in%20vivo&rft.jtitle=PloS%20one&rft.au=Tang,%20Ze&rft.date=2013-06-11&rft.volume=8&rft.issue=6&rft.spage=e66263&rft.epage=e66263&rft.pages=e66263-e66263&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0066263&rft_dat=%3Cgale_plos_%3EA478325818%3C/gale_plos_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c692t-cf4392199494799d3688123ef6412f77ddca09152bcb02e8cc8f7201fa8cdb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1366648199&rft_id=info:pmid/23776648&rft_galeid=A478325818&rfr_iscdi=true |