Gibberellin biosynthetic deficiency is responsible for maize dominant Dwarf11 (D11) mutant phenotype: physiological and transcriptomic evidence

• Published in: PloS one 2013-06, Vol.8 (6), p.e66466-e66466
• Main Authors: Wang, Yijun, Deng, Dexiang, Ding, Haidong, Xu, Xiangming, Zhang, Rong, Wang, Suxin, Bian, Yunlong, Yin, Zhitong, Chen, Yao
• Format: Article
• Language: English
• Subjects: Abnormalities; Agricultural production; Agriculture; Analysis; Arabidopsis; Base Sequence; Biology; Biosynthesis; Biosynthetic Pathways - genetics; Breeding; Breeding - methods; Cereal crops; Cloning; Cloning, Molecular; Corn; Crop production; Crops; Cytochrome; DNA microarrays; DNA Primers - genetics; Dwarfism; Education; Enzymes; GA 20-oxidase; Gene Expression Profiling; Genes; Genetic aspects; Genetics; Genomes; Genomics; Genotype & phenotype; Gibberellins - biosynthesis; Gibberellins - pharmacology; Laboratories; Lodging; Metabolism; Microarray Analysis; Mixed Function Oxygenases - genetics; Molecular Sequence Data; Mutation; Oryza; Oryza sativa; Oxidase; Oxidases; Phenotype; Phenotypes; Physiology; Plant breeding; Plants (botany); Real-Time Polymerase Chain Reaction; Rice; Seedlings; Sequence Analysis, DNA; Signaling; Species Specificity; Transcription factors; Triazoles - pharmacology; Wheat; Zea mays - genetics; Zea mays - growth & development
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0066466

Dwarf stature is introduced to improve lodging resistance and harvest index in crop production. In many crops including maize, mining and application of novel dwarf genes are urgent to overcome genetic bottleneck and vulnerability during breeding improvement. Here we report the characterization and expression profiling analysis of a newly identified maize dwarf mutant Dwarf11 (D11). The D11 displays severely developmental abnormalities and is controlled by a dominant Mendelian factor. The D11 seedlings responds to both GA3 and paclobutrazol (PAC) application, suggesting that dwarf phenotype of D11 is caused by GA biosynthesis instead of GA signaling deficiency. In contrast, two well-characterized maize dominant dwarf plants D8 and D9 are all insensitive to exogenous GA3 stimulation. Additionally, sequence variation of D8 and D9 genes was not identified in the D11 mutant. Microarray and qRT-PCR analysis results demonstrated that transcripts encoding GA biosynthetic and catabolic enzymes ent-kaurenoic acid oxidase (KAO), GA 20-oxidase (GA20ox), and GA 2-oxidase (GA2ox) are up-regulated in D11. Our results lay a foundation for the following D11 gene cloning and functional characterization. Moreover, results presented here may aid in crops molecular improvement and breeding, especially breeding of crops with plant height ideotypes.