Chromosomal Mapping of Repetitive DNAs in the Grasshopper Abracris flavolineata Reveal Possible Ancestry of the B Chromosome and H3 Histone Spreading

Supernumerary chromosomes (B chromosomes) occur in approximately 15% of eukaryote species. Although these chromosomes have been extensively studied, knowledge concerning their specific molecular composition is lacking in most cases. The accumulation of repetitive DNAs is one remarkable characteristi...

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Bibliographic Details
Published in:PloS one 2013-06, Vol.8 (6), p.e66532-e66532
Main Authors: Bueno, Danilo, Palacios-Gimenez, Octavio Manuel, Cabral-de-Mello, Diogo Cavalcanti
Format: Article
Language:English
Subjects:
Abracris flavolineata
Animals
B chromosome
Biology
Chromosome Mapping
Chromosomes
Chromosomes, Insect
Conservation
Cytogenetic Analysis
Deoxyribonucleic acid
DNA
DNA probes
Female
Gene mapping
Gene sequencing
Genomes
Genomics
Grasshoppers
Grasshoppers - cytology
Grasshoppers - genetics
Histones - genetics
In Situ Hybridization, Fluorescence
Male
Males
Meiosis
Multigene Family
Repetitive Sequences, Nucleic Acid
RNA, Ribosomal, 18S - genetics
RNA, Small Nuclear - genetics
rRNA 18S
snRNA
Supernumerary chromosomes
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Summary:Supernumerary chromosomes (B chromosomes) occur in approximately 15% of eukaryote species. Although these chromosomes have been extensively studied, knowledge concerning their specific molecular composition is lacking in most cases. The accumulation of repetitive DNAs is one remarkable characteristic of B chromosomes, and the occurrence of distinct types of multigene families, satellite DNAs and some transposable elements have been reported. Here, we describe the organization of repetitive DNAs in the A complement and B chromosome system in the grasshopper species Abracris flavolineata using classical cytogenetic techniques and FISH analysis using probes for five multigene families, telomeric repeats and repetitive C0t-1 DNA fractions. The 18S rRNA and H3 histone multigene families are highly variable and well distributed in A. flavolineata chromosomes, which contrasts with the conservation of U snRNA genes and less variable distribution of 5S rDNA sequences. The H3 histone gene was an extensively distributed with clusters occurring in all chromosomes. Repetitive DNAs were concentrated in C-positive regions, including the pericentromeric region and small chromosomal arms, with some occurrence in C-negative regions, but abundance was low in the B chromosome. Finally, the first demonstration of the U2 snRNA gene in B chromosomes in A. flavolineata may shed light on its possible origin. These results provide new information regarding chromosomal variability for repetitive DNAs in grasshoppers and the specific molecular composition of B chromosomes.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0066532