Triple negative breast cancers have a reduced expression of DNA repair genes

DNA repair is a key determinant in the cellular response to therapy and tumor repair status could play an important role in tailoring patient therapy. Our goal was to evaluate the mRNA of 13 genes involved in different DNA repair pathways (base excision, nucleotide excision, homologous recombination...

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Bibliographic Details
Published in:PloS one 2013-06, Vol.8 (6), p.e66243-e66243
Main Authors: Ribeiro, Enilze, Ganzinelli, Monica, Andreis, Daniele, Bertoni, Ramona, Giardini, Roberto, Fox, Stephen B, Broggini, Massimo, Bottini, Alberto, Zanoni, Vanessa, Bazzola, Letizia, Foroni, Chiara, Generali, Daniele, Damia, Giovanna
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Anemia
Biology
Biomarkers
Breast cancer
Cancer
Cancer genetics
Cancer therapies
Care and treatment
Cell cycle
CHK1 gene
CHK1 protein
Comparative analysis
Correlation analysis
Defects
Deoxyribonucleic acid
DNA
DNA methylation
DNA repair
DNA Repair - genetics
Down-Regulation
Estrogens
Fanconi Anemia - genetics
Fanconi syndrome
Fanconi's anemia
Female
Gene expression
Gene Expression Regulation, Neoplastic
Genes
Homologous recombination
Homologous Recombination - genetics
Homology
Humans
Laboratories
Lung cancer
Medical prognosis
Medicine
Middle Aged
mRNA
Mutation
Nucleotide excision repair
Paraffin
Pathways
Pharmacology
Poly(ADP-ribose) polymerase
Receptors, Estrogen - metabolism
Regression analysis
Repair
RNA
RNA, Messenger - genetics
RNA, Messenger - metabolism
Survival
Therapy
Triple Negative Breast Neoplasms - genetics
Triple Negative Breast Neoplasms - metabolism
Triple Negative Breast Neoplasms - pathology
Tumors
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Summary:DNA repair is a key determinant in the cellular response to therapy and tumor repair status could play an important role in tailoring patient therapy. Our goal was to evaluate the mRNA of 13 genes involved in different DNA repair pathways (base excision, nucleotide excision, homologous recombination, and Fanconi anemia) in paraffin embedded samples of triple negative breast cancer (TNBC) compared to luminal A breast cancer (LABC). Most of the genes involved in nucleotide excision repair and Fanconi Anemia pathways, and CHK1 gene were significantly less expressed in TNBC than in LABC. PARP1 levels were higher in TNBC than in LABC. In univariate analysis high level of FANCA correlated with an increased overall survival and event free survival in TNBC; however multivariate analyses using Cox regression did not confirm FANCA as independent prognostic factor. These data support the evidence that TNBCs compared to LABCs harbour DNA repair defects.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0066243