Cell polarity and patterning by PIN trafficking through early endosomal compartments in Arabidopsis thaliana

PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of the plant hormone auxin that is crucial for a multitude of developmental processes in plants. PIN localization is under extensive control by environmental or developmental cues, but...

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Published in:PLoS genetics 2013-05, Vol.9 (5), p.e1003540-e1003540
Main Authors: Tanaka, Hirokazu, Kitakura, Saeko, Rakusová, Hana, Uemura, Tomohiro, Feraru, Mugurel I, De Rycke, Riet, Robert, Stéphanie, Kakimoto, Tatsuo, Friml, Jiří
Format: Article
Language:English
Subjects:
Alcohol Oxidoreductases - genetics
Alcohol Oxidoreductases - metabolism
Arabidopsis - genetics
Arabidopsis - growth & development
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Biological Transport
Biology
Cell Membrane - genetics
Cell Membrane - metabolism
Cell Polarity - genetics
Cytoplasmic Dyneins - genetics
Defects
Developmental biology
Embryonic development
Endosomes - genetics
Endosomes - metabolism
Flowers & plants
Gene Expression Regulation, Plant
Genetic aspects
Indoleacetic Acids - metabolism
Munc18 Proteins - genetics
Munc18 Proteins - metabolism
Physiological aspects
Plant genetics
Plant Roots - genetics
Plant Roots - metabolism
Protein Transport - genetics
Proteins
Transport Vesicles - genetics
Transport Vesicles - metabolism
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Summary:PIN-FORMED (PIN) proteins localize asymmetrically at the plasma membrane and mediate intercellular polar transport of the plant hormone auxin that is crucial for a multitude of developmental processes in plants. PIN localization is under extensive control by environmental or developmental cues, but mechanisms regulating PIN localization are not fully understood. Here we show that early endosomal components ARF GEF BEN1 and newly identified Sec1/Munc18 family protein BEN2 are involved in distinct steps of early endosomal trafficking. BEN1 and BEN2 are collectively required for polar PIN localization, for their dynamic repolarization, and consequently for auxin activity gradient formation and auxin-related developmental processes including embryonic patterning, organogenesis, and vasculature venation patterning. These results show that early endosomal trafficking is crucial for cell polarity and auxin-dependent regulation of plant architecture.
ISSN:1553-7404
1553-7390
1553-7404
DOI:10.1371/journal.pgen.1003540