Peptidomic Identification of Serum Peptides Diagnosing Preeclampsia

We sought to identify serological markers capable of diagnosing preeclampsia (PE). We performed serum peptide analysis (liquid chromatography mass spectrometry) of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a...

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Published in:PloS one 2013-06, Vol.8 (6), p.e65571-e65571
Main Authors: Wen, Qiaojun, Liu, Linda Y, Yang, Ting, Alev, Cantas, Wu, Shuaibin, Stevenson, David K, Sheng, Guojun, Butte, Atul J, Ling, Xuefeng B
Format: Article
Language:English
Subjects:
Acids
Adult
alpha 1-Antitrypsin - chemistry
Analysis
Apolipoprotein L1
Apolipoproteins
Apolipoproteins - chemistry
Biology
Biomarkers
Biomarkers - blood
Blood Proteins - chemistry
Chromatography
Chromatography, Liquid - methods
Developmental biology
Differential diagnosis
Ethnicity
Female
Fibrin
Fibrinogen
Gene expression
Glycoproteins - chemistry
Growth factors
Humans
Hypertension
Kininogens
Kininogens - chemistry
Lipoproteins, HDL - chemistry
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medical diagnosis
Medicine
Pediatrics
Peptide Fragments - blood
Peptides
Placenta
Pre-eclampsia
Pre-Eclampsia - blood
Pre-Eclampsia - diagnosis
Preeclampsia
Pregnancy
Pregnant women
Proteinase Inhibitory Proteins, Secretory - chemistry
Proteins
Scientific imaging
Sensitivity
Sensitivity and Specificity
Surgery
Tandem Mass Spectrometry - methods
Thymosin - chemistry
Training
Trypsin
Trypsin inhibitors
Urine
Womens health
Young Adult
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cited_by cdi_FETCH-LOGICAL-c758t-9841280cdc77048bee7d3308d38f436a60106d17ce6dce97244e353b3549b7593
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container_end_page e65571
container_issue 6
container_start_page e65571
container_title PloS one
container_volume 8
creator Wen, Qiaojun
Liu, Linda Y
Yang, Ting
Alev, Cantas
Wu, Shuaibin
Stevenson, David K
Sheng, Guojun
Butte, Atul J
Ling, Xuefeng B
description We sought to identify serological markers capable of diagnosing preeclampsia (PE). We performed serum peptide analysis (liquid chromatography mass spectrometry) of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a testing set. Differential serum peptide profiling identified 52 significant serum peptides, and a 19-peptide panel collectively discriminating PE in training sets (n = 21 PE, n = 21 control; specificity = 85.7% and sensitivity = 100%) and testing sets (n = 10 PE, n = 10 control; specificity = 80% and sensitivity = 100%). The panel peptides were derived from 6 different protein precursors: 13 from fibrinogen alpha (FGA), 1 from alpha-1-antitrypsin (A1AT), 1 from apolipoprotein L1 (APO-L1), 1 from inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), 2 from kininogen-1 (KNG1), and 1 from thymosin beta-4 (TMSB4). We concluded that serum peptides can accurately discriminate active PE. Measurement of a 19-peptide panel could be performed quickly and in a quantitative mass spectrometric platform available in clinical laboratories. This serum peptide panel quantification could provide clinical utility in predicting PE or differential diagnosis of PE from confounding chronic hypertension.
doi_str_mv 10.1371/journal.pone.0065571
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We performed serum peptide analysis (liquid chromatography mass spectrometry) of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a testing set. Differential serum peptide profiling identified 52 significant serum peptides, and a 19-peptide panel collectively discriminating PE in training sets (n = 21 PE, n = 21 control; specificity = 85.7% and sensitivity = 100%) and testing sets (n = 10 PE, n = 10 control; specificity = 80% and sensitivity = 100%). The panel peptides were derived from 6 different protein precursors: 13 from fibrinogen alpha (FGA), 1 from alpha-1-antitrypsin (A1AT), 1 from apolipoprotein L1 (APO-L1), 1 from inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), 2 from kininogen-1 (KNG1), and 1 from thymosin beta-4 (TMSB4). We concluded that serum peptides can accurately discriminate active PE. 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We performed serum peptide analysis (liquid chromatography mass spectrometry) of 62 unique samples from 31 PE patients and 31 healthy pregnant controls, with two-thirds used as a training set and the other third as a testing set. Differential serum peptide profiling identified 52 significant serum peptides, and a 19-peptide panel collectively discriminating PE in training sets (n = 21 PE, n = 21 control; specificity = 85.7% and sensitivity = 100%) and testing sets (n = 10 PE, n = 10 control; specificity = 80% and sensitivity = 100%). The panel peptides were derived from 6 different protein precursors: 13 from fibrinogen alpha (FGA), 1 from alpha-1-antitrypsin (A1AT), 1 from apolipoprotein L1 (APO-L1), 1 from inter-alpha-trypsin inhibitor heavy chain H4 (ITIH4), 2 from kininogen-1 (KNG1), and 1 from thymosin beta-4 (TMSB4). We concluded that serum peptides can accurately discriminate active PE. Measurement of a 19-peptide panel could be performed quickly and in a quantitative mass spectrometric platform available in clinical laboratories. This serum peptide panel quantification could provide clinical utility in predicting PE or differential diagnosis of PE from confounding chronic hypertension.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23840341</pmid><doi>10.1371/journal.pone.0065571</doi><tpages>e65571</tpages><oa>free_for_read</oa></addata></record>
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subjects Acids
Adult
alpha 1-Antitrypsin - chemistry
Analysis
Apolipoprotein L1
Apolipoproteins
Apolipoproteins - chemistry
Biology
Biomarkers
Biomarkers - blood
Blood Proteins - chemistry
Chromatography
Chromatography, Liquid - methods
Developmental biology
Differential diagnosis
Ethnicity
Female
Fibrin
Fibrinogen
Gene expression
Glycoproteins - chemistry
Growth factors
Humans
Hypertension
Kininogens
Kininogens - chemistry
Lipoproteins, HDL - chemistry
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Medical diagnosis
Medicine
Pediatrics
Peptide Fragments - blood
Peptides
Placenta
Pre-eclampsia
Pre-Eclampsia - blood
Pre-Eclampsia - diagnosis
Preeclampsia
Pregnancy
Pregnant women
Proteinase Inhibitory Proteins, Secretory - chemistry
Proteins
Scientific imaging
Sensitivity
Sensitivity and Specificity
Surgery
Tandem Mass Spectrometry - methods
Thymosin - chemistry
Training
Trypsin
Trypsin inhibitors
Urine
Womens health
Young Adult
title Peptidomic Identification of Serum Peptides Diagnosing Preeclampsia
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